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Phylogenetic and evolutionary studies on Prunus species.

Research Project

Project/Area Number 04454053
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 園芸・造園学
Research InstitutionKobe University

Principal Investigator

YOSHIDA Masao  Kobe University Agriculture Science, Professor, 農学部, 教授 (60240313)

Co-Investigator(Kenkyū-buntansha) NOMURA Keiichi  Assistant Professor, 農学部, 助手 (60252798)
OZAKI Takeshi  Associate Professor, 農学部・附属農場, 助教授 (50031183)
中西 テツ  神戸大学, 農学部, 助教授 (80031227)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1992: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsRAPD / Phylogenetic studies / Classification / PCR / RFLP / Prunus / evolution / DNA
Research Abstract

In order to research relationships of Prunus species, We applied RAPD assay and PCR-RFLP method using three chloroplast DNA domains (psbA,rbcL-ORF106, atpB-rbcL). Matcrial plants of mumc, apricot, plum and peach were offered from Fruit Tree Research Station, and Cherries were offered from Yanmagata Horticultural Research Station. In 1992, We confirmed RAPD assay is suitable for parentage, classification of cultivars and phylogenetic studies in Prunus species using representative cultivars. In 1993, We investigated relationships among many cultivars. Mume cultivars were classified into 6 groups ; "Taiwan-mume", "Koume ", "Chuume", "Oume", "mume-apricot hybrid", "mume-plum hybrid", and it was supposed that Japanese mume is derived from China. Apricot cultivars classified into two groups ; "East Asia", "West Eurasian". Plumcultivars were largely classified into two groups ; "Japanese plum", "European plum". These mumes, apricots and plums are genetically similar and most cultivars have been evoluted with crossing. Peach cultivars are high homologous and were largely classified into two groups ; "P.persica", "P.mira". In 1995, We performed the classification of cherries by RAPD assay, and evolutionary studies of Prunus species by PCR-RFLP method. Prunus species were largely separated into "plum group" and "Cherry group" and Both group were divided in the old time. P.tomentosa, P.japonica which are classified into subgenus Cherry were classified into "Plum group". The experiment of classification of cherries was prevented by earthquake and now, we continue the last part of experiment again.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] 島田 武彦: "RAPD分析法によるウメの品種分類" 園芸学会雑誌. 62(別1). 8-9 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 武田 敏秀: "RAPD分析法のアンズ系統分類への適用" 園芸学会雑誌. 62(別2). 128-129 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田 武彦: "RAPD分析法のスモモ系統分類への適用" 園芸学会雑誌. 62(別2). 130-131 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田 武彦: "モモ系統分類におけるRAPD分析法の有効性について" 育種学会雑誌. 43(別2). 213 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada,T.: "Classification and parent determination by RAPD in mume." Techniques on Gene Diagnosis and Breeding in Fruit Tree. 77-80 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada,T.: "Phylogenetic studies on mume cultivars(Prunus mume Sied.et Zucc)in Japan.21GC06:Internation Symposium on Cultivar Improvement of Horticultural" Abst.259 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 孫 賽初: "RAPD分析法によるモモ野生種の分類" 園芸学会雑誌. 63(別1). 4-5 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 武田 敏秀: "RAPD分析法によるアンズの品種分類" 園芸学会雑誌. 63(別1). 80-81 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田 武彦: "RAPD分析法によるスモモ亜属及びモモ亜属の系統分類" 園芸学会雑誌. 63(別1). 82-83 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada,T.: "Phylogenetic studies on plum-mume and plum-apricot hybrids by RAPD analysis." 24th Internationnal Horticultural Congress.Abst.223 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takeda,T.: "Phylogenetic studies on mume-apricot hybrids by RAPD analysis." 24th International Horticultural Congress.Abst.223 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田 武彦: "オルガネラDNA領域を用いたPCR-RELPによるサクラ属植物の系統分類" 育種学会雑誌. 44(別2). 107 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada,T.: "Classification of mume(Prunus mume Sied.et Zucc)by RAPD assay." J.Japan Soc.Hort.Sci.63(3). 543-551 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田 武彦: "PCRマーカーによるスモモの系統分類" 園芸学会雑誌. 64(別1). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshida,M.: "Phylogenetic studies on Prunus species by DNA finger prints" 92nd Ann.Meet.of Amer.Soc.Hort.Sci.and 40th Congr of Canad.Soc.Hort Sci. Abst.(1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada T., K.Hosaka, T.Nakanishi, T.Ozaki, M.Yoshida, J.Yamamoto and K.Fujimoto: "Classfication and parent determination by RAPD in mume" J.Japan.Soc.Hort.Sci. 61 (Suppl.l) : (In Japanese). 140-141 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, T., T.Haji, M.Yamaguchi, T.Nakanishi, T.Ozaki, M.Yoshida.: "Classification by RAPD analysis in mume (Prunus mume Sieb.et Zucc.)" J.Japan.Soc.Hort.Sci. 62 (Suppl.l) : (In Japanese). 8-9 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takeda.T., T.Shimada, T.Haji, M.yamaguchi, K.Nomura, M.Yoshida.: "RAPD analysis applied to phylogenetic studies in apricots." J.Japan.Soc.Hort.Sci. 62 (Suppl.2) : (In Japanese). 128-129 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada.T., T.Haji, M.Yamaguchi, K.Nomura, M.Yoshida.: "RAPD analysis applied to phylogenetic studies in plums." J.Japan.Soc.Hort.Sci. 62 (Suppl.2) : (In Japanese). 130-131 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, T., T.Haji, M.Yamaguchi, M.Yoshida.: "RAPD analysis applied to phylogenetic studies in peaches." Japan.J.Breed. 43 (Suppl.2) : (In Japanese). 213 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, T., Haji, T.and Hosaka, K: "Classification and parent determination by RAPD in mume" Technique on Gene Diagnosis and Breeding in Fruit Trees (ed, T,Hayashi et al.) 77-80, Copyright 1993 FTRS/Japan.1993.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, T., Hosaka, K., Nakanishi, T., Yoshida, M., Haji, T.and Yamaguchi, M: "Phylogenetic studies on mume cultivars (Prunus mume Sieb.et Zucc.) in Japan" International Symposium on Cultivar Improvement of Horticultural Crops, Held Jointly with the Peach Congress.Abstracts.259. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sun, S,M.Yoshida, T.Shimada, T.Takeda, M.Yamaguchi, T.Haji.: "Classification by RAPD analysis in wild peach species." J.Japan.Soc.Hort.Sci.63 (Suppl.l) : (In Japanese). 4-5 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Takeda, T.Shimada, Q.Cao, K.Nomura, M.Yoshida, T.Haji, M.Yamaguchi: "Classification by RAPD analysis in apricot varieties." J.Japan.Soc.Hort.Sci.63 (Suppl.l) : (In Japanese). 80-81 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, T., T.Haji, M.Yamaguchi, M.Yamaguchi, K.NAOMURA,T.Ozaki, M.Yoshida.: "Phylogenetic studies in prunphora.Amygdalus by RAPD analysis." J.Japan.Soc.Hort.Sci.63 (Suppl.l) : (In Japanese). 82-83 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, T., M.Toyofuku, T.Haji, M.Yamaguchi, and M.Yoshida.: "Phylogenetic studies on plum-mume and plum-apricot hybrids by RAPD analysis." 24th International Horticultural Congress.Abstracts.223.(1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takeda T., T.Shimada, T.Haji, M.Yamaguchi, K.Nomura, and M.Yoshida.: "Phylogenetic studies on mume-apricot hybrids by RAPD analysis." 24th International Horticultural Congress.Abstracts.223. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada T., T.Haji, M.Yamaguchi, T.Takeda, K.Nomura and M.Yoshida.: "Classification of mume (Prunus mume Sieb.et Zncc.) by RAPD assay" J.Japan.Soc.Hort.Sci.vol.63, (3). 543-551 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, T., T.Haji, M.Yamaguchi, M.Yoshida.: "Phylogeny of genus Prunus by PCR-RFLP method using a special region of organera DNA." Japan.J.Breed.44 (Suppl.2) : (In Japanese). 107 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, T., M.Toyofuku, T.Shiratori, T.Haji, M.Yamaguchi, M.Yoshida.: "Phylogenetic studies in plum by PCR markers." J.Japan.Soc.Hort.Sci.64 (Suppl.1) : (In Japanese). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshida, M., Shimada, T.and Yamaguchi, M.: "Phylogenetic studies on Prunus species by DNA fingerprints." 92nd Annual Meeting of Amer.Soc.Hort.Sci.and 40th Congress of Canada.Soc.Hort.Sci.Abstr.? ? ? .(1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada,T.: "Classification of mume(Prunus mume Sieb.et Zucc)by RAPD assay." J.Japan Soc.Hort.Sci.63(3). 543-551 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 武田 敏秀: "RAPD分析法によるアンズの品種分類" 園芸学会雑誌. 63(別1). 80-81 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 島田 武彦: "RAPD分析法によるスモモ亜属及びモモ亜属の系統分類" 園芸学会雑誌. 63(別1). 82-83 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 孫 賽初: "RAPD分析法によるモモ野性種の分類" 園芸学会雑誌. 63(別1). 4-5 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimada,T.: "Phylogenetic studies on plum-mume and plum-apricot hybrids by RAPD analysis." 24th International Horticultural Congress.(Abst). 223- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takeda,T.: "Phylogenetic studies on mume-apricot hybrids by RAPD analysis." 24th International Horticultural Congress.(Abst). 223- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 島田 武彦: "オルガネラDNA領域を用いたPCR-RFLPによるサクラ属植物の系統分類" 育種学会雑誌. 44(別2). 107 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 島田 武彦: "PCRマーカーによるスモモの系統分類" 園芸学会雑誌. 64(別1). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshida,M.: "Phylogenetic studies on Ptunus species by DNA finger prints" 92th Ann.Meet.of Amer.Soc.Hort.Sci.and 40th Congr.(Abst.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimada,T.et al.: "Classification and parent determination by RAPD in mume." Techniques on Gene Diagnosis and Breeding in Fruits Trees. 77-80 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 島田 武彦 他: "モモ系統分類におけるRAPD分析法の有効性について" 育種学雑誌. 43(別2). 213 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 島田 武彦 他: "RAPD分析法によるウメの品種分類" 園芸学会雑誌. 別1. 8-9 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 島田 武彦 他: "RAPD分析法によるスモモ系統分類への適用" 園芸学会雑誌. 別2. 130-131 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimada,T.et al.: "Phylogenetic studies on mume cultivars (Prunus mume Sieb.et Zucc.) in Japan" International Symposium on Improvement of Horticultural Crops,Held Jointly with the Peach Congress. 259 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 島田 武彦・上師岳・山口 正巳・中西 テツ・尾崎 武・吉田 雅夫: "RAPD分析法によるウメの品種分類" 園芸学会雑誌. 62(別1). (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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