Grant-in-Aid for Scientific Research on Priority Areas.
|Research Institution||Osaka University|
OGAWA Hideyuki Osaka University, Faculty of Sience, Prof., 理学部, 教授 (70028207)
関口 睦夫 九州大学, 生体防御医学研究所, 教授 (00037342)
柳田 充弘 京都大学, 理学部, 教授 (80025428)
OHTSUBO Eiichi University of Tokyo, Insti. of Mol. & Cellu. Biol., Prof., 分子細胞生物学研究所, 教授 (10158800)
IKEDA Hideo University of Tokyo, Insti. of Medical Sci., Prof., 医科学研究所, 教授 (10012775)
HOTTA Yasuo Nara Insti. of Sci. & Tech., Gradu. School of Biosci., Prof., 教授 (30190218)
SHIROISHI Tosihiko Nation. Insti. of Genet., Asso. Prof., 助教授 (90171058)
SHIBATA Takehiko Insti. of Physi. Chemi. Res., Chief Inves., 研究者 (70087550)
|Project Fiscal Year
1991 – 1994
Completed(Fiscal Year 1994)
|Budget Amount *help
¥42,600,000 (Direct Cost : ¥42,600,000)
Fiscal Year 1994 : ¥3,000,000 (Direct Cost : ¥3,000,000)
Fiscal Year 1993 : ¥24,000,000 (Direct Cost : ¥24,000,000)
Fiscal Year 1992 : ¥15,600,000 (Direct Cost : ¥15,600,000)
|Keywords||Genetic recombination / RecA protein / RecA homologues / Rad51 protein / Transposon / Shufflon / Illegitimate recombination / Recombination hot spot / 減数分裂期組換え / 非相同組換え / 組換えホットスポット / トランスポゾン転移酵素 / 標的遺伝子破壊 / ホリデ-構造開裂酵素 / DNAヘリカーゼ / 抗体受容体遺伝子再構成 / プラスミドシャフロンのDNA逆位 / 組み換えホットスッポット / 抗原受容体遺伝子再構成 / 相同的組換え / RecA蛋白質 / ジャイレース依存の組換え / 遺伝子ターゲッティング / シャフロン / 部位特異的組換え / トランスポゾン|
Genetic recombination is a fundamental mechanism by which parental traits of an organism are distributed to offspring. It provides a means of spreading a favorable allele and of eliminating an unfavorable allele in aspecies. Recombination must therefore have playd a crucial role in evolution of the genome. Recombination is also involved in various biological functions in a cell. It participates in repair of DNA damage, a primer formation of DNA replication, production of gene product diversities, alternative gene expression, and chromosome disjunction in meiosis I.Thus the study of recombination is crucial for thge understanding of the mechanisms of biological functions. To further our knowledge of fundamental problems of genetic recombination, a research group was organized supported by this grant. Main distingushed results obtained by this reserach group are as follows.
1. The homologues of the recombination gene, recA, were successfully cloned from yeasts, chicken, mouse and human.
A process of resolution of recombination intermediates formed by RecA to recombinant molecules are revealed.
3. A recombinaion hot spot specific to mouse female was found and identified at the nucleotide sequence level on the chromosome 17.
4. Two novel methods useful for study of genetic recombination were established.
(1) A simple and easy method to get various chimera genes between homologues using a recE pathway of E.coli.
(2) A comprehensive method to clone a specific site that made a genetic rearrangement from higher eukaryotes was estabilshed using in-gel competitive reassociation method.
5. Various kinds of recombination proteins were identified and analyzed : for instance, Dhpl (S.pombe homologue of STPbeta in S.cerevisiae), Lim15 (lily homologues of Dmcl in S.cerevisiae), and RBP-Jkappa(mouse homologue of Su(H) in D.melanogaster).
Thus a great advancement was made in analysis of basic recombination mechanism, methodology, and analysis of recombination mechanism in higher eukaryotes by this project. Less