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Dynamic changes of RNA and protein synthesis by neutrophils during the course of acute inflammation in rabbits.

Research Project

Project/Area Number 03670177
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKUMAMOTO UNIVERSITY

Principal Investigator

OHKAWARA Susumu  Kumamoto University School of Medicine, Department of Pathology Research associate, 医学部, 助手 (10094088)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsInflammation / Polymorphonuclear leukocyte / Protein synthesis / Subtraction library / Inflammatory mediators / 差引きライブラリ-
Research Abstract

Neutrophils have long been considered to be terminally differentiated end cells and to possess little RNA and protein synthesis capability. Using a casein-induced rabbit peritonitis, we found that neutrophils synthesize interleukin 1beta(IL-1beta)de novo only at early stages of inflammation. This observation suggested that protein synthesis of neutrophils is involved with inflammatory reaction. With the focus on RNA/protein synthesis capability by neutrophils and its dynamic change during the inflammatory process, we carried out gene library subtraction studies. We performed gene subtraction with the ss cDNA library hybridization method and the resulted clones of 5 hr-minus 24 hr-neutrophils were further confirmed by differential hybridization. The cDNA from candidate clones were converted to sense- and anti-sense-RNA and using these RNA probes, we performed Northern blot and cytoplasmic slot blot analyses.
Immune activation gene 2(Act-2), MIF related protein-8(MRP-8), MRP-14, fMLP rece … More ptor (fMLPR) and gamma-actin homologues were isolated as genes representing neutrophils at an early inflammatory stage. Ferritin light(L)chain cDNA was cloned as the gene representing neutrophils at a late inflammatory stage. In addition, mRNA expression of rabbit neutrophil attractant/activation protein-1/interleukin 8(NAP-1/IL-8), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor alpha(TNFalpha)and ferritin heavy(H)chain mRNASs expression during inflammation were examined by cytoplasmic dot blot analysis. The patterns of mRNAs of Act-2, NAP-1/IL-8, MRP-14 and gamma-actin homologues were induced immediately after the onset of inflammation. This pattern is similar to that of IL-1beta mRNA expression. Pattern2 : mRNA expression of the ferritin H chain in neutrophils reached a peak at intermediate periods (12hr)of inflammation, with a monophagic pattern. Pattern3 : the level of ferritin L chain mRNA increased with progression of the inflammatory process. The mRNA expressions of TNFalpha and MCP-1 was not significant. These data suggest that neutrophils contribute to the defense reactions by synthesizing various proteins at the site of inflammation. Even in a later stage of inflammation, neutrophils were viable and retain protein synthetic capability, although they are generally believed to have a very short life-span and may die at a different site of inflammation. Less

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Ohkawara S,Matsukawa and Yoshinaga M: "Neutrophils as the major producer of interleukin l and its inhibitor at the inflammatory site in rabbits." Archiv.Immunol.Therap.Exp.40. 11-16 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Goto F,Goto K,Miyata T,Ohkawara S.,Takao T,Mori S,Furukawa S,Maeda T,Iwanaga S,Shimonishi Y and Yoshinaga M: "Interleukin-l receptor antagonist in inflammatory exudate cells of rabbits.Production,purification and determination of primary structure." Immunology. 77. 235-244 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsukawa A,Mori S,Ohkawara S,Maeda T,Tanase S,Edamitsu S,Yanagi F and Yoshinaga M: "Production,Purification and characterization of a rabbit recombinant IL-l receptor antagonist." Biomed.Res.13. 269-277 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsukawa A,Ohkawara S,Maeda T,Takagi K and Yoshinaga M: "Production of interleukin-l and interleukin-l receptor antagonist and the pathological significance in lipopolysaccharide-induced arthritis in rabbits." J.Clin.Exp.Immunol.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 大河原 進、松川 昭博: "炎症の場における好中球の蛋白合成能。" 細胞. 24. 220-224 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 大河原 進、松川 昭博、枝光 淳、柳 文治: "炎症局所で見出したIL-l抑制因子の構造と機能" 病態生理. 11. 962-966 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsukawa A,Ohkawara S and Yoshinaga M: "Intractable vasculitis syndromes" Hokkaido University Press, 131-138 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ohkawara S, Matsukawa A and Yoshinaga M: "Neutrophils as the major producer of interleukin 1 and its inhibitor at the inflammatory site in rabbits." Archiv. Immunol. Therap. Exp. 40. 11-16 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Goto F, Goto K, Miyata T, Ohkawara S., Takao T, Mori S, Furukawa S, Maeda T, Iwanaga S, Shimonishi Y and Yoshinaga M: "Interleukin-1 receptor antagonist in inflammatory exudate cells of rabbits. Production, purification and determination of primary structure." Immunology. 77. 235-244 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsukawa A, Mori S, Ohkawara S, Maeda T, Tanase S, Edamitsu S, Yanagi F and Yoshinaga M: "Production, purification and characterization of a rabbit recombinant IL-1 receptor antagonist." Biomed. Res.13. 269-277 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsukawa A, Ohkawara S, Maeda T, Takagi K and Yoshinaga M: "Production of interleukin-1 and interleukin-1 receptor antagonist and the pathological significance in lipopolysaccharide-induced arthritis in rabbits." J. Clin. Exp. Immunol.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsukawa A, Ohkawara S and Yoshinaga M: Hokkaido University Press. Intractable vasculitis syndromes, 131-138 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ohkawara S,Matsukawa A and Yoshinaga M: "Neutrophils as the major producer of interleukin 1 and its inhibitor at the inflammatory site in rabbits." Archiv.Immunol.Therap.Exp.40. 11-16 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Goto F,Goto K,Miyata T,Ohkawara S.,Takao T,Mori S,Furukawa S,Maeda T,Iwanaga S,Shimonishi Y and Yoshinaga M: "Interleukin-1 receptor antagonist in inflammatory exudate cells of rabbits.Production,purification and determination of primary structure." Immunology. 77. 235-244 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsukawa A,Mori S,Ohkawara S,Maeda T,Tanase S,Edamitsu S,Yanagi F and Yoshinaga M: "Production,purification and characterization of a rabbit recombinant IL-1 receptor antagonist." Biomed.Res.13. 269-277 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsukawa A,Ohkawara S,Maeda T,Takagi K and Yoshinaga M: "Production of interleukin-1 and interleukin-1 receptor antagonist and the pathological significance in lipopolysaccharide-induced arthritis in rabbits." J.Clin.Exp.Immunol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 大河原 進、松川 昭博: "炎症の場における好中球の蛋白合成能。" 細胞. 24. 220-224 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 大河原 進、松川 昭博、枝光 淳、柳 文治: "炎症局所で見出したIL-1抑制因子の構造と機能" 病態生理. 11. 962-966 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsukawa A,Ohkawara S and Yoshinaga M: "Intractable vasculitis syndromes" Hokkaido University Press, 131-138 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] F.Goto,K.Goto.T.Miyata,S.Ohkawara,T.Takeo,S.Mori,S.Iwanaga,M.Yoshinaga: "Interleukin 1 receptor antagonist in inflammatory exudate cells of rabbits.Produection purification and determimation of primary" Immunology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Matsukawa,S.Mori,S.Ohkawara,T.Maeda,M.Yoshinaga: "Proceuction,parification and characterization of a rabbit recommnant ILー4 receptor antagonist." Biochem.Biopbys.Res.Cammu.

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Sagara,S.Mori S.Ohkawara,K.Takagi,M.Yoshinaga: "A limitea role of ILー1 in immume enhancement by adjuvcants." Immunology. 71. 251-257 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] O.Ranuilo X.SaezーLloreus,J.Mertsola,M.Yoshinaga,S.Ohhawara,G.H.McChacken Jr.: "Tumor necrosis factora/cachectin and interleukin 1p initiate meningeal inflammation." J.Exp.Med.172. 497-507 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大河原 進,後藤 文正,吉永 秀: "炎症性サイトカイン" 蛋白質・核酸・酵素. 36. 935-943 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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