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細胞エネルギー共役系の構築と調節

Research Project

Project/Area Number 04266102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo

Principal Investigator

安楽 泰宏  東京大学, 大学院・理学系研究科, 教授 (20012643)

Co-Investigator(Kenkyū-buntansha) 向畑 泰男  名古屋大学, 理学部, 教授 (10028110)
香川 靖雄  自治医科大学, 医学部, 教授 (30048962)
川喜田 正夫  東京都臨床医学総合研究所, 部長 (00012740)
吉田 賢右  東京工業大学, 資源化学研究所, 教授 (90049073)
二井 将光 (二井 將光)  大阪大学, 産業科学研究所, 教授 (50012646)
松原 央  大阪大学, 理学部, 教授 (00028242)
Project Period (FY) 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥56,000,000 (Direct Cost: ¥56,000,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1994: ¥20,000,000 (Direct Cost: ¥20,000,000)
Fiscal Year 1993: ¥18,100,000 (Direct Cost: ¥18,100,000)
Fiscal Year 1992: ¥14,900,000 (Direct Cost: ¥14,900,000)
Keywords細胞エネルギー / ATP合成酵素 / 液胞型ATP分解酵素 / 呼吸酵素 / 電子伝達複合体 / 輸送タンパク / オスモエンザイム / 化学浸透学説 / 化学浸透光説 / 輸送蛋白
Research Abstract

本総括班の研究実績は以下のとおりである.
1.平成7年10月,「細胞エネルギー共役系の構築と調節」(平成4年度-6年度)に係わる研究経過等の報告書をとりまとめ,同年10月の業績・成果報告会に提出し,ヒアリングを受けた.
2.運営委員会(7年9月17日および11月14-15日)を開催し,成果とりまとめの基本方針を審議した.
3.総括班主催の公開講演会「バイオエナジェテイクスの新展開:エネルギー変換系の構造とメカニクス」(7年12月19日:名古屋大学医学部・鶴友会館)を開催し,本重点領域研究の成果を発表するとともに,今後の研究推進方針を討議した.
4.合同班会議(6年12月20-22日)を開催し,全班員の研究成果を討議し,その評価を行った.
5.総括班会議を併催して研究成果の合同評価を行った.また,研究実績報告書の取りまとめの方針を決定し,同報告書を平成8年2月上梓した.

Report

(4 results)
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (284 results)

All Other

All Publications (284 results)

  • [Publications] I.Tanida: "Cooperation of calcium and vacuolar H^+-ATPase in intracellular Ca^<2+>homeostasis of yest cells." J.Biol.Chem.270. 10113-10119 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Maeda: "Roles of gastric GATA DNA-binding proteins." J.Exp.Biol.(In press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Omote: "β Subunit Glu-185 of Escheirchia coliH^+-ATPase(ATP synthase)is an esential residue for cooperative catalysis." J.Biol.Chem.270. 25656-25660 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] C.Jeanteur: "β/α Subunit interaction is required for catalysis by H^+-ATPase(ATP synthase):β Subunit amino terminal replacements suppress a γ subunit mutation having long unrelated carboxyl terminal." J.Biol.Chem.270. 22850-22854 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] R.K.Nakamoto: "The ATP synthase γ subunit:Suppresor mutagenesis reveals three helical regions involved in energy coupling." J.Biol.Chem.270. 14042-14046 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Moriyama: "Microvesicles isolated from bovine posterior pituitary accumulate norepinephrine." J.Biol.Chem.270. 11424-11429 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Moriyama: "Quinacrine mustard and lipophilic cations inhibitory to both vacuolar H^+-ATPase and F_0_1-ATP synthase." FEBS Lett. 359. 69-72 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Maeda: "The intrinsic factor gene:Its 5′-upstream region and chief cell-specific trnscription." J.Biochem.117. 1305-1311 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Nishi: "Identification of the promoter region of the human histamine H2 receptorgene." Biochem.Biophys.Res.Commum.210. 616-623 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshida: "A common topology of proteins catalyzing ATP-triggered reactions." FEBS Lett.359. 1-5 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Miyauchi: "F_1ATPase α-subunit made up from two fragments(1-395,396-503)is stabilized by ATP and complexes containing it obey altered kinetics." Biochim.Biophys.Acta. 1229. 225-232 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Saika: "Aminimum catalytic unit of F_1-ATPase shows non-cooperative ATPase activity inherent in a single catalytic site with a K_m70μM." FEBS Lett.368. 207-210 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Matsui: "Expression of the wild-type andthe Cys-/Trp-less α3β3γ complex of thermipholic F_1-ATPase in Escherichia coli." Biochim.Biohys.Acta. 1231. 139-146 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Kato: "Analysis of time dependent change of Escherichia ColiF_1-ATPase activity and its relationship with apparent negative cooperativity." Biochim.Biophys.Acta. 1231. 275-281 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Tozawa: "Conformational dynamics monitored by His-179 and His-200 of isolated thermophilic F_1-ATPase β subunit which rerside at the entrance of′conical tunnel′ in holoenzyme." FEBS Lett.376. 190-194 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] C.Kaibara: "Strutural asymmetry of F_1-ATPase caused by the γ subunit generates a high affinity nucleotide binding site" J.Biol.Chem.271(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Fukuyama: "Tertiary structure of [2Fe-2S]ferredoxin from Spirulina platensis refined at 2.5Å resolution:Structural comparison of plant-type ferredoxins and electrostatic potential analysis." J.Biochem.117. 1017-1023 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Oh-oka: "Two molecules of cytochrome c function as the electron donors to P840 in the reaction center complex isolated from a green bacterium,Chlorobium tepium." FEBS Lett.365. 30-34 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.oh-oka: "Photoactive reaction center(PscA/cytochrome c_<551>)_2complex of Chlorobium limicola." Proc.Xth Int.Photosynthesis Congress. (印刷中). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 南 善子: "広く生体反応に関わる鉄硫黄蛋白質" 生物物理. 35. 7-12 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Ono: "Conformational aberrance of Sendai virus F_0 protein in thapsigargin-treated cells allowing exit from the endoplasmic reticulum but causing arrest at the Golgi complex." J.Biochem.118. 1248-1254 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Sugimoto: "Significance of urinary N1,N8-diacetylspermidine and N1,N12-diacetylspermine as indicators of neoplastic diseases." J.Cancer Res.Clin.Oncol.121. 317-319 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Suzuki: "Sites of labeling with N-(3-pyrene)maleimide on Ca^<2+>-transporting ATPase of the sarcoplasmic reticulum." J.Biochem.117. 881-887 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Hiramatsu: "Determination of amounts of polyamines excreted in urine:demonstration of N1,N8-diacetylspemidine and N1,N12-diacetylspermine as components commonly occurring in normal." J.Biochem.117. 107-112 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Okajima: "Cloning,sequencing,and expression in Escherichia coli cDNA encoding porcine brain UMP-CMPkinase." J.Biochem.117. 980-986 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Fukui: "Exploring the uncleotide-binding site in proteins by affinity labeling and site-directed mutagenesis" J.Biochem.117. 1139-1144 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Kagawa: "Gene of heat shock protein of sulfur-dependent archaeal hyperthermophile Desulfurcoccus." Biochem.Biophys.Res.Commun.214. 730-736 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Sato: "ATP-hydrolyzing excitation state of the reconstituted α3β3 complex of ATP synthase from the thermphilic bacterium PS3:Structural chracteristics shown by time-resolved small-angle X-rayscattering with synchrotron radiation." J.Biochem.117. 113-119 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Endo: "A candidate gene for RNA export carrier protein has two RBDs(RNA binding domain and Ran binding domain)." Nucleic acid Symp.Series. 33. 30-32 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] D.Takai: "Age-associated changes of mitochondrial translation and respiratory function in mouse brain." Biochem.Biophys.Res.Commun.217(印刷中). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Akiyama: "Energetics and formation of ATP synthase:Allosteric,chemiosmotic and biosynthetic control of αβ and αβγ core." Biochemistry of Cell Membrane(eds.S.Papa and J.M.Tager)Birkhauser Verlag,Basel,Swiss. 167-178 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Endo: "Heart/muscle-specific alternative splicing in ATP synthase γ-subunit pre-mRNA during muscle development." Cardiac Development and Gene Regulation(ed.Y.Tazaki),Excepta Medica. 77-97 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小澤高将: "遺伝子医学:ミトコンドリア病" 第24回日本医学会会誌. 1. 207-213 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Orii: "Facilitated intramolecular electron transfer in the Escherichia coli bo-type ubiquinol oxidase requires chloride." Biochemistry. 34. 1127-1132 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Mogi: "CuB promotes both binding and reduction of dioxgen at the heme-copper binuclear center in the Escherichia coli bo-type ubiquinol oxidase." FEBS Lett.370. 259-263 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Sato-Watanade: "Stabilization of a semiquinone redical at the high affinity quinone binding site of the Escherichia coli bo-type ubiquinol oxidase." FEBS Lett.374. 265-269 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Hirota: "Resonance Raman study on axial ligands of heme irons in cytochrome bd-type ubiquinol oxidase from Escherichia coli." Biospectroscopy. 1. 305-311 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Tsubaki: "Cyanide-binding site of bd-type ubiquinol oxidase from Escherichia coli." J.Biol.Chem.270. 28565-28569 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Denda: "Characterization of chimeric heme-copper respiratory oxidases using subunits I of Escherichia coli cytochrome bo and Halobacterium salinarium cytochrome aa_3." Biochem.Biophys.Res.Commun.217. 428-436 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Tsubaki: "FT-IR and EPR studies on cyanide-bindings to the heme-copper binuclear center of cytochrome bo-type ubiquinol oxidase from Escherichia coli:Release of CuB-cyano complex in the partially reduced states." J.Biol.Chem.271(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Moriyama: "Localization of N-methylmaleimide-sensitive fusion protein in pinealocytes" Neuro.Report. 6. 1757-1760 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Tsukahara: "A subpopulation of large ganglion neurons express Isk protein mRNA:An in situ hybridization analysis in the rat eye." Mol Brain Res.29. 376-380 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Nakai: "Expression and phosphorylation of BiP/GRP78,a molecular chaperone in the endoplasmic reticulum,during the Differentiation of a mouse myeloblastic cell line." Cell Struct.Func.20. 33-39 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 山本章嗣: "ライソゾームの機能とV-ATPase." 生体の科学. 44. 262-266 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Miyai: "Na^+/myo-inositol cotransport is regulated by tonicity in cultured rat mesangial cells." Kidney Int.47. 473-480 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.-S.Chen: "Ethanol induces heterogeneous reduction of cytochrome a-3 within perfused rat liver lobule assessed using microspectroscopy." Alcohol and Alcoholism. 30. 187-194 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Kaneko: "Activation of Na^+/H^+exchanger by hepatocyte growth factor in hepatoctes." Hepatology. 22. 629-636 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.-J.Schaefer: "2,8-Diazo-ATP,a short-length bifunctional photoaffinity label for photoaffinity cross-linking of a stable F_1 in ATP synthase(from thermophilic bacteria PS3)." FEBS Lett.377. 408-412 (1995)

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      1995 Annual Research Report
  • [Publications] 香川靖雄: "老化のバイオサイエンス" 羊土社(印刷中), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 宇井理生: "生体膜-新しい膜学への招待" 講談社, 298 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Mineuki: "Loss of microtubles in the interphase cells of onion (Allium cepa L.) root tips from the cell cortex and their appearance in the cytoplasm after treatment with cycloheximide." Plant Physiol.104. 281-284 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.Nakajima-Shimada: "Determination of intracellular Calcium concentration of individual host cells infected with Trypanosoma cruzi using microfluorimetry in conjunction with digital image processing." Jpn. J. Parasitol.43. 136-139 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Anraku: "Protozyme: Emerging evidence in nature." J. Biochem.115. 175-178 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] I.Yamato: "Site-specific alteration of Arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli." J. Biol. Chem.269. 5720-5724 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Yoshida: "Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae." Mol. Gen. Genet.242. 631-640 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Qadota: "Conditional lethaliy of a yeast strain expressing human RHOA in place of RHO1." Proc. Natl. Acad. Sci. USA. 91. 9317-9321 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Takita: "The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca^<2+> tolerance in yeast." Mol. Gen. Genet.(in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Moriyana: "ATP-dependent uptake anti-neoplastic agents by acidic organelles." J. Biochem.115. 213-218 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Mogi: "Biosynthesis and functional role of heme O and heme A." Mol. Microbiol.14. 391-398 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Mogi: "Molecular structure of a heme-copper redox center of the Escherichia coli ubiquinol oxidase: Evidence and model" J. Biochem.116. 741-747 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M. Sato-Watanabe: "Structure-function studies on the ubiquinol oxidation site of the cytochrome bo complex from Escherichia coli using p-benzoquinones and substituted phenols." J. Biol. Chem.269. 28899-28907 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Sato-Watanabe: "Identification of a novel quinone binding site in the cytochrome bo complex from Escherichia coli." J. Biol. Chem.269. 28908-28912 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Tsubaki: "Molecular structure of redox metal centers of the cytochrome bo complex from Escherichia coli: Spectroscopic characterizations of the subunit I histidine mutant oxidases." J. Biol. Chem.(in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Saiki: "An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase." FEBS Lett.351. 385-388 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Hirota: "Observation of the Fe-O_2 and Fe^<IV>=O Raman bands in dioxygen reduction by cytochrome bo-type ubiquinol oxidase from Escherichia coli." FEBS Lett.352. 67-70 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Orii: "Facilitated intra-molecular electron transfer in the cytochrome bo-type ubiquinol oxidase initiated upon reaction of the fully-reduced enzyme with dioxygen." FEBS Lett.352. 151-154 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Maeda: "Gastric proton pump (H^+/K^+-ATPase): Structure and gene regulation through GATA DNA-binding protein(s)." J. Biochem.115. 6-14 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Omote: "The α/β subunit interaction in H^+-ATPase (ATP synthase): An Eschericia coli α subunit mutation (αArg-296→Cys) restored coupling efficiency to the deleterious β subunit mutant (βSer-174→Phe)." J. Biol. Chem.269. 10265-10269 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Mushiake: "Genes for members of the GATA-binding protein family (GATA-GT1 and GATA-GT2) together with H^+K^+-ATPase are specifically transcribed in gastric parietal cells." FEBS Lett.340. 117-120 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] R.K.Nakamoto: "The F_oF_1 ATP synthase: Structure involved in catalysis, transport and coupling." Biomembranes. 6(in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Futai: "Catalysis and energy coupling ofH^+-ATPase(ATP synthase): Molecular biological approaches." Biochim. Biophys. Acta. 1187. 165-170 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.-Y. Park: "Conserved Glu-181 and Arg-182 residues of Escherichia coli H^+-ATPase (ATP synthase) β subunit are essential for catalysis: Properties of 33 mutants between βGlu-161 and βLys-201 residues." J. Biochem.116. 1139-1145 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Iwamoto: "N-Ethlmaleimide-sensitive mutant (βVal-153→Cys) Escherichia coli F_1 ATPase: Cross-linking of the mutant β subunit with the α Subunit." FEBS Lett.352. 243-246 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Moriyama: "Quinnacrine mustard and hydrophobic amines both vacuolar H^+-ATPase and F_oF_1 ATP synthase." Biochim. Biophys. Res. Commun.(in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Uno: "Resonance Raman and Fourier-transform infrared studies on the subunit I histidine mutants of the cytochrome bo complex in Escherichia coli: Molecular structure of redox centers." J. Biol. Chem.269. 11912-11920 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Futai: "H^+-ATPase: Catalysis and H^+ translocation." Molecular Aspects of Enzyme Catalysis. Kodansha 2nd/VCH Publishers, N.Y..(in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Yuzaki: "A stimulus paradigm inducing long-term desensitization of AMPA receptors evokes a specific increase in BDNF mRNA in cerebellar slices." Learning and Memory,. (in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Akiyama: "Energetics and formation of ATP synthase: Allosteric, chemiosmotic, and biosynthetic control of αβ and αβγ core." Biochemistry of Cell Membranes (Papa, S.and Tager, J.M.,eds.) Birkhauser, Basel, Switzerland,. (in press.).

    • Related Report
      1994 Annual Research Report
  • [Publications] 二井将光: "オスモエンザイム, H^+-ATP合成酵素‥反応と輸送‥" 蛋白質核酸酵素. 39. 1141-1151 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "パーティクル銃による遺伝子導入‥ミトコンドリア遺伝子治療とP^o株細胞" 実験医学増刊「遺伝子治療の最前線」. 12. 1835-1840 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "インスリンと糖代謝の分子生物学." 病態生理. 13. 1-11 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "ミトコンドリアの分子遺伝学と糖尿病." 日本臨床. 52. 85-91 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Kawamoto: "New crystal forms and preliminary X-ray diffraction studies of mitochondrial cytochrome bc_1 complex from bovine heart." J.Mol.Biol.244. 238-241 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Miyazaki: "Crystallization and preliminary X-ray diffraction studies of the α-amylase inhibitor coded 0.19 from wheat kernel." J.Biochem.115. 178-181 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Kunishima: "Crystal structure of the fungal peroxidas from Arthromyces ramosus at 1.9Å resolution. Structural comparisons with the lignin and cytochrome c peroxidases." J.Mol.Biol.235. 331-344 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.-Y.Cui: "Molecular cloning and nucleotide sequences of cDNAs encoding subunits I, II, and IX of Euglena gracilis mitochondrial complex III." J.Biochem.115. 98-107 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Ono: "Transport of envelope proteins of Sendai virus, HN and F_o, is blocked at different steps by thapsigargin and other perturbants to intracellular Ca^<2+>." J.Biochem.116. 649-656 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Suzuki: "Sites of labeling with N-(3-pyrene)maleimide on Ca^<2+>-transporting ATPase of the sarcoplasmic reticulum." J.Biochem.117(in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.-I. Hayashi: "Functional and morphological abnormalities of mitochondria in human cells containing mitochondrial DNA with pathogenic point mutations in tRNA genes." J.Biol.Chem.269. 19060-19066 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.-I. Hayashi: "Nuclear but not mitochondrial genome involvement in human age-related mitochondrial dysfunction. Functional integrity of mitochondrial DNA from aged subjects." J. Biol. Chem.269. 6878-6883 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Endo: "Exclusion of an alternatively spliced exon in human ATP synthase γ-subunit pre-mRNA requires de novo protein synthesis." J.Biol.Chem.269. 12488-12493 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Burgard: "Effects of magnesium ions on the relative conformation of nucleotide binding sites of F_1-ATPase as studied by ESR-spectroscopy." J.Biol.Chem.269. 17815-17819 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Nakanishi: "Purification and reconstitution of an intestinal Na^+-dependent neutral L-α-amino acid transporter." J.Biol.Chem.269. 9325-9329 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Akiyama: "Gene structure and cell type-specific expression of the human ATP α subunit." Biochim.Biophys.Acta. 1219. 129-140 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Schuhen: "Photoaffinity cross-linking of ATP synthase complexes by fluorescent bifunctional diadenine-dinucleotide." Biol.Chem.Hoppe-Seyler. 375. S64 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Koike: "Transcription of the MRP RNA gene in frog I oocytes requires a novel cis-element." Biochem.Biophys.Res.Commun.202. 225-233 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Knoche: "2-Azido-1,N6-etheno-ATP: A fluorescent photoaffinity label for ATP synthase complexes." Biol.Chem.Hoppe Seyler. 375. S64 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] C.Matsuda: "Comparison of the ATPase activities of bovine heart and liver mitochondrial ATP synthase with different tissue-specific γ- subunit isoforms." Biochem.Biophys.Res.Commun.200. 671-678 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "エネルギー代謝制御における糖質の意義." NANO GIGA. 3. 54-60 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "パーティクル銃とP^O細胞の融合技術." 細胞工学. 13. 830-834 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 富永薫: "核遺伝子によるミトコンドリアDNAの発現調節." 臨床科学. 130. 453-463 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "分子シャペロンとその誘導." 蛋白質核酸酵素. 39. 808-817 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "ミトコンドリア異常による糖尿病." Isotope News. 484. 5 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "分子シャペロンとその誘導." 蛋白質核酸酵素. 39. 791-792 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 香川靖雄: "ストレスタンパク質と細胞小器官." 細胞工学. 13. 169-175 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Ueno: "Functional consequences of the substitution of disulfide-bonded segment, Cys127-Cys150, located in the extracellular domain of the Na, K-ATPase β subunit: Arg148 is essential for hte functional expression of Na, K-ATPase." J.Biochem.(in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Yokoyama: "Isolation of prokaryotic V_oV_1-ATPase from a thermophilic eubacterium Thermus thermophilus." J.Biol.Chem.269. 12248-12253 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Okada: "Tyr-341 of the β subunit is a major Km-determining residue of TF_1-ATPase; Parallel effect of its mutations on Kd(ATP) of the β subunit and on the Km of the α_3β_3γ complex." J.Biochem.115. 789-796 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Saika: "Crystallization of the mutant β subunit of F_1-ATPase from thermophilic Bacillus PS3." J.Mol.Biol.236. 709-711 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Hisabori: "Asymmetry of the three catalytic sites on β subunits of TF_1 from a thermophilic Bacillus PS3." J.Biochem.115. 497-501 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.-M. Jault: "Probing the specificity of nucleotide binding to the F_1-ATPase from thermophiic Bacillus PS3 and its isolated α and β subunits with 2-N_3-[β,γ-^<32>P]ATP." Arch.Biochem.Biophis.310. 282-288 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Amano: "Spatial precision of a catalytic carboxylate of F_1-ATPase β subunit probed by introducing different carboxylate containing side chains." FEBS Lett.348. 93-98 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Amano: "Structural model of the ATP-binding domain of the F_1-β subunit based on analogy to the RecA protein." FEBS Lett.351. 1-5 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Kawashima: "Widespread tissue distribution of multiple mitochondrial DNA deletions in familial mitochondrial myopathy." Muscle Nerve. 17. 741-746 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Kagawa: "Mitochondrial α-keto acid dehydrogenase complexes." Jp.Soc.Prom.Sci.,Tokyo. pp.1-37 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Tanaka: "Characterization of signaling pathways to Na^+/H^+ exchanger acivation with epidermal growth factor in hepatocytes." Hepatology. 20. 966-974 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Kusumi: "Regulation of PEP-carboxylase by biological clock in a CAM plant." Plant Cell Physiol.35. 233-242 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Kusumi: "Chlorophyll deficiency caused by a specific blockage of the C_5-pathway in seedlings of virescent mutant rice." Plant Cell Physiol.35. 445-449 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Kodama: "Genetic enhancement of cold tolerance by expression of a gene for chloroplast ω-3 fatty acid desaturase in transgenic tobacco." Plant Physiol.105. 601-605 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Hamada: "Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase." Gene. 147. 293-294 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Nishiuchi: "Genomic nucleotide sequence of a gene encoding a microsomal ω-3 fatty acid desaturase from Arabidopsis thaliana." Plant Physiol.105. 767-768 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] R.M.Hong: "Association of N-ethylmaleimide-sensitive factor with synaptic vesicles." FEBS Lett.350. 253-257 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Mukohata: "Comparative studies on ion pumps of the bacterial rhodopsin family." Biophys.Chem.50. 191-201 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] E.P.Lukashev: "pH dependence of the absorption spectra and photochemical transformations of the archaerhodopsins." Photochem.Photobiol.60. 69-75 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Sugiyama: "Archae-opsin expressed in Escherichia coli and its conversion to purple pigment." J.Biochem.115. 1021-1026 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Ihara: "Met-145 is a key residue in the dark adaptation of bacteriorhodopsin homologs." Biophys.J.67. 1187-1191 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Tateno: "The novel ion pump rhodopsin from Haloarcula form a family independent from both the bacteriorhodopsin and archaerhodopsin families/tribes." Arch.Biochem.Biophys.315. 127-132 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Sugiyama: "The light-driven proton pump, cruxrhodopsin-2 from Haloarcula sp.arg-2 (bR^+hR^-), and its coupled ATP formation." Biochim.Biophys.Acta. (in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Orii: "Facilitated intramolecular electron transfer in the Escherichia coli bo-type ubiquinol oxidase requires chloride." Biochemistry. (in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Furukawa: "Identificatioh of Lys-277 at the active site of Escherichia coli glycogen synthase: Application of affinity labeling combined with site-directed mutagenesis." J.Biol.Chem.269. 868-871 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Kaya: "Pyridoxal 5'-phosphate probes at Lys-480 can sense the binding of ATP and the formation of phosphoenzymes in Na^+,K^+-ATPase." J.Biol.Chem.269. 7419-7422 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] C.Hountondji: "Affinity labeling of the two species of Escherichia coli Lysyl-tRNA synthetase with Adenosine di- and triphosphopyridoxals." J.Biochem.116. 493-501 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] C.Hountondji: "Affinity labeling of the two species of Escherichia coli Lysyl-tRNA synthetase with pyridoxal mono- and diphosphophyridoxals." J.Biochem.116. 502-507 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.Deguchi: "Acidification of phagosomes and degradation of rod outer segments in rat retinal pigment epithelium." Invest.Ophthalomol.Vis.Sci.35. 568-579 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Sugasawa: "Immunocytochemical analyses of distribution of Na, K-ATPase and GLUTI, insulin and transferrin receptors in developing retinal pigment epithelial cells." Cell Struct.Funct.19. 21-28 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] G.Kawa: "Sodium pump distribution is not reversed in the DBA/2FG-pcy, polycystic kidney disease model mouse." J.Am.Soc.Nephrol.4. 2040-2049 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Yamamoto: "Immunocytochemical localization of Na, K-ATPase in rat muscle spindles." Cell Struct.Funct.19. 179-187 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Anraku: "Protoenzyme:Emerging evidence in nature." J.Biochem.115. 175-178 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.N.Ho: "VMA13 encodes a 54-kDa vacuolar H^+-ATPase subunit required for activity but not assembly of the enzyme complex in Saccharomyces cerevisiae." J.Biol.Chem.268. 18286-18292 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] R.Hirata: "VMA12 is essential for assembly of the vacuolar H^+-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae." J.Biol.Chem.268. 961-967 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Ishii: "Three dimensional structure of F_1-ATPase of thermophilic bacterium PS3 obtained from electron crystallography." J.Biochem.113. 245-250 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] C.Kaibara: "AT(D)P Mg-induced dissociation of the α_3β_3 complex of the F_1-ATPase from a thermophilic Bacillus PS3 into α_1β_1 heterodimers is prevented by mutation β(Y341C)." FEBS Lett.321. 46-50 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Tozawa: "Structure of the α subunit of F_1-ATPase probed by limited proteolysis." J.Biol.Chem.268. 19044-19054 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] E.Muneyuki: "Inhibitory effect of NaN_3 on the F_0F_1 ATPase of submitochondrial particles as related to nucleotide binding." Biochim.Biophys.Acta. 1144. 62-68 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] E.Muneyuki: "Direct transmembraneous reconstitution of bacteriorhodopsin into planar phospholipid bilayers." Biochim.Biophys.Acta. 1183. 171-179 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Odaka: "In vitro affinity label of a protein expressed in Escherichia coli;Coenzyme A occupied the AT(D)P binding site of the mutant F_1-ATPase β subunit(Y307C)through a disulfide bond." FEBS Lett.336. 231-235 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Iwamoto: "Domains near ATP γ phosphate in the catalytic site of H^+-ATPase:Model proposed from mutagenesis and inhibitor studies." J.Biol.Chem.268. 3156-3160 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] R.K.Nakamoto: "The γ subunit of the Escherichia coli ATP synthase:Mutations in the carboxyl-terminal region restore energy coupling to the amino-terminal mutant γ Met-23→Lys." J.Biol.Chem.268. 867-872 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] D.Xie: "The atp2 operon of the green bacterium Chlorobium limicola." Biochim.Biophys.Acta. 1172. 267-273 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nakai: "Acidic regions of cytochrome c_1 are essential for ubiquinol-cytochrome c reductase activity in yeast cells lacking the acidic QCR6 protein." J.Biochem.114. 919-925 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nakai: "Intramitochondrial protein sorting:Isolation and characterization of the yeast MSP1 gene which belongs to a novel family of putative ATPases." J.Biol.Chem.268. 24262-24269 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] J.-Y.Cui: "Molecular cloning and nucleotide sequences of cDNAs encoding subunits I,II,and IX of Euglena gracilis mitochondrial complex III." J.Biochem.115. 98-107 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Oh-oka: "Isolation of the photoactive reaction center complex that contains three types of Fe-S centers and a cytochrome c subunit from the green sulfur bacterium Chlorobium limicola f.thiosulfatophilum,strain Larsen." Plant Cell Physiol.34. 93-101 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Kunishima: "Crystallization and preliminary X-ray diffraction studies of peroxidase from a fungus Arthromyces ramosus." Proteins,Structures,Functions,and Genetics. 15. 216-220 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Saeki: "Nucleotide sequence and genetic analysis of the region essential for functional expression of the gene for ferredoxin I,fdxN,in Rhodobacter capsulatus:Sharing of one upstream activator sequence in opposite directions by two operons related to nitrogen fixation." Plant Cell Physiol.34. 185-199 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Fujita: "Identification of a nifDK-like gene(ORF467)involved in the biosynthesis of chlorophyll in the cyanobacterium Plectonema boryanum." Plant Cell Physiol.34. 305-314 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Yamashita: "Characterization of the Na^+/H^+-antiporter of bovine renal cortex and its immunoaffinity purification in a reconstitutively active form." J.Biochem.113. 136-140 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Suzuki: "Uncoupling of ATP splitting from Ca^<2+>-transport in Ca^<2+>-transporting ATPase of the sarcoplasmic reticulum as a result of modification by N-(3-pyrene)maleimide:Activation of a channel with a specificity for alkaline earth metal ions." J.Biochem.114. 203-209 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Kuroda: "Calcium pump expression in human bone and soft tissue tumors." Virchows Archiv.A Pathol.Anat.423. 209-213 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Hara: "The UDP-galactose translocator gene is mapped to the Wiskott-Aldrich syndrome locus of the X chromosome." Somat.Cell Mol.Genet.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] C.Matsuda: "Gene structure of human mitochondrial ATP synthase γ-subunit:Tissue-specificity produced by alternative RNA splicing." J.Biol.Chem.268. 24950-24958 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Takiyama: "The gene for Machado-Joseph disease maps to human chromosome 14q." Nature Genetics. 4. 300-304 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Ohta: "Heat shock promoter of thermophilic chaperonin operon." Biochem.Biophys.Res.Commun.191. 550-557 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Sunada: "Inherited amyloid polyneuropathy type IV(gelsolin variant)in a Japanese family." Ann.Neurol.33. 57-62 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nakanishi: "Solubilization and reconstitution of high-and low-affinity Na^+-dependent neutral L-α-amino acid transporters from rabbit small intestine." Biochim.Biophys.Acta. 1151. 193-200 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] C.Matsuda: "Tissue-specific isoforms of the bovine mitochondrial ATP synthase γ-subunit." FEBS Lett.325. 281-284 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.-J.Schaefer: "Photocrosslinking of ATP synthase." Biol.Chem.Hoppe-Seyler. 674. 164 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Yuzaki: "Cerebellar glia specifically supports the survival of Purkinje cells in culture." Biochem.Biophys.Res.Commun.197. 123-129 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Tominaga: "Smaller isoform of human mitochondrial transcription factor 1:Its wide distribution and production by alternative splicing." Biochem.Biophys.Res.Commun.194. 544-551 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Schuhen: "Fluorescent bifunctional nucleotide analogs." Biol.Chem.Hoppe-Seyler. 674. 164 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Shiraiwa: "Content of mutant mitochondrial DNA and organ-dysfunction in a patient with a MELAS-subgroup of mitochondrial encephalomyopathies." J.Neurol.Sci.in press.

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nakanishi: "Purification and reconstitution of an intestinal Na^+-dependent neutral L-α-amino acid transporter." J.Biol.Chem.269(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] J-I.Hayashi: "Nuclear but not mitochondrial genome involvement in human age-relateed mitochondrial dysfunction:Funcional integrity of mitochondrial DNA from aged subjects." J.Biol.Chem.269(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.S.Chen: "Measurement of redox states of mitochondrial cytochrome aa_3 in regions of liver loble by reflectance spectroscopy." Am.J.Physiol.264. G375-382 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Kinoshita: "Phosphorylation and dephosphorylation of guard-cell proteins from Vicia faba L.in response to light and dark." Plant Physiol.102. 917-923 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Fuse: "Characterization of a rice mutant having an increased susceptibility to light stress at high temperature." Physiol.Plantarum. 89. 799-804 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Iba: "A gene encoding a chloroplast ω-3 fatty acid desaturase complements alterations in fatty acid desaturataion and chloroplast copy number of the fad7 mutant of Arabidopsis thaliana." J.Biol.Chem.268. 24099-24105 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Kusumi: "Chlorophyll deficiency caused by a specific blocking of the C_5-pathway in virescent mutant seedlings of rice." Plant Cell Physiol.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Kusumi: "Regulation of PEP-carboxylase by biological clock in a CAM plant." Plant Cell Physiol.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Henomatsu: "Inhibition of intracellular transport of newly synthesized prolactin 6 by bafilomycin A1 in a pituitary tumor cell line,GH_3 cells." Eur.J.Cell Biol.62. 127-139 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Manabe: "Inhibitors of vacuolar-type H^+-ATPase suppresses proliferation of cultured cells." J.Cell Physiol.157. 445-452 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] J.Deguchi: "Acidification of phagosomes and degradation of rod outer segments in rat retinal pigment epithelium." J.Ophotamol.Vis.Sci.35(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Moriyama: "ATP-dependent uptake of anti-neoplastic agents by acidic organelles." J.Biochem.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Okajima: "Site-directed random mutagenesis of AMP-binding residues in adenylate kinase." J.Biochem.114. 627-633 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Okajima: "Site-directed mutagenesis of AMP-binding residues in adenylate kinase:Alteration of substrate specificity." FEBS Lett.334. 86-88 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Kaya: "Pyridoxal-5'-phosphate probes at Lys-480 can sense the binding of ATP and the formation of phosphoenzymes in Na^+,K^+-ATPase." J.Biol.Chem.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Mukohata: "Comparative studies on ion pumps of the bacteriorhodopsin family." Biophys.Chem.48(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Ihara: "Met-145 is a key residue in the light-dark adaptation of bacterial rhodopsins." Biophys.J.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Sugiyama: "Archaeopsin expressed in E.coli and its convertion to purple pigment in vitro." J.Biochem.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Tateno: "The proton pump rhodopsins from Haloarcula differ from bacteriorhodopsin and archaerhodopsins." Arch.Biochem.Biophys.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Saiki: "Identification of the functional domain in heme O synthase:Site-directed mutagenesis studies on the cyoE gene of the cytochrome bo operon in Escherichia coli." J.Biol.Chem.268. 26927-26934 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Saiki: "In vitro heme O synthesis by the cyoE gene product from Escherichia coli." J.Biol.Chem.268. 26041-26045 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Tsubaki: "Structure of the heme-copper binuclear center of the cytochrome bo complex of Escherichia coli:EPR and Fourier transform infrared spectroscopic studies." Biochemistry. 32. 6065-6072 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Tsubaki: "Cytochrome daxial ligand of the bd-type terminal quinol oxidase from Escherichia coli." FEBS Lett.335. 13-17 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Uno: "Resonance Raman and Fourier-transform infrared studies on the subunit I histidine mutants of the cytochrome bo complex in Escherichia coli:Molecular structure of redox metal centers." J.Biol.Chem.269(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.N.Zhang: "Effects of mutagenic substitutions of prolines on the rate of deprotonation and reprotonation of the Schiff base during the photocycle of bacteriorhodopsin." Photochem.Photobiol.57. 1027-1031 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 佐伯和彦: "フェレドキシンの進化、はじめに" 新生化学実験講座 分子進化実験法(東京化学同人). 16. 153-154 (1993)

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      1993 Annual Research Report
  • [Publications] 香川靖雄: "酸化的リン酸化" 生体の科学 特集「現代医学、生物学の仮説、学説」(医学書院). 44. 410-411 (1993)

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      1993 Annual Research Report
  • [Publications] 香川靖雄: "細胞の一般生理" 標準生理学第3版(医学書院). 7-41 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 吉森保: "小胞輸送と選別の分子機構" 蛋白質核酸酵素 特集「分泌系蛋白質輸送の分子機構」. 38. 927-938 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森山芳則: "液胞型H^+-ATPase阻害剤としてのバフィロマイシンおよび類縁化合物" 蛋白質核酸酵素 特集「細胞機能研究のための低分子プローブ」. 38. 2000-2011 (1993)

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      1993 Annual Research Report
  • [Publications] T.Fukui: "Molecular aspects of enzyme catalysis" Kodansha Ltd./VCH Verlags AG, 241 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 香川靖雄: "生体膜と疾患の分子生物学" 南山堂, 560 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 香川靖雄: "図説医化学第2版" 南山堂, 486 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 香川靖雄: "エネルギー、生命、環境そして栄養" 女子栄養大学出版部, 280 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Anraku: "Genetic and cell biological aspects of the yeast vacuolar H^+-ATPase." J.Bioenerg.Biomembr.24. 395-405 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Anraku: "Molecular genetics of the yeast vacuolar H^+-ATPase." J.Exp.Biol.172. 67-81 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Wada: "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.1.Isolation anmd characterzation of two classes of vam mutants." J.Biol.Chem.267. 18665-18670 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Wada: "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.2.VAM7,a gene for regulating morphogenic assembly of the vacuoes." J.Biol.Chem.267. 18671-18675 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Wada: "Chloride transport of yeast vacuolar membrane vesicles: A study of in vacuolar acidification." Biochim.Biophys.Acta. 1101. 296-302 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.Hirata: "Mutations at the putative junction sites of the yeast VMA1 protein,the catalytic subunit of the vacuoal membrane H^+-ATPase,inhibit its processing by protein splicing." Biochem.Biophys.Res.Commun.188. 40-47 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Hisabori: "Single site hydrolysis of TNP-ATP by F_1-ATPase from the thermophilic bacterium PS3 is accelerated by chase-addition of excess ATP." J.Biol.Chem.267. 4551-4556 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] D.Bar-Zvi: "Covalent binding of 3'-0-(4-Benzoyl)benzoyl adenosine 5'-triphosphate (BzATP)to the isolated α and β subunits and α_3β_3 core complex of TF_1." J.Biol.Chem.267. 11029-11033 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Tozawa: "Molecular dissection of the β subunit of F_1-ATPase into peptide fragments." J.Biol.Chem.267. 16484-16490 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Sumi: "A DNA fragment homologous to F^1-ATPase β subunit was amplified from genomic DNA of Methanosarsina barkeri: Indication of an archaebacterial F-type ATPase." FEBS Lett.314. 207-210 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] N.Ishii.: "Three dimentional structure of F^1-ATPase of thermophilic bacterium PS3 obtained from electron crystallography." J.Biochem.113. 245-250 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] M,Jounouchi: "Catalytic site in the β subunit of H^+-ATPase from Escherichia coli:Glycine-rich sequence and its interaction weth Ser-174." Arch.Biochem.Biophys.292. 376-381 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M,Jounouchi: "Role of the amino terminal region of the ε subunit of Escherichia coli H^+-ATPase(F_0F_1)." Arch.Biochem.Biophys.292. 87-94 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Futai: "Catalytic site the β subunit of H^+-ATPase from Escherichia coli: Glycine-rich sequence and its interaction with Ser-174." Acta Physiol.Scan.146. 125-130 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.Miki: "Mapping of antigenic sites to monoclonal antibodies on the primary structure of the F_1-ATPase β subunit from Escherichia coli: Conserved amino-terminal region of the subunit in the F_1." Arch.Biochem.Biophys.294. 373-381 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Noguchi: "Assembly of a hybrid from the α subunit Na^+/K^+-ATPase and β subunit of H^+/K^+-ATPase and β subunit of H^+/K^+-ATPase." Biochem.Biophys.Res.Commun.182. 659-666 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Hasebe: "Vacuolar type H^+-ATPase genes:Presence of four genes including pseudogenes for the 16 kDa proteolipid subunit in the human genome." Biochem.Biophys.Res.Commyn.183. 659-666 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Tamura: "Sequence motif in control region of the H^+/K^+-ATPase a and β subunit genes recognized by gastric specific nuclear protein." FEBS Lett.298. 137-141 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Moriyama: "Involvement of a non-proton pump factor (possibly Donnan-type equilibrium)in maintenance of an acidic pH in lysosomes." FEBS Lett.302. 18-20 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Futai: "A glycine-rich sequence in the catalytic site of F-type ATPase." J.Biomembr.and Bioenerg.24. 463-467 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Moriyqma: "Role of vacuolar-type H^+-ATPase in neuronal and endocrine systems." J.Exp.Biol.172. 171-178 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Futai: "Escherichia coli ATP synthase (F-type ATPase):Catalytic site and regulation of H^+ translocation." J.Exp.Biol.172. 443-449 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Omote: "Effects mutations of conserved Lys-155 and Thr-156 residues in the phosphate binding glycine-rich sequence of the F_1-ATPase β subunit of Escherichia coli." J.Biol.Chem.267. 20571-20576 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Shin: "F_0F_1-ATPase γ subunit mutations perturb the coupling between catalysis and transport" J.Biol.Chem.267. 20835-20839 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.K.Nakamoto: "Escherichia coli F_0F_1-ATPase:Residues involved in catalysis and coupling." Anal.N.Y.Acad.Sci.(1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.K.Nakamoto: "The γ subunit of the Escherichia coli ATP synthase:Mutations in the carboxyl-terminal region restore energy coupling to the amino-terminal mutant γ Met-23->Lys." Anal.N.Y.Acad.Sci.268. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] A.Iwamoto: "Domains near ATP γ phosphate in the catalytic site of H^+-ATPase:Model proposed from mutagenesis and inhibitor studies" J.Bl.N.Y.Acad.Sci.268. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] X.Wang: "Characterization of human autoantibodies reactibe to gastric parietal cells" Biochem.Biophys.Res.Commun.190. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Yoshikawa: "Stategies for ctystallization of large membrane protein complexes." J.Crystal Growth. 122. 298-302 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Yamashita: "Reconstitution of Na^+/H^+-antiporter of bovine renal brush-border membrane into proterliposomes and detection of a 110kDa protein cross- reactive with antibodies against a human Na^+/H^+-antiporter partial peptide in antiporter-active fractions after partial fractionation." J.Biochem.111. 162-167 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Kuroda: "Immunohistochemical assessement of sarcoplasmic reticulum Ca^<2+>-ATPase distribution in various human tissues using novel monoclonal antibodies." Virchows Archiv A:Pathol. Anat.Histophthol.421. 527-532 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Yamashita: "Characterization of Na^+/H^+-antipoter of bovine renal cortex and its immunoaffinity purification in reconstitutively actibe form." J.Biochem.113(2). (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Kagawa: "The α_3β_3 and α_1β_1 complexes of ATP synthase." Ann.New York Acad.Sci.671. 366-376 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Kamata: "Primary structure of the alanine carrier protein of thermophilic bacterium PS3." J.Biol.Chem.267. 21650-21655 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Kagawa: "The αβ complexes of ATP synthase:The αα_3β_3oligomer and α_1β_1 protomer." J.Bioenerg.Biomembr.24. 441-445 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Kobayashi: "The mutant mitochondrial genes in mitochondrial myopathy,encephalopathy,lactic acidosis and stroke-ike episodes (MELAS)were selectibely amplified through generations." J.Inher.Metab.Dis.15. 803-808 (1992)

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      1992 Annual Research Report
  • [Publications] K.Tominaga: "Upstream region of a genomic gene for human mitochondrial transcription factor l." Biochim.Biophys.Acta. 1131. 217-219 (1992)

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      1992 Annual Research Report
  • [Publications] H.Kamata: "Partial purification and reconstitution of inisitol 1,4,5-triphosphate receptor/Ca^2channel of bovine liver microsomes." J.Biochem.111. 546-552 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.Matsuda: "Immunohistochemical investigation of intracellular calcium pools in gerbil hippocampus following transient ischemia." Neuroscience Res.S17. S331 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.Masai: "Immaging of subunit complexes of thermophilic bacterium H^+-ATPase with scanning tunneling microscopy." UItramicroscopy. 42-44. 1194-1199 (1992)

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      1992 Annual Research Report
  • [Publications] A.Ando: "Primary structure of chitosanase produced by Bacillus circulans MH-K." J.Gen.Appl.Microbiol.38. 135-144 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Kagawa: "Mitochondrial myopathy and transcriptional control." Med.Sports Sci.(Integration of Medical and Sports Science,Karger). 37. 324-328 (1992)

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      1992 Annual Research Report
  • [Publications] S.Kawano: "Effect of proton pump inhibitor on gastric mucosa hemodynamics and tissue oxygenation in anesthetized rats." Eur.J.Pharmacol.211. 55-60 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Shimazaki: "Involvement of calmodulin and calmodulin-dependent myosin light chain kinase in blue light-dependent H^+ pumping by guard cell protoplast from Vicia faba L" Plant Physiol.99(4). 1416-1421 (1992)

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      1992 Annual Research Report
  • [Publications] K.Kusumi: "Accumulation of chloroplast tRNAGlu is temperature sensitive in virescent seedlings of rice." Plant Physiol.99(1). 142 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Yamasaki: "Inhibition of the light-induced H^+ release from uncoupled thylakoid membrones by N-ethylmaleimide." Biochem.Biophys.Res.Commun.182. 1277-1281 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] I.Iwasaki: "Two types of channels involved in the malate ion transport across the tonoplast of a Crassulacean acid metabolism plant." Plant Physiol.98. 1494-1497 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Arata: "Thermodynamics of malate transport across the tonoplast of leaf cells of CAM plants." Plant Cell Physiol.33. 873-880 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Kusumi: "Temperature-sensitive expression of chloroplast tRNAGlu in virescent mutants of rice." Research in Photosynthesis(ed.N.Murata)Kluwer,Dordrecht. (1992)

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      1992 Annual Research Report
  • [Publications] K.Kusumi: "Circadian rhythms in the regulation of phosphoenolpyruvate carbxylase from a CAM plant." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] I.Iwasaki: "Circadian rhythms in the regulation of phosphoenolpyruvate carbxylase from a CAM plant." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Fuse: "Effects of light and high temperature on the leaves of a rice mutant sensitive to solar radiation." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Kinoshita: "Dephosphorylation of the LHCII protein by light in guardcell protoplasts from Vicia faba L." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Yamasaki: "Effect of catechols on the light-induced H^+ release from uncoupled membranes." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Iba: "Chromosome walking in the region of Arabidopsis fadD locus using yeast artificial chromosomes." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Matsuyama: "Leucine dehydrogenase from Bacillus stearothermophilus:Identification of active-site lysine by modification with pyridoxal phosphate." J.Biochem.112. 258-265 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Kazuta: "Probing the pyrophosphate-binding site in potato tuber UDP-glucose pyrophosphorylase with pyridoxal diphosphate." Protein Science. 2. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Mori: "A chimeric α-glucan phosphorylase of plant type L and H isozymes.Functional role of 78-residue insertion in type L isozyme." J.Biol.Chem.268. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Takada: "Quantitative immunogold localization of Na,K-ATPase along rat nephron." Histochemistry. 98. 183-197 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Kawasaki: "Quantitative immunoelectron microscopic localization of Na,K-ATPase α-subunit in the epitherial cells of rat vestibular apparatus." Hear.Res.60. 64-72 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Okami: "UItrastructural localization of Na^+,K^+-ATPase in the exorbital lacrimal gland of rat." Invest.Ophthamol.Vis,Sci.33. 196-204 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Hayashi: "Properties of Active magnesium flux across the small intestine of guinea pig." Jpn.J.physiol.42. 561-575 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Hoshi: "Proton secretion by the enterocytes and its role in intestinal absorptive functions." Adv.Physiol.Sci.(S.K.Manchanda,W.Selvamurthy,V.M.Kumar eds.)McMillan India,Delli,. 64-70 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Nagasawa: "Purification of brush border membrane vesicles from rat renal cortex by size-exclusion chromatography." Anal.Biochem.201. 301-305 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Goda: "Carbohydrate digestive capacity in jejunum of rats subjected to simulated weightlessness." J.Nutr.Biochem.3. 172-175 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Ueda: "Human P-glycoprotein transports cortisol,aldosterone,and dexamethasone,but not progesterone." J.Biol.Chem.267. 24248-24252 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Saito: "Transcellular transport of organic cation across monolayers of kidney epithelial cell line LLC-PK_1." Am.J.Physiol.262. C59-C66 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.Hori: "Expression of renal organic cation transporter in Xenopus leavis oocytes." Blochem.J.283(3. 409 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.Hori: "Surface binding and intracellular uptake of gentamicin in the cultured kidney epithelial cell line(LLC-PK_1)." J.Pharmacol.Exp.Ther.261(3. 1200-1205 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Takano: "Transport of procainamide in a kidney epithelial cell line LLC-PK_1." Biochim.Biophys.Acta. 1108. 133-139 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Tanigawa: "Transport of digoxin by human P-glycoprotein expressed in a porcine kidney epithelial cell line (LLC-PK1)." J.Pharmacol.Exp.Ther.263(2. 840-845 (1992)

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      1992 Annual Research Report
  • [Publications] K.Ohoka: "P-Aminohippurate transport in rat renal brush-border membranes:a potential-sensitive transport system and an anion exchanger." Biol.Pharm.Bull.

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Katsura: "Characteristics of organic cation transporter in rat renal basolateral membrane." Blochim.Biophys.Acta.

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Mukuhata: "Photophosphorylation elements in Halobacteriaan A-type ATP synthase and bacterial rhodopsins." J.Bioenerg.Biomembr.24(6). 547-553 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Ihara: "Halobacterial A-ATP synthase in relation to V-ATPase." J.Exp.Biol.172. 475-485 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Mukohata: "Comparative studies on bacteriorhodopsin-type pumps:Basic physicochemical data for proton pumps." Structures and Functions of Retinal Proteins (ed.J.L.Rigaud)Colloque INSERM,John Libbey Eurotext Ltd.,Montrouge,France. 221. PP101-104 (1992)

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      1992 Annual Research Report
  • [Publications] J.Minagawa: "Identification of geme and copper ligands in subunit I of the cytochrome bo complex in Escherichia coli." J.Biol.Chem.267. 2096-2104 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Saiki: "Hemo O biosynthesis in Escherichia coli:The cyoE gene in the cytocgrome bo operon encodes a protoheme IX farnesyltransferase." Biochim.Biophys.Res.Commun.189. 1491-1497 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Tsubaki: "Structure of heme-copper binuclear center of the cytochrome bo complex in Escherichia coli:EPR and Fourier-transform infrared spectroscopic studies." Biochemistry. 32. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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