| Project/Area Number |
03554017
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
構造化学
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TANAKA Nobuo Tokyo Institute of Technology, Faculty of Bioscience and Biotechnology, Professor, 生命理工学部, 教授 (50032024)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIO Hisakazu Rigaku Denki Co. Ltd., Research Fellow, 製品開発部, 主任研究員
SATO Mamoru Osaka University, Institute for Protein Research, Assistant Professor, 蛋白質研究所, 助手 (60170784)
UEKI Tatsuo The Institute of Physical and Chemical Research, Chief scientist, 主任研究員 (30029954)
KATSUBE Yukiteru Osaka University, Institute for Protein Research, Professor, 蛋白質研究所, 教授 (20032013)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 1992: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥12,000,000 (Direct Cost: ¥12,000,000)
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| Keywords | Small angle scattering / Time resolved measurement / Structure in solution / Imaging plate / 蛋白質の動的構造 / イメ-ジングプレ-ト |
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| Research Abstract |
The small-angle analysis is a powerful method to understand the structure of the molecule, in the sense of the shape, size and so on. Generally, the intensity of the scattering is very weak, and the high sensitive detector is necessary to observe the intensity accurately. In the present project, we have developed the new X-ray detector suitable for the small-angle scattering experiment. The imaging plate, which was developed by Fuji photo Co. Ltd. and is utilized for the single crystal diffractometer, was installed in the new detector. The plate was 115x190mm in size and was rolled cylindrically on the drum. One direction of the plate is for the scattering angle of the data, and the other is along the elapsed time for the time resolved measurement. The interval of the data along the drum was varied by changing the rotating speed of the drum from 440 to 0 rpm.. The pixel size for reading the plate was 0.1mmx0.1mm, and 4.5MB data were observed per plate were transfered to the computer th
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rough the high speed ADC. The data in the whole plane was measured to estimate the variation dependent on the place of the plane. The accuracy was estimated to be below 3%. The feeding phenomenon was found to take the serious problem for the time resolved measurement, because the relative intensity was not estimated correctly inspire of the splendid data for each measurement. The analytical interpretation of the observed data showed that the fading was approximated by exp(-bt^<1/2>).
The computer controls the detector through the RS-232C, and saves the data to the disc through the SCSI interface. The observed data can be displayed on the monitor display. The X-ray was focused with a nickel-coated mirror and the stopped flow cell was applied for the time resolved measurement as a sample holder of the protein solution. The program for the analysis of the small angle scattering were also installed on the computer. Although the basic data for the time-resolved structural studies were obtained, the accuracy of the analysis is not known exactly. The further studies will prove the ability of the new detector. Less
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