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Studies on human complement 4 binding protein

Research Project

Project/Area Number 03670325
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

OKAMURA Seiichi  Kyushu University, Medicine, Assistant Professor, 医学部, 講師 (20136435)

Co-Investigator(Kenkyū-buntansha) KUBOTA Akira  Kyushu University, Medicine, Physician, 医学部, 医員
HARADA Naoki  Kyushu University, Medicine, Physician, 医学部, 医員
IKEMATSU Wataru  Kyushu University, Medicine, Physician, 医学部, 医員
SHIMODA Kazuya  Kyushu University, Medicine, Physician, 医学部, 医員
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsComplement regulatory protein / Genomic organization / Complement 4 binding-protein / Acute phase reactive protein / Monoclonal antibody / Rheumatoid arthritis / Cytokine / モノクロ-ナル抗体
Research Abstract

1. Purification of human C4-binding protein (C4BP) and development of antibody against the purified C4BP Human C4BPalpha was purified from 1.5L of plasma by multiple steps including BaC12 precipitation, Intralipid absorption, ultracentrifugation, ethanol/ethel extraction, column chromatographies (DE52,S400 and S300). Antibodies against this protein were raised in rabbits and mice. Monoclonal antibody (IgG) was also developed. 2. Human genomic C4BP gene Human genomic C4BPalpha gene was analyzed. This gene consists of 12 exons and spans about 40 kb. Each of the SCRs is encoded by a single exon, except for the second SCR (SCR II), which is encoded by two separate exons. The 5' flanking region was sequenced up to 380 bases upstream from the putative transcription initiation site. Several possible binding sites for transcription factors were identified. 3. Gene expression of the C4BPalpha The gene expression of the C4BP alpha in normal liver cell and hepatoma lines was demonstrated. The constitutive gene expression of C4BPalpha by a hepatoma line, HepG2, was significantly augmented by treatment with monocyte conditioned medium, 12-O-tetradecanoylphorbol-13-acetate,interleukin-6 and tumor necrosis factor but not by a calcium ionophore or interleukin-1beta. 4. Serum levels of C4BP Serum levels of C4BP were measured in patients with various autoimmune disease. Significantly high level of C4BP was observed in patients with active or LAC-positive SLE,Behcet disease, aortitis, PSS,RA and MCTD. The level of C4BP was correlated with CRP, C3 and CH50.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Matsuguchi Tetsuya: "Interleukin-6 and tumor necrosis factor-alpha fully activate liver-specific gene expression of C4b-binding protein." Biochemistry International. 23. 979-985 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aso Teijiro: "Genomic organization of the α chain of the human C4b-binding protein." Biochemical Biophysical Research Communication. 174. 222-227 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Okamura Seiichi: "The effects of recombinant human granulocyte-macrophage colony-stimulating factor on the induction of lymphokine-activated killer cells in vitro." International Journal of Immunopharmacology. 13. 587-593 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "Granulocyte colony-stimulationg factor in cerebrospinal fluid from patients with meningitis." Blood. 77. 2214-2217 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "Identification of a functional receptor for granulocyte colony-stimulating factor on platelets." Journal of Clinical Investigatiom,in press.1993.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "Granulocyte colony-stimulating factor and platelet agregation." Lancet,in press.1993.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuguchi Tetsuya: "Interleukin-6 and tumor necrosis factor-alpha fully activate liver-specific gene expression of C4b-binding protein." Biochemistry International. 23. 979-985 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aso Teijiro: "Genomic organization of the alpha chain of the human C4b-binding protein." Biochemical Biophysical Research Communication. 174. 222-227 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Okamura Seiichi: "The effects of recombinant human granulocyte-macrophage colony-stimulating factor on-the induction of lymphokineactivated killer cells in vitro." International Journal of Immunopharmacology. 13. 587-593 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "Granulocyte colony-stimulating factor in cerebrospinal fluid from patients with meningitis." Blood. 77. 2214-2217 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "Identification of a functional receptor for granulocyte colony-stimulating factor on platelets." Journal of Clinical Investigation. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "Granulocyte colony-stimulating factor and platelet agregation" Lancet. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ikematsu Wataru: "Circulating CD34_+ hematopoietic progenitors in the harvesting peripheral blood stem cells;Enhancement by recombinant human granulocyte colony-stimulating factor." Biotherapy. 5. 131-136 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimoda Kazuya: "Granulocyte colony-stimulating factor receptors on human leukemia:Biphenotypic leukemic cells posesses G-CSF receptors." Cancer Research. 52. 3252-3255 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kondo Seiji: "CD7-positive acute myeloid leukemia:Further evidence of cellular immaturity." Journal of Clinical and Experimental Oncology. 118. 386-388 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Harada Naoki: "Influence of a traditional Chinese herbal medicine,Ren-shen-yang-rong-tang (Japanese name:Ninjin-yoei-to) on production of interleukin-6 from peripheral blood mononuclear cells and bone marrow fibroblasts." International Journal of Immunotherapy. 8. 141-145 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimoda Kazuya: "Identification of a functional receptor for granulocyte colony-stimulating factor on platelets." Journal of Clinical Investigation.

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimoda Kazuya: "Granulocyte colony-stimulating factor and platelet aggregation." LANCET.

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsuguchi Tetsuya: "Interleukinー6 and tumor necrosis factorーalpha fully activate liverーspecific gene expression of C4bーbinding protein." Biochemistry International. 23. 979-985 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Aso Tejiro: "Genomic organization of the α chain of the human C4bーbinding protein." Biochemcal Biophysical Research Communication. 174. 222-227 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okamura Seiichi: "The effects of recombinant human granulocyteーmacrophage colonyーstimulating factor on the induction of lymphokineーactivated killer cells in vitro." International Jouranl of Immunopharmacology. 13. 587-593 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okamura Seiichi: "A traditional chinese herbal medicine,renーshenーyangーtang(Japanese name:ninjinーyoeiーto)augments the production of granulocyteーmacrophage colonystimulating factor from human peripheral blood mononuclear cells in vitro." International Journal of Immunopharmacology. 13. 595-598 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shimoda Kazuya: "Granulocyte colonyーstimulating factor in cerebrospinal fluid from patients with meningitis." Blood. 77. 2214-2217 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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