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Development of microdialysis probe for the kidney

Research Project

Project/Area Number 04557010
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKagawa Medical School

Principal Investigator

ABE Youichi  Kagawa Medical School, Department of Pharmacology, Professor, 医学部, 教授 (10047227)

Co-Investigator(Kenkyū-buntansha) AKI Yasuharu  Kagawa Medical School, Department of Pharmacology, Assistant, 医学部, 助手 (50231816)
TAMAKI Toshiaki  Kagawa Medical School, Department of Pharmacology, Assistant Professor, 医学部, 助教授 (80179879)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1992: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsMicro-dialysis / Fiber-type probe / Adenosine / Inosine / Hypoxanthin / Kidney / Ischemia / Recirculation
Research Abstract

The "bio-sampling" technique called microdialysis was originally developed by Ungerstedt and colleagues. The principle of this method is to extract metabolizes from the extracellular fluid(ECF) without removal of fluid from the tissue. The basic device is a thin (0.65 mm diameter) double lumen probe with a seim-permeable polycarbonate membrane at the tip. The probe is perfused with an isotonic saline. Small molecules cross the probe membrane by simple difusion along a concetration gradient between the perfusate and the ECF.The dialysate microsample content of various metabolizes is measured by sensitive analytical techniques including HPLC.Thus, microdialysis can be used for temporal studies of regional changes in the ECF content of various metabolizes. The method was originally designed for studies in brain tissue. However, the diameter of the microdialysis probe is 0.65 mm. This size is relatively large for the application to the kidney which is perfused at high blood flow rate. Moreover, since the kidney moves in synchronicity with the respiration, the fixation of probe is difficult. In addition, to observe the rapid responses to various stimuli high dialysis efficiency will be required for probe. Thus, in order to satisfy the above points, we have tried to develop a new microdialysis probe for the kidney. The diameter of the new probe is only 0.2 mm and the dialysis efficiency is 5 - 10 times greater than the commercial probe. Using this newly developed probe we have examined the intrarenal adenosine metabolism during ischemia and recirculation. Adenosine concentration(conc.) at basal condition was around 100 nM.Ischemia increased the adenosine conc.about 2 times. However, after the inhibition of adenosine deaminase with EHNA the adenosine conc.was increased more than 50 - 100 times during ischemia. On the other hand, adenosine conc.after the inhibition of adenosine kinase with iodotubercidine increased only 3 - 4 times. Thus, the adenosine released from the ce

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Toshiaki Tamaki: "Control of renal circulation-with special reference on nitric oxide" Asia Pacific Journal of Pharamacology. in press. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuharu Aki: "Nitric oxide may participate in V_2vasopressin-receptor-mediated-renal vasodilation." J.Cardiovasc.Pharmacol.23. 331-336 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiaki Tamaki: "Effects of EXP3174,a non-peptide angiotensin II receptor antagonist,on renal hemodynamics and renal funkition in doks" Eur.J.Pharmacol.236. 15-21 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiaki Tamaki: "Effects of N^G-nitro-arginie on ieolated rabbit afferent arterioles" Jap.J.Pharmacol.62. 231-237 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideyasu Kiyomoto: "Effects of L-N^G-nitro-arginine,inhibitor of nitric oxide synthesis,on autoregulation of renal blood flow in dogs" Jap.J.Pharmacol.58. 147-155 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Akira Nishiyama: "Effects of semotiadil fumarate(SD-3211)on renal hemodynamics and function in dogs" Eur.J.Pharmacol.218. 311-317 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hong He: "Inhibition of the renin angiotensin system:recent advance" Gardiner-Caldwell Communications(Pacific)Ltd, 10 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuharu Aki: "Nephrology" Spriger Verlage, 9 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tamaki and Y.Abe: "Control of renal circulation-with special reference on nitric oxide." Asia Pacific Journal of Pharmacology. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Aki, T.Tamaki, H.Kiyomoto, H.He, H.Iwao and Y.Abe: "Nitric oxide may participate in V2 vasopressin-receptor-mediated renal vasodilation." J.Cardiovasc.Pharmacol.23. 331-336 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Tamaki, A.Nishiyama, H.Yoshida, H.He, T.Fukui, A.Yamamoto, Y.Aki, S.Kimura, H.Iwao and Y.Abe: "Effects of EXP3174, a non-peptide angiotensin II receptor antagonist, on renal hemodynamics and renal function in dogs." Eur.J.Pharmacol.236. 15-21 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Tamaki, K.Hasui, Y.Aki, S.Kimura and Y.Abe: "Effects of N^G-nitro-arginine on isolated rabbit afferent arterioles." Jpn.J.Pharmacol.62. 231-237 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Masumura, S.Kunitada, K.Irie, S.Ashida and Y.Abe: "A thromboxane A2 synthetase inhibitor retards hypertensive rat diabetic nephropathy." Enr.J.Pharmacol.210. 163-172 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Masumura, S.Miki, Y.Kaife, W.Kitajima and Y.Abe: "Pharmacokinetics and efficacy of carvedilol in hypertensive patients with chronic renal failure and hemodialysis patients." J.Cardiovasc.Pharmacol.19(Suppl.1). S102-S107 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Kiyomoto, H.Matsuo, T.Tamaki, Y.Aki, H.Iwao and Y.Abe: "Effects of L-NG-nitro-arginine, inhibitor of nitric oxide synthesis on autoregulation of renal blood flow in dogs." Jpn.J.Pharmacol.58. 147-155 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.He, H.Masumura, T.Fukui, Y.Aki, S.Kimura, T.Tamaki, H.Iwao and Y.Abe: "Bradykinin contribution to renal vasodilatory and diuretic effects of angiotensin converting enzyme inhibitor in dogs." Inhibition of the renin angiotensin system : recent advance. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Aki, T.Tamaki, H.Kiyomoto, H.Iwao and Y.Abe: "Regulation of renal vascular resistance : studies using pharmacological probes." Nephology. 709-717 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiaki Tamaki: "Control of renal circulation-with special reference on nitric oxide" Asia Pacific Journal of Pharamacology. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasuharu Aki: "Nitric oxide may participate in V_2 vasopressin-receptor-mediated-renal vasodilation" J.Cardiovasc.Pharmacol.23. 331-336 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toshiaki Tamaki: "Effects of EXP3174,a non-peptide angiotensin II receptor antagonist,on renal hemodynamics and renal funkition in doks" Eur.J.Pharmacol.236. 15-21 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toshiaki Tamaki: "Effects of N^G-nitro-arginie on ieolated rabbit afferent arterioles" Jap.J.Pharmacol.62. 231-237 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidemi Masumura: "Athrombxane A_2 synthetase inhibitor retards hypertensive rat diabetic nephropathy" Eur.J.Pharmacol.210. 163-172 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akira Nishiyama: "Effects of semotiadil fumarate(SD-3211)on renal hemodynamics and function in dogs" Eur.J.Pharmacol.218. 311-317 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hong He: "Inhibition of the renin angiotensin system:recent advance" Gardiner-Caldwell Communications(Pacific)Ltd, 10 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hideyasu Kiyomoto: "Effect of L-N^G nitro-arginine,inhibitor of nitric oxide synthesis,on autoregulation of renal blood flow in dogs" Japanese Journal of Pharmacology. 58. 147-155 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hidemi Masumurs: "A thromboxane A_2 synthetase inhibitor retards hypertensive rat diabetic nephropathy" European Journal of Pharmacology. 210. 163-172 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hidemi Masumura: "Pharmacokinetics and efficacy of carvedilol in hypertensive patients with chronic renal failure and hemodialysis patients" Journal of Cardiovascular Pharmacology. 19(Suppl.1). S102-S107 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Akira Nishiyama: "Effects of semotiadil fumarate(SD-3211)on renal hemodynamics and function in dogs" European Journal of Pharmacology. 218. 311-317 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hong He: "Effects of the calcium antagonist manidipine on renal hemodynamics and function in dogs:comparison with nifedipine" Blood Pressure. 1(Suppl.3). 68-74 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tetuo Shoji: "Regional hemodynamic effects of betaxolol,a new selective β_1-blocker,and atenolol in conscious spontaneously hypertensive rats" Japanese Journal of Pharmacology. 60. 253-259 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hong He: "Inhibition of the renin angiotensin system:recent advances" Gardiner-Caldwell Communications (Pacific) Ltd, (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshiaki Tamaki: "Role of adenosine and adenine nucleotides in the biological system" Elsevier Science publishers BV, 726 (1991)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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