|Budget Amount *help
¥2,000,000 (Direct Cost : ¥2,000,000)
Fiscal Year 1992 : ¥1,000,000 (Direct Cost : ¥1,000,000)
Fiscal Year 1991 : ¥1,000,000 (Direct Cost : ¥1,000,000)
1. Chondroitin sulfate proteoglycans in the central and peripheral nervous system of postnatal and fetal rats were studied. Six core proteins of the proteoglycans of which composition changed during the postnatal development were taken, suggesting that appearance of proteoglycans takes a part in various aspect of development in the nervous system
2. One of them that was the major core in adults, but was contained at very low level in neonates, seemed to be produced by glial cells. By contrast, the proteoglycan with another core that was predominant in neonates was produced by neuronal cells. In the cerebellum, some of neurons such as Purkinje cells, Golgi cells, and neurons in the deep nuclei contained the neuronal proteoglycan, where as granule cells in the external and internal granular layers, basket cells and other small cells in the molecular layer did not contain it, indicating that different kinds of cells had different types of proteoglycans.
3. In fetuses, the neuronal proteoglycan was seen in fibers of the central and peripheral nerves. By contrast, the distribution of the proteoglycan in fibers was not seen in adult nervous system. Thus, one proteoglycan likely changed its intracellular distribution during the development in the nervous system.
Theses results suggest that individual proteoglycans are involved in various aspect of the development in the nervous system.