|Budget Amount *help
¥6,700,000 (Direct Cost : ¥6,700,000)
Fiscal Year 1993 : ¥1,300,000 (Direct Cost : ¥1,300,000)
Fiscal Year 1992 : ¥5,400,000 (Direct Cost : ¥5,400,000)
At first, the nucleotide sequences of the L gene of Paramyxovirus, simian viruses 5 and 41 were determined. Therefore, amino acid sequences of components of replicative complex, NP, P, and L proteins were also determined. Full-length cDNA clones for NP,P, and L protein could be obtained. Next, artificial mini-genomes, Leader sequence-R1-reporter gene-R2-Trailer sequence, were constructed. The mini-genomes were transfected into human parainfluenza type 2 virus or simian virus 41-infected cells. In this system, replication, transcription and RNA editing were occurred. However, the efficiency was not so high. Subsequently, the mini-genomes, cDNA for NP, P, and L proteins were transfected into the cells infected recombinant vaccinia virus containing T7 polymerase. In this new system, high efficiency of transcription, replication and RNA editing were observed. In another experiment, full-length cDNA clone for human parainfluenza virus type 2 was nearly constructed.