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Radiation (Low Dose) -induced Apoptosis and It's Susceptibility Genes

Research Project

Project/Area Number 04454606
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 放射線5生物学
Research InstitutionOsaka University

Principal Investigator

NOMURA Taisei  Osaka Univ., Fac.Med., Professor, 医学部, 教授 (90089871)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Hiroo  Osaka Univ., Fac.Med., Ass.Professor, 医学部, 助手 (20237275)
TODO Takeshi  Osaka Univ., Fac.Med., Ass.Professor, 医学部, 講師 (90163948)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsApoptosis / Interphase Cell Death / Mouse Thymus / Low Dose Radiation / Dominant Gene (Apo-1) / Recessive Genes / Programd Cell Death / RI Strain / 放射線 / 酵素反応の介在
Research Abstract

1) Dominant and recessive genes controling susceptibility to radiation induced apoptosis in the mouse thymus : We established sensitive method to detect apoptosis by staining dead or dying cells with erythrosines B in the frozen section of the organs (thymus, spleen, intestine, etc.). Single dominant gene showing Menderian inheritance was found by the genetic cross of radiation-induced apoptossis sensitive strain N4 and resistant C3H/HeJ.The examinations using linkage stocks and microsatellite markers revealed that this gene was linked to the chromosome 4. Another 2 recessive genes controling susceptibility were found by the use of recombinant inbred strain (BHX) of senstive strain C57BL/6J and resistant C3H/HeJ.
2) Massive suicide deaths of thymic Iymphocytes : By the specific stainig method of the frozen sections, we found that thymic cells died massively but not singly at early stages (2-8 hours) after irradiattion. Dead cells were eliminated after then and it looks like single cell death 24 hours after irradation. Apoptosis of Iymphocytes in the cultured thymus was inhibited by low temparature and cyclophosphamide (inhibitor of protein synthesis) .
3) Realation to Lymphocytic leukemia : Mouse strains showing high susceptibility to radiation-induced apoptosis are also susceptible to radiation-induced leukmia, but the analysis by RI strain showed that genes concerned to these disorders are different.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Nomura,T.: "Programmed cell death in whole body and organ system by low dose radiation." J.Radiat.Res.33. 109-123 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takatera,H.: "Sifferential expression of a human sperm-specific isozyme in seminoma cells transplanted into scid-nustr mice." Jpn.J.Cancer Res.84. 215-218 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Todo,T.: "A new photoreactivating enzyme that specifically repairs ultraviolet light-induced(6-4) phtoproducts." Nature. 361. 371-374 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nomura,T.: "Leukaemia in children whose parents have been exposed to radiation." Br.Med.J.306. 1412 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakajima,H: "Enhancement of tumor growth under short light/dark cycle in the mouse lung." Cancer Letters. 78. 127-131 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizutani,T.: "Identification of estrogen receptorin human adipose tissue and adipocytes." J.Clin.Endocrinol.Metab.78. 950-954 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nomura,T.: "Low Dose Irradia ion and Biological Defense Mechanism" Elsevier,Amsterdam, 7 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nomura,T.: "Maile-mediated Developmental Toxicity" Plenum Press,New York, 11 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nomura, T.: "Programd cell death in whole body and organ system by low dose radiation." J.Radiat.Res.33. 109-123 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takatera, H.: "Differential expression of human sperm specific isozyme in seminoma cells transplanted into scid - nu^<str> mice." Jpn.J.Cancer Res.84. 215-218 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Todo, T.: "A new photoreactivating enzyme that specifically repairs ultraviolet high-induced (6-4) photoproducts." Nature. 361. 371-374 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nomura, T.: "Leukaemia in children whose parents have been exposed to radiation." Br.Med.J.306. 1412 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakajima, H.: "Enhancement of tumor growth under short ligh/dark cycle in the mouse lung." Cancer Letters. 78. 127-131 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizutani.T.: "Indentification of estrogen receptor in human adipose tissue and adipocytes." J.Clin.Endocrinol.Metab.78. 950-954 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nomura, T.: Loe Dose Irradiation and Biological Defense Mechanism.Elsevier, Amsterdam, 7 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nomura, T.: Male-mediated Developmental Toxicity. Plenum Press, New York, 11 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizutani,T.: "Identification of estrogen receptorin human adipose tissue and adipocytes." J.Clin.Endocrinol.Metab.78. 950-954 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohsugi,T.: "Engraftment of HTLV-1-ransformed human T-cell line into SCID mice with NK cell function." J.Vet.Med.Sci.56. 601-609 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Enomoto,T.: "Analysis of clonality by amplification of short tandem repeats." Diag.Mol.Pathol.3. 292-297 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Todo,T.: "High-level expression of the photorepair gene in Drosophila ovary and its evolutionary implications." Mutat.Res.315. 213-228 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato,T.: "Cloning of a marsupial DNA photolyase gene and the lack of related nucleotide sequences in placental mammals." Nucl.Acids Res.,. 22. 4119-4124 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakajima,H.: "Enhancement of tumor growth under short light/dark cycle in the mouse lung." Cancer Letters. 78. 127-131 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nomura,T.: "Male-mediated Developmental Toxicity" Plenum Press,New York, 11 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Todo,T.: "A new photoreactivating enzyme that specifically repairs ultraviolet light-induced(6-4)phtoproducts." Nature. 361. 371-374 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takatera,H.: "Differential expression of a human sperm-specific isozyme in seminoma cells transplanted into scid-nu^<str> mice." Jpn.J.Cancer Res.84. 215-218 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nomura,T.: "Leukaemia in children whose parents have been exposed to radiation." Br.Med.J.306. 1412 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Gotoh,H.: "Growth inhibition of transplanted murine breast cancer by nicotinamide in C3H/HeJ mice." Cancer Research,Therapy and Control. 3. 121-126 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohsugi,T.: "Isolation of acinetobacter calcoaceticus from the gastrointestinal tracts of SCID mice." Laboratory Animals. 27. 226-228 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakajima,H.: "Enhancement of tumour growth under short light/dark cycle in the mouse lung." Cancer Letters. (印刷中). (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nomura,T.: "Low Dose Radiation and Living State" Narosa Publ., 17 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nomura,T.: "Male-mediated Developmental Toxicity" Plenum Press(印刷中), (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nomura,T.: "Programmed cell death in whole body and organ system by low dose radiation." Journal of Radiation Research. 33. 109-123 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ohuchi,Y.: "Oncogenic activation of murine mos protein kinase by DNA rearangement of it's N-terminal coding region." Oncogene. 7. 331-338 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Todo,T.: "A new photoreactivating enzyme that specifically repairs ultraviolet light-induced (6-4) photoproducts." Nature. 361. 371-374 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sasai,H.: "Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours:detection of transforming genes in X-ray-related mouse tumours." British Journal of Cancer. 67. 262-267 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ohmori,H.: "Comparison of Ki-ras gene mutation among simultaneously occurring multiple urethane-induced lung tumorg in individual mice." Carcinogenesis. 13. 851-855 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tanaka,T.: "The scid mutation in mice causes defects in the repair system for both double-strand DNA breaks and DNA-cross links." Mutation Research. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nomura,T.: "Low Dose Irradiation and Biological Defense Mechanism" Elsevier, 510 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nomura,T.: "Male-mediated Developmental Toxicity" Plenum Press, (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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