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Mechanism of the Release of Acetylcholine from Presynaptic Nerve Terminals Isolated from Electric Organ of Japanese Electric Ray

Research Project

Project/Area Number 04454526
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionThe University of Tokyo (1993)
Kyushu University (1992)

Principal Investigator

KIRINO Yutaka  The University of Tokyo, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (10012668)

Co-Investigator(Kenkyū-buntansha) 平嶋 尚英  九州大学, 薬学部, 助手 (10192296)
安西 和紀  九州大学, 薬学部, 助手 (70128643)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsElectric organ / Presynaptic terminal / Acetylcholine / Exocytosis / Mast cells / Calcium channel / Degranulation / Asymmetric distribution of phospholipids / シナプトソーム / シビレエイ / 電気器官 / 神経シナプス / Caチャネル / 神経伝達物質の遊離
Research Abstract

1. Action and binding of various Ca channel blockers were examined on the calcium channels triggering the release of acetylcholine (ACh) in the synaptosomes prepared form the electric organ of Japanese electric ray, Narke japonica. The data obtained suggests that the Ca channels subserving the ACh release are mainly of N- and P-types and the L-type also has a small contribution. 2. A monoclonal antibody, MCC-1, that recognizes the alpha _2 delta subunit of rabbit skeletal muscle, inhibited partially the ACh release. 3. Immunoaffinity chromatography using MCC-1 was employed to solubilized plasma membrane of the synaptosomes in order to purify proteins with affinity to MCC-1. The SDS-PAGE analysis and Western blotting of the purified fraction identified a 170 kDa polypeptide as an MCC-1 binding protein. 4. The purified fraction also contained syntaxin, which is known to be an essential protein for the membrane fusion. 5. Incorporation of the synaptosomes into a planar lipid membrane revealed the presence of a K channel but failed to show the presence of Ca channels. 6. A novel radioactive probes were developed to measure phospholipid translocation across the biomembranes. With this probe, it was found that synaptosomal plasma membrane contains a phoshatidylserine-specific translocase. On the other hand, no specific translocase was found in synaptic vesicles. 7. A simultaneous measurement of the degranulation and intracellular Ca change in rat peritoneal mast cells implicated that intracellular Ca rise is a prerequisite for degranulation. 8. Membranes of the secretory granules of mast cells contain a cation-channel which may be responsible for the formation of so-called fusion pore.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (68 results)

All Other

All Publications (68 results)

  • [Publications] Naohide Hirashima,Yutaka Kirino: "A calcium-dependent cation channel in mast cell granule membranes reconstituted into a lipid bilayer" Biochem.Biophys.Res.Commun.185. 793-799 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuyuki Yoshioka,Kazunori Anzai,Yutaka Kirino: "Measurement of phospholipid translocation across plasma membranes with novel radioactive phospholipid probes" J.Pharmacobio-Dyn.15. s-76 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Iwata,S.Lee,O.Oishi,H.Aoyagi,M.Ohno,K.Shinozaki,K.Anzai,Y.Kirino: "Ion-channel formation by various amphipathic peptides with the chain length to span phospholipid bilayers-Mainly using 3_<10>-helical peptides-" Peptide Chamistry. 1992. 175-178 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Tokumaru,Kazunori Anzai,Teruo Abe,Yutaka Kirino: "Purification of the cardiac 1,4-dihydropyridine receptor using immunoaffinity chromatography with a monoclonal antibody against the δ_2α subunit of the skeletal muscle di-hydropyridine receptor" Eur.J.Pharmacol.227. 363-370 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.O'Hori,C.-Y.Wang,H.Tokumaru,L.-C.Chen,K.Hatanaka,N.Hirashima,Y.Kirino: "Action and binding of omega-conotoxin on the putative calcium channel of synaptosomal plasma membrane from electric organ of Japanese ray,Narke japonica" Neuroscience. 54. 1043-1050 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Tanaka,K.Matsumoto,S.Lee,G.Sugihara,H.Aoyagi,K.Kazunori,Y.Kirino: "Interaction of acidic amphiphilic α-helical peptides with lipid bilayers and their ion channel formation" Peptide Chemistry. 1992. 681-684 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Anzai,Y.Yoshioka,Y.Kirino: "Novel radioactive phospholipid probes as a tool for measurement of phospholipid translocation across biomembranes" Biochim.Biophys.Acta. 1151. 69-75 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Lee,T.Iwata,H.Oyagi,H.Aoyagi,M.Ohno,K.Anzai,Y.Kirino,G.Sugihara: "Effect of Salts on conformational change of basic amphipathic peptides from β-structure to α-helix in the presence of phospholipid liposomes and their channel forming activity" Biochim.Biophys.Acta. 1151. 76-82 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Toda,N.Hirashima,K.Kuba,S.Kawahara,H.Shimizu,Y.Kirino: "Spatio-temporal changes in intracellular calcium concentrations in giant neuronal cells from a terrestrial slug,Incilaria bilineata,upon stimulation by depolarization and caffeine" Bioimages. 1. 95-103 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Anzai,M.Masumi,K.Kawasaki,Y.Kirino: "Effects of lipid charge in planar bilayer membranes on the fusion of liposomes containing nystatin-ergosterol channels to the bilayers" J.Biochem.114. 487-491 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Tokumaru,C.-Y.Wang,N.Hirashima,T.O'Hori,H.Yamamoto,Y.Kirino: "Action and binding of calcium channel blockers on the putative calcium channel of synaptosomal plasma membrane from the electric organ of Narke japonica" Annals New York Acad.Sci.707. 410-412 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Iwata,S.Lee,O.Oishi,H.Aoyagi,M.Ohno,K.Anzai,Y.Kirino,G.Sugihara: "Design and synthesis of amphipathic 3_<10>-helical peptides and their interactions with phospholipid bilayers and ion channel formation" J.Biol.Chem.(in press). (199)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Lee,T.Tanaka,K.Anzai,Y.Kirino,H.Aoyagi,G.Sugihara: "Two mode ion channes induced by interaction of acidic amphipathic α-helical peptides with lipid bilayers" Biochim.Biophys.Acta. (in press). (199)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Shinozaki,K.Anzai,Y.Kirino,S.Lee,H.Aoyagi: "Ion channel activity of a synthetic peptide with a primary structure corresponding to the presumed pore-forming region of the voltage-dependent potassium channel" Biochem.Biophys.Res.Commun.(in press). (199)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Anzai,C.Takano,K.Tanaka,Y.Kirino: "Asymmetric lipid charge changes the substate conductance of the potassium channel from sarcoplasmic reticulum" Biochem.Biophys.Res.Commun.(in press). (199)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 安西和紀、桐野豊: "骨格筋小胞体Ca^<2+>ポンプの起電性-新しい実験法による研究の新展開" 実験医学. 10. 80-85 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 高木博、桐野豊、吉岡亨: "ラット小脳プルキンエ細胞のCa依存性K電流のアラキドン酸による調節" 神経研究の進歩. 36. 926-932 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 安西和紀、桐野豊: "イオン輸送システム" 蛋白質核酸酵素. 38. 1187-1192 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Kinosita,M.Hibino,H.Itoh,M.Shigemori,K.Hirano,Y.Kirino: "Guide to Electroporation and Electrofusion" Academic Press, 581 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 桐野豊: "最新医学からのアプローチ:イオンチャネル1" メジカルビュー, 230 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 桐野豊: "レセプター:基礎と臨床" 朝倉書店, 944 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naohide Hirashima and Yutaka Kirino: ""A Calcium-dependent Cation Channel in Mast Cell Granule Membranes Reconstituted into a Lipid Bilayr"" Biochem.Biophys.Res.Commun.185. 793-799 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuyuki Yoshioka, Kazunori Anzai and Yutaka Kirino: ""Measurement of Phospholipid Translocation across Plasma Membranes with Novel Radioactive Phospholipid Probes"" J.Pharmacobio-Dyn.15. s-76 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshihide Iwata, Sannamu Lee, Osamu Oishi, Haruhiko Aoyagi, Motonori Ohno, Kouki Shinozaki, Kazunori Anzai, and Yutaka Kirino: ""Ion-Channel Formation by Various Amphipathic Peptides with the Chain Length to Span Phospholipid Bilayrs-Mainly Using 3_<10>-Helical Peptides-"" Peptide Chemistry 1991, (A.Suzuki, Ed.) Protein Research Foundation, Osaka. 175-178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Tokumaru, Kazunori Anzai, Teruo Abe and Yutaka Kirino: ""Purification of the Cardiac 1,4-Dihydropyridine Receptor Using Immunoaffinity Chromatography with a Monoclonal Antibody against the alpha _2delta Subunit of the Skeletal Muscle Dihydropyridine Receptor"" Eur.J.Pharmacol. 227. 363-370 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomohiro O'Hori, Chen-Yueh Wang, Hiroshi Tokumaru, Li-Chy Chen, Ken'ichi Hatanaka, Naohide Hirashima and Yutaka Kirino: ""Action and Binding of Omega-Conotoxin on the Putative Calcium Channel of Synaptosomal Plasma Membrane from Electric Organ of Japanese Electric Ray, Narke japonica"" Neuroscience. 54. 1043-1050 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taichi Tanaka, Kenji Matsumoto, Sannamu Lee, Gohsuke Sugihara, Haruhiko Aoyagi, Kazunori Anzai and Yutaka Kirino: ""Interaction of Acidic Amphiphilic alpha -Helical Peptides with Lipid Bilayrs and Their Ion Channel Formation"" Peptide Chemistry 1992, Protein Research Foundation. 681-684 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazunori Anzai, Yasuyuki Yoshioka and Yutaka Kirino: ""Novel Radioactive Phospholipid Probes as a Tool for Measurement of Phospholipid Translocation across Biomembranes"" Biochim.Biophys.Acta.1151. 69-75 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sannamu Lee, Toshihide Iwata, Hatsuyo Oyagi, Haruhiko Aoyagi, Motonori Ohno, Kazunori Anzai, Yutaka Kirino and Gohsuke Sugihara: ""Effect of Salts on Conformational Change of Basic Amphipathic Peptides from beta -Structure to alpha -Helix in the Presence of Phospholipid Liposomes and Their Channel-forming Activity"" Biochim.Biophys.Acta.1151. 76-82 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shoichi Toda, Naohide Hirashima, Kenji Kuba, Shigenori Kawahara, Hiroshi Shimizu and Yutaka Kirino: ""Spatiotemporal Changes in Intracellular Calcium Concentrations in Giant Neuronal Cells form a Terrestrial Slug, Incilaria bilineata, upon Stimulation by Depolarization and by Caffeine"" Bioimages. 1. 95-103 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazunori Anzai, Masako Masumi, Koh Kawasaki and Yutaka Kirino: ""Effects of Lipid Charge in Planar Bilayr Membranes on the Fusion of Liposomes Containing Nystatin-Ergosterol Channels to the Bilayrs"" J.Biochem.114. 487-491 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Tokumaru, Chen-Yueh Wang, Naohide Hirashima, Tomohiro O'Hori, Haruhiko Yamamoto and Yutaka Kirino: ""Action and Binding of Calcium Channel Blockers on the Putative Calcium Channel of Synaptosomal Plasma Membrane from the Electric Organ of Narke japonica"" Annuals New York Acad.Sci.707. 410-412 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshihide Iwata, Sannamu Lee, Osamu Oishi, Haruhiko Aoyagi, Motonori Ohno, Kazunori Anzai, Yutaka Kirino and Gohsuke Sugihara: ""Design and Synthesis of Amphipathic 3_<10>Helical Peptides and Their Interactions with Phospholipid Bilayrs and Ion Channel Formation"" J.Biol.Chem.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sannamu Lee, Taichi Tanaka, Kazunori Anzai, Yutaka Kirino, Haruhiko Aoyagi and Gohsuke Sugihara: ""Two Mode Ion Channels Induced by Interaction of Acidic Amphipathic alpha -Helical Peptides with Lipid Bilayrs"" Biochim.Biophys.Acta.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohki Shinozaki, Kazunori Anzai, Yutaka Kirino, Sannamu Lee and Haruhiko Aoyagi: ""Ion Channel Activity of a Synthetic Peptide with a Primary Structure Corresponding to the presumed Pore-Forming Region of the Voltage-Dependent Potassium Channel"" Biochem.Biophys.Res.Commun.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazunori Anzai, Chika Takano, Keiko Tanaka and Yutaka Kirino: ""Asymmetrical Lipid Charge Changes the Substate conductance of the Potassium Channel from Sarcoplasmic Reticulum"" Biochem.Biophys.Res.Commun.(submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazunori Anzai and Yutaka Kirino: ""Electrogenicity of the Ca^<2+> Pump of Skeletal Muscle Sarcoplasmic Retriculum" [in Japanese]" Jikken-Igaku. 10. 80-85 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Takagi, Yutaka Kirino and Tohru Yoshioka: ""Regulation by Arachidonic Acid of Ca-dependent K Current in Purkinje Cells of Rat Cerebellum"" Shinkei-Kenkyuno-Shimpo. 36. 926-932 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazunori Anzai and Yutaka Kirino: ""Ion Transporting System" [in Japanese]" Tampakushitsu-Kakusan-Kohso. 38. 1187-1192 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazuhiko Kinosita, Jr., Masahiro Hibino, Hiroyasu Itoh, Masaya Shigemori, Ken-ichi Hirano, Yutaka Kirino and Tsuyoshi Hayakawa: "Events of Membrane Electroporation Visualized on a Time Scale from Microsecond to Seconds." In "Guide to Electroporation and Electrofusion" (D.C.Chang, B.M.Chassy, J.A.Saunders and E.E.Sowers, Eds.). Academic Press, New York, 29-46 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yutaka Kirino: "General Properties and Classification of Ion Channels in "Ion Channels (H.Higashida, ed.)" [in Japanese]. Medical View, 24-33 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yutaka Kirino: "Functional Reconstitution of Ion Channels" in "Receptors (H.Imura, T.Oka, T.Haga and E.Kishimoto, Eds.)" [in Japanese]. Asakura Publishing Co., 272-278 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T O'Hori,C-Y Wang,H Tokumaru,…,Y Kirino: "Action and binding of omega-conotoxin on the putative Ca channel of synaptosomal membrane from electric organ of Japanese ray" Neuroscience. 54. 1043-1050 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T Tanaka,K Matsumoto,S Lee,…,Y Kirino: "Interaction of acidic amphiphilic α-helical peptide with lipid bilayers and their ion channel formation" Peptide Chemistry. 1992. 681-684 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K Anzai,Y Yoshioka,Y Kirino: "Novel radioactive phospholipid probes as a tool for measurement of phospholipid translocation across biomembranes" Biochim.Biophys.Acta. 1151. 69-75 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S Lee,T Iwata,H Oyagi,…,Y Kirino,G Sugihara: "Effect of salts on conformational change of basic amphipathic peptides and their channel-forming activity" Biochim.Biophys.Acta. 1151. 76-82 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S Toda,N Hirashima,K Kuba,Y Kirino: "Spatio-temporal changes in intracellular Ca concentrations in giant neuronal cells from a terrestrial slug upon stimulation" Bioimages. 1. 95-103 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K Anzai,M Masumi,K Kawasaki,Y Kirino: "Effects of lipid charge in planar bilayer membranes on the fusion of liposomes containg nystatin-ergosterol channels with the…" J.Biochem.114. 487-491 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H Tokumaru,C-Y Wang,N.Hirashima,…,Y Kirino: "Action and binding of Ca channel blockers on the putative Ca channel of synptosomal plasma membrane from the electric…" Annals New York Acad.Sci.707. 410-412 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T Iwata,S Lee,O Oishi,…,Y Kirino,G Sugihara: "Design and synthesis of amphipathic 3_<10> helical peptides and their interactions with phospholipid bilayers and ion channel formation" J.Biol.Chem.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] S Lee,T Tanaka,K Anzai,Y Kirino,…,G Sugihara: "Two mode ion channels induced by interaction of acidic amphipathic α-helical peptides with lipid bilayers" Biochim.Biophys.Acta. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K Shinozaki,K Anzai,Y Kirino,S Lee,H Aoyagi: "Ion channel activity of a synthetic peptide with a primary structure corresponding to the presumed pore-forming region of the…" Biochem.Biophys.Res.Commun.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K Anzai,C Takano,K Tanaka,Y Kirino: "Asymmetric lipid charge changes the substate conductance of the potassium channel from sarcoplasmic reticulum" Biochem.Biophys.Res.Commun.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 安西和紀、桐野豊: "イオン輸送システム" 蛋白質核酸酵素・特集号「生体 超分子システム」. 38. 1187-1192 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 桐野豊、他: "イオンチャネル1" メジカルビュー社, 230 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 桐野豊、他: "レセプター" 朝倉書店, 944 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Naohide Hirashima,Yutaka Kirino: "A calcium-dependent cation channel in mast cell granule membranes reconstituted into a liped bilayer" Biochem.Biophys.Res.Commun.185. 793-799 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasuyuki Yoshioka,Kazunori Anzai,Yutaka Kirino: "Measurement of phospholipid translocation across plasma membranes with novel radioactive phospholipid probes" J.Pharmacobio-Dyn.15. S-76 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Iwata.S.Lee,O.Oishi,H.Aoyagi,M.Ohno,K.Shinozaki,K.Anzai,Y.Kirino: "Ion-channel formation by various amphipathic peptides with the chain length to phospholipid bilayers-Mainly using 3_<10>-helical peptides-" Peptide Chamistry. 1992. 175-178 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroshi Tokumaru,Kazunori Anzai,Teruo Abe, Yutaka Kirino: "Purification of the cardiac 1,4-dihydropyridine receptor using immunoaffinity chromatography with a monoclonal antibody against the δ_2α subunit of the skeletal muscle di-hydropyridine receptor" Eur.J.Pharmacol.227. 363-370 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.O'Hori,C.-Y.Wang,H.Tokumaru,L.-C.Chen,K.Hatanaka,N.Hirashima,Y.Kirino: "Action and binding of ω-conotoxin on the putative calcium channel of synaptosomal plasma membrane from electric organ of japanese ray,Narke japonica" Neuroscience. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Tokumaru,C.-Y.Wang,N.Hirashima,T.O'Hori,H.Yamamoto,Y.Kirino: "Action and binding of calcium channel blockers on the putative calcium channel of synaptosomal plasma membrane from the electric organ of Narke japonica" Ann.New York Acad.Sci.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 安西 和紀、桐野 豊: "骨格筋小胞体Ca^<2+>ポンプの起電性-新しい実験法による研究の新展開" 実験医学. 10. 80-85 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 高木 博、桐野 豊、吉岡 亨: "ラット小脳プルキンエ細胞のCa依存性K電流のアラキドン酸による調節" 神経研究の進歩. 36. 926-932 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 安西 和紀、桐野 豊: "イオン輸送システム" 蛋白質核酸酵素. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Kinosita,M.Hibino,H.Itoh,M.Shigemori,K.Hirano,Y.Kirino: "Guide to Electroporation and Electrofusion" Academic Press, 581 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 桐野 豊: "最新医学からのアプローチ:イオンチャネル" メジカルビュー,

    • Related Report
      1992 Annual Research Report
  • [Publications] 桐野 豊: "レセプター:基礎と臨床" 朝倉書店,

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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