|Budget Amount *help
¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 1992 : ¥400,000 (Direct Cost : ¥400,000)
Fiscal Year 1991 : ¥1,200,000 (Direct Cost : ¥1,200,000)
Studies of callus induction for some mangrove species(Rhizophoraceae) were done, that had not been reported to succeed from it. Several experiments of redifferentiation were also done. Propagules of three mangrove species(Kandelia candel, Rhizophora stylosa and Bruguiera gymnorrhiza) used for experiments were collected from lriomote and Okinawa island, Okinawa prefecture, during 1991 and 92. Terminal and root merystem were also used for callus induction. Terminal and root merystem were also used for callus induction. Plant hormone and its concentration were 2,4-D and Kinetin, and these combination of 5^*10^<-5>, ^*10^<-6> and ^*10^<-7>M respectively. Culture media used was Murasige and Skoog and 0.8% agar. Both terminal and root merystem of K.candel were not recognized to induce callus tissue. Because these tissues were so labile that affected by the sterilization and died with in two weeks after transplanting. Only explant of propagule were able to recognize callus formation, especial
ly from immature ones. Several different developing stage of propagule of R.stylosa and B.gymnorrhiza studied, were recognized to produce callus from the early or young ones. Explant from mature propagules of two species were not successful by the contamination of fungi or bacteria even under heavy sterilization. All three mangrove species were not able to form callus from terminal and root merystem, but from the propagules in early or young stage of development. These facts were confirmed by following several experiments. Concentration of hormonal treatments are also determined by the same experiments. Some experiments for redifferentiation from R.stylosa callus were done under combinations of 2.4-D and IAA, and Kinetine, and each three concentrations described above. Few adventituous bud were able to differentiate from callus tissues. This condition are now under examination.
沖縄本島億首川のK.candel(メヒルギ)自生地から,propagule(発芽種子)を採種し,また同じ生息地から持ち帰ったpropaguleを水耕で培養した幼植物から葉のapical merystem及びroot merystemをえ,細胞培養の供試材料とした。ホルモンは2,-4DとKinetinとし,それぞれ5^*0-5,5^*10-6,5^*10-7Mの濃度でMurasige Skoog寒天培地を調製した。Apical merystemは置床後わずか褐変・壊死し,root merystemは完全な殺菌ができず雑菌の汚染が認められたが,ropaguleからカルスの誘導が確認された。R.stylosa(ヤエヤマヒルギ)の場合は,幼苦なpropa-guleをもちいた場合のみ,特定のホルモン濃度の組み合せからカルスの誘導が確認された。B.GYMNORrhiza(オヒルギ)からのカルス誘導実験でも,成熟したproraguleより,発芽後数cmのもののほうが雑菌の汚染がすくなく,確実にカルス誘導ができた。以上,カルス誘導に供試したRhizophoraceaeのマングローブ3種類では,いずれもpropaguleの未熟個体の成功率がたかく,terminal merystemやrootmerystemでは,消毒の影響によって組織の褐変や壊死がおこり,成功率はきわめて低かった。propaguleからは確実にカルスの誘導が可能となった。