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Development of Local Anesthetic Sensor

Research Project

Project/Area Number 03557066
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionKAGAWA MEDICAL SCHOOL

Principal Investigator

OGLI Kenji  Kagawa Medical School, Professor, 医学部, 教授 (40079934)

Co-Investigator(Kenkyū-buntansha) MIYATAKE Akira  Kagawa Medical School Assistant Professor, 医学部, 講師 (80211598)
YOKONO Satoshi  Kagawa Medical School Hospital Asosiat Professor, 医学部・附属病院, 助教授 (70106425)
SATAKE Hiromu  University of Tokushima, Faculty of Engineering, Professor, 工学部, 講師 (10124801)
KANESHINA Shouji  University of Tokushima, Faculty of Engineering, Assistant Professor, 工学部, 教授 (80035617)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥16,900,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1991: ¥12,000,000 (Direct Cost: ¥12,000,000)
KeywordsLocal anesthetics / Ion selective membrane / Coated wire electrode / リドカイン / in vivo / 血中濃度 / 被覆線電極
Research Abstract

1.Local anesthetic-sensitive electrodes : The coated-wire electrodes wereprepared by coating copper wires (0.8mm in diameter) with membranes of PVC matrix included the ion pairs, dioctylphtalate, and tetrahydrofuran. A microelectrodes (0.05mm in diameter) were also developed.The reference electrode was made of Ag/AgCl connecting a normal saline agar bridge.
2.Sensitivity : These sensors were sonsitive to local anesthetics of concentration range 10^<-6> to 10^<-2> mol/L.The detecting limitation was 10^<-8> mol/L.The electrodes rsponded to the concentrationchanges of protonated form of local anesthetics according to the Henderson-Hasselbalch equation. Temperature influenced the Nernstian-slopes.
3.Selectivity : No ions in the body affected the electrode potentials. The more hydrophobic the electrode's localanesthetic, the less it is interfered with by other local anesthetic molecules.
4.Interaction with albumin : Albumin itself did not affect the baseline potential of the electrode. As the electrode potential changed with the decreasein free anesthetic molecules after binding with albumin, it's easy to get the number of binding sites for local anesthetics on one albumin molecule. Five dibucaine molecules binds on one albumin molecule.
5.In vivo study : According to the two compartment model analysis, V_1=0.111/kg, V_2=0.121/kg, k_<12>=1.88/min, k_<21>=0.88/min, k_<10>=1.88/min in rabbit study. The concentrations from our sensor agreed with those from HPLC analysis.
6.Effects of metabolites of local anesthetics : The metabolites of lidocaine influenced the electrode potentials. The selectivities of MEGX and hydroxylidocaine were 1*10^<0.6-0.8>.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Hiromu SATAKE: "Coated wire electrodes sensitive to local anesthetic catious and their application to potentiometric determination." Bulletin of the Chemical Society of Japan. 64. 3029-3034 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 佐竹弘: "局所麻酔薬ブピバカインに応答する被覆線型電極の試作とその生体での応用" 分析科学. 41. 573-580 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Atsuko Yokono: "Local anesthetic-sensitive electrodes:preparation of coated-wire electrodes and their basic properties in vitro." Anesthesia and Analgesia. 75. 1063-1069 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 金品昌志: "高圧下における生体モデル膜の機能" J.Soc.Materials Sci.Japan. 41. 336-341 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 金品昌志: "Effects of pressure and ethanol on the phase behavior of dipalmitoyl phospahtidylcholine multilamellar vesicles." Chem.Letters. 1963-1966 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 金品昌志: "Colloidal properties of aqueous local anesthetic tetracaine solutions." Colloid Surfaces A. 71. 135-140 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiromu SATAKE,et al.: "Coated wire electrodes sensitive to local anesthetic cations and their application to potehtiometric determination." Bull Chem Soc Japan. 64. 3029-3034 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Atsuko Yokono, et al: "Local anesthetic-sensitive electrodes : preparation of coated-wire electrodes and their basic properties in vitro." Anesth & Analg. 75. 1063-1069 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Atsuko Yokono et al.: "Continuous measurement of lidocaine in vivo" Anesthesiology. 77. A484 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shouji KANESHINA et al: "Effects of pressure and ethanol on the phase behavior of dipalmitoy1 phospahtidylcholine multilamellar vesicles." Chem.Letters. 1963-1966 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shouji KANESHINA et al: "Colloidal properties of aqueous local anesthetic tetracaine solutions." Colloid Surfaces A. 71 :. 135-140 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 金品昌志: "Colloidal properties of aqueous local anesthetic tetracaine solutions." Colloid Surfaces A. 71. 135-140 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 金品昌志: "高圧下における生体モデル膜の機能" J.Soc.Materials Sci.Japan. 41. 336-341 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 金品昌志: "Effect s of pressure and ethanol on the phase behavior of dipalmitoyl phospahtidylcholine multilamellar vesicles." Chem.Letters. 1963-1966 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 佐竹弘: "局所麻酔薬ブピバカインに応答する被覆線型電極の試作とその生体への応用" 分析化学. 41. 571-578 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 横野諭: "リドカイン血中濃度測定法-電極法とHPLCの比較-" 麻酔. 42. S754 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 横野諭: "Continuous measurement of lidocaine in vivo" Anesthesiology. 77. A484 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Atsuko Yokono: "Local anesthetic-sensitive electrodes:preparation of coated-wire electrodes and their basic properties in vitro." Anesthesia and Analgesia. 75. 1063-1069 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 佐竹 弘: "局所麻酔薬ブピバカインに応答する被覆線型電極の試作とその生体での応用" 分析化学. 41. 573-580 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Atsuko Yokono: "Continuous measurement of lidocaine in vivo" Anesthesiology. 77. A484 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 横野 敦子: "局所麻酔薬電極を用いた家兎血中リドカイン濃度の体内連続測定" 麻酔. 41. S667 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiromu SATAKE;Takahiro MIYATA;Shoji KANESHINA: "Coated wire electrodes sensitive to local anesthetic cations and their application to potentiometric determination." Bulletin of the Chemical Society of Japan. 64. 3029-3034 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Atsuko YOKONO;Satoshi YOKONO;Kenji OGLI et al: "Lidocaine sensitive electrode" Anesthesia and Analgesia.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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