• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of T cell growth-signal transduction and its abnormality

Research Project

Project/Area Number 03670172
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAMBA Yuziro  Inst. for Virus Res., Kyoto Univ. Associate Prof., ウイルス研究所, 助教授 (50027322)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsprotein phosphorylation / T lymphocyte / 1-plastin / IL 2 / actin-binding protein / calcium-binding protein / monoclonal antibody / microfilament / シグナル伝達 / リンパ球の増殖 / カルシウム
Research Abstract

65-kilodalton protein has been characterized as an interleukin 2-stimulated phosphoprotein in human T cells. We determined the complete primary structure of p65 based on the cDNA isolated from a human T lymphocyte (KUT-2) cDNA library. Analysis of p65 sequence indicated that p65 is multidomain molecule involving at least three intriguing domains; two putative calcium-binding sited along the N- terminal 80 amino acid residues; a putative calmodulin-binding site following the calcium binding region; and two tandem repeats of putative actin-binding domains in its middle and C-terminal parts, each containing approximately 240 residues. This domain structure closely resembles a chicken intestinal microvilli protein, fimbrin, that bundles actin filaments. It was observed that unphosphorylated L-plastin isolated from human T cells bundles F-actin just as fimbrin does. L-plastin acted on T cell beta-actin, but hardly acted on muscle alpha-actin or chicken gizzard gamma-actin, whereas fimbrin bundles alpha-actin. Unlike fimbrin, L-plastin's actin-bundliung action was strictly calcium-dependent: the bundles were formed at pCa 7, but not at pCa 6. Under suitable conditions, approximately one molecule of L-plastin bound to 8 molecules of actin monomer in the actin filament.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Namba,Y.,Ito,M.,Zu,Y.,Shigesada,K.〓 Maruyama,K.: "Human T cell l-plastin bundles actin filaments in a calcium dependent manner." J.Biochemistry. 112. 503-507 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Haga,S.,Shigesada,K.,Namba,Y.,Tanaka,H.,〓 Hilgers,J.: "Polyclonal and monoclonal antibodies monospecific to MMTV LTR orf protein produced in E.coli." Cancer Letter. 65. 201-207 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kondo,S.,Miyatake,S.,Oda,Y.,Kikuchi,H.,〓 Namba,Y.,: "Analysis of the close relationship between human astrocytoma-specific antigens detected by murine monoclonal antibodies and c-kit proto-oncogene products." Biochem.Biophys.Res.Comm.,. 182. 474-480 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kondo,S.,Miyatake,S.,Kikuchi,H.,Shamoto,M.〓 Namba,Y.: "Human glioma-specific antigens detected by monoclonal antibodies." Neurosurgery. 30. 506-511 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kondo,S.,Miyatake,S.,Kikuchi,H.,Oda,Y.〓 Namba,Y.,: "Mechanism of interferon gamma-induced protection of human gliosarcoma cells from lymphokine-activated killer lysis." Neurosurgery. 31. 534-540 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 難波 雄二郎: "Tリンパ球の増殖シグナルの伝達とその異常" 病態生理. 11. 882-884 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 難波 雄二郎(分担報筆)野々村 禎昭・小幡 邦彦 編集: "新生理学大系・(7)発生と分化の生理学「抗体産生細胞の分化」" 医学書院, 313(90-97) (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Namba, Y., Ito, M., Zu, Y., Shigesada, K. and Maruyama K.: "Human T cell 1-plastin bundles actin filaments in a calcium dependent manner." J. Biochemistry. 112. 503-507 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Haga, S., Shigesada, K., Namba, Y., Tanaka, H. and Hilgers, J.: "Polyclonal and monoclonal antibodies monospecific to MMTV LTR orf protein produced in E. coli." Cancer Letter. 65. 201-207 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kondo, S., Miyatake, S., Oda, Y., Kikuchi, H. and Namba, Y.: "Analysis of the close relationship between human astrocytoma-specific antigens detected by murine monoclonal antibodies and c-kit proto-oncogene products." Biochem. & Biophys. Res. Comm.182. 474-480 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kondo, S., Miyatake, S., Kikuchi, H., Shamoto, M. and Namba, Y.: "Human glioma-specific antigens detected by monoclonal antibodies." Neurosurgery. 30. 506-511 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kondo, S., Miyatake, S., Kikuchi, H., Oda, Y. and Namba, Y.: "Mechanism of interferon gamma-induced protection of human gliosarcoma cells from lympokine-activated killer lysis." Neurosurgery. 31. 534-540 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Namba,Y.,Ito,M.,ZU,Y.,Shigesada,K.& Maruyama,K.: "Human T cell 1-Plastin bundles actin filaments in a calcium dependent manner." J.Biochemistry. 112. 503-507 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Haga,S.,Shigesada,K.,Nambo,Y.,Tanaka,H. & Hilgers,J.: "Polyclonal and monoclonal antibodies monospecific to MMTV LTR orf protein produced in E. coli." Cancer Letter. 65. 201-207 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kondo,S.,Miyatake,S.,Oda,Y.,Kikuchi H. & Namba,Y.: "Analysis of the close relationship between human astrocytoma-specific antigens detected by murine mono-clonal antibodies and c-kit proto-oncogene products." Biochem.Biophys.Res.Comm.182. 474-480 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kondo,S.,Miyatake,S.,Kikuchi,H.,Shamoto,M. & Namba,Y.: "Human glioma-specific antigens detected by monoclonal antibodies." Neurosurgery. 30. 506-511 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kondo,S.,Miyatake,S.,Kikuchi,H.,Oda,Y. & Namba,Y.: "Mechanism of interferon gamma-induced protection of human gliosarcoma cells from lymphokine-activated killer lysis." Neurosurgery. 31. 534-540 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 難波 雄二郎: "Tリンパ球の増殖シグナルの伝達とその異常" 病態生理. 11. 882-884 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Namba,Y.;Ito,M.;Zu,Y.;Shigesada,K;& Maruyama,K.: "Human T cell 1ーplastin bundles actin filaments in a calcium dependent manner" Biochemistry. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kondo,S.,Miyatake,S.,Kikuchi,H.& Namba,Y.: "Analysis of the close relationship between human astrocytomaspecific antigens detected by murine monoclonal antibodies and cーkit protoーoncogene product" Biochemical and Biopysical Research Communications. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kondo,S.,Miyatake,S.,Kikuchi,H.& Namba,Y.: "Human gliomaーspecific antigens detected by monoclonal antibodies" Neurosurgery. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 難波 雄二郎: "新生理科学大系(7).抗体産生細胞の分化(分担執筆)" 医学書院 編集:野々村禎昭・小幡邦彦, 90-97 (1991)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi