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DEVELOPMENT OF SERUM-FABP ASSAY AND IT'S CLINICAL APPLICATION

Research Project

Project/Area Number 04557126
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

ONO Teruo  NIIGATA UNIVERSITY SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (00000927)

Co-Investigator(Kenkyū-buntansha) OKADA Masahiko  NIIGATA UNIVERSITY SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (30018915)
HITOMI Masahiro  NIIGATA UNIVERSITY SCHOOL OF MEDICINE RESEARCH ASSOCIAT, 医学部, 助手 (40218730)
SAKAKIBARA Jun  NIIGATA UNIVERSITY SCHOOL OF MEDICINE RESEARCH ASSOCIAT, 医学部, 助手 (90242403)
FUJII Hiroshi  NIIGATA UNIVERSITY SCHOOL OF MEDICINE ASSOCIATE PROFESS, 医学部, 助教授 (90165340)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1993: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1992: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsFatty Acid-Binding Protein / H-FABP / I-FABP / I-15P / Myocardial Infarct / Serum Mark / L-FABP
Research Abstract

We have succeeded in cloning of two FABP family new members. One is a I-15P from intestine and the other is c-FABP from rat skin. The deduced I-15P cDNA sequence of 127 amino acids was identical that of rat I-15P protein purified from rat intestinal epithelium. Northern blot analysis indicated that the same was deteceted in the ovary suggesting that I-15P or a homologous protein might in volved in the metabolism of steroids in steroid hormone-producing tissues. Another new family member, c-FABP exhibits 50 % identity to myelin P2 protein, adipocyte FABP and heat FABP.
We have developed the assay methods of serum FABPs and tested whether serum FABP is available as a marker of damaged tissues. Serum I-FABP was measured in rats with intestinal ischemic condition. The I-FABP level increased rapidly at 1, 2, 4 and 8 hr after ligation of rat intestine. It also increased at 1 hr after a 30-min transient occlusion and then returned to a normal level. Histological studies supported I-FABP release from necrotic adsorptive epithelial cell. The results suggest that the I-FABP is a useful biochemical maker for intestinal ischemia, particularly in the early reversible phase. The serum H-FABP determinations performed on 9 patitents with acute myocardial infarct (AMI) demonstrated that 4 serum samples obtaining within 1.5 h after heart stroke were within normal level, whereas 5 serum samples obtained with more than 1.5 h after heart stroke were showed elevated H-FABP levels. However, it reached maximum levels faster than serum creatine kinase MB(CK-MB) and creatine phosphokinase (CKB) levels within 6 h after percutaneous transluminal coronary angioplasty (PTCA) treatment. The increase of urinary H-FABP after PTCA treatment is in a marked contrast to urinary CK-MB and CPK which has been scarcely detected. Thus, serum H-FABP is a useful biochemical marker for estimation of recirculation by PTCA treatment rather than the onset of heart stroke.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Kanda,T.: "Intestinal fatty acid-binding protein as a sensitive marker of intestinal ischemia" Dig.Dis.Sci.37. 1362-1367 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe,K.: "Immunohistochemical distribution of heart type fatty acid-binding protein:Immunoreactivity in normal human tissues and in acute myocardial infarct" J.Pathol.170. 59-65 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Amano,O.: "Immunocytochemical localization of rat intestinal 15 kDa protein,a member of cytoplasmic fatty acid binding proteins" Anat.Rec.234. 215-222 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Aono,S.: "Detection of bilirubin binding proteins in the liver on a nitrocellulose membrane" Biomed.Res.13. 69-74 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki,T.: "Establishment of a human hepatoblastoma model in athymic nude mice:Producibility of fatty acid binding protein,α-fetoprotein and α_1-acid glycoprotein" Acta Pathol.Jan.42. 255-261 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii,H.: "Cloning of a cDNA encoding rat intestinal 15 kDa protein and its tissue distribution" Biochem.Biophys.Res.Commun.190. 175-180 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 小野輝夫: "新生化学実験化学講座 脂肪酸結合タンパク質" 東京化学同人, 5 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kanda, T.: "Intestinal fatty acid-binding protein as a sensitive marker of rat intestinal ischemia" Dig.Dis.Sci.37. 1362-1367 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe, K.: "Immunohistochemical distribution of heart type fatty acid-binding protein : Immunoreactivity in normal human tissues and in acute myocardial infarct" J.Pathol.170. 59-65 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Amano, O.: "Immunocytochemical localization of rat intestinal 15 kDa protein, a member of cytoplasmic fatty acid binding proteins" Anat.Rec.234. 215-222 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Aono, S.: "Detection of bilirubin binding proteins in the liver on a nitrocellulose membrane" Biomed.Res.13. 69-74 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki, T.: "Establishment of a human hepatoblastoma model in athymic nude mice : Producibility of fatty acid binding protein, alpha-fetoprotein and alpha_1-acid glycoprotein" Acta Pathol.Jpn.42. 255-261 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii, H.: "Cloning of a cDNA encoding rat intestinal 15 kDa protein and its tissue distribution" Biochem.Biophys.Res.Commun.190. 175-180 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iseki, S.: "Expression and localization of intestinal 15 kDa protein in the rat" Mol.Cell.Biochem.123. 113-120 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe, R.: "Molecular cloning of a cDNA encoding a novel fatty acid binding protein from rat skin" Biochem.Biophys.Res.Commun.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kanda,T.: "Intestinal fatty acid-binding protein as a sensitive marker of intestinal ischemia" Dig.Dis.Sci.37. 1362-1367 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Watanabe,K.: "Immunohistochemical distribution of heart type fatty acid-binding protein:Immunoreactivity in normal human tissues and in acute myocardial infarct" J.Pathol.170. 59-65 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Amano,O.: "Immunocytochemical localization of rat intestinal 15 kDa protein,a member of cytoplasmic fatty acid binding proteins" Anat.Rec.234. 215-222 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Aono,S.: "Detection of bilirubin binding proteins in the liver on a nitrocellulose membrane" Biomed.Res.13. 69-74 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Suzuki,T.: "Establishment of a human hepatoblastoma model in athymic nude mice:Producibility of fatty acid binding protein,α-fetoprotein and α_1-acid glycoprotein" Acta Pathol.Jan.42. 255-261 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujii,H.: "Cloning of a cDNA encoding rat intestinal 15 kDa protein and its tissue distribution" Biochem.Biophys.Res.Commun.190. 175-180 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小野輝夫: "新生化学実験化学講座 脂肪酸結合タンパク質" 東京化学同人, 5 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tatsuo Kanda: "Intestinal fatty acid binding protein as as sensitive marker of intestinal ischemia" Digestive Disease and Sciences. 37. 1362-1367 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroshi Fujii: "Cloning of a cDNA encoding rat intestinal 15 kDa protein and its tissue distribution" Biochem.Biophys.Res.Commun.190. 175-180 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sachiko Aono: "Detection of bilirubin-binding proteins in the liver on a nitrocellulose membrane" Biomedical Research. 13. 69-74 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshimitsu Suzuki: "Establishment of a human hepatoblastoma model in athymic nude mice:Producibility of fatty acid binding protein, α -fetoprotein,and α_1 -acid glycoprotein" Acta Pathologica Japonica. 42. 255-261 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Osamu Amano: "Immunocytochemical localization of rat intestinal 15 kDa protein,a member of cytoplasmic fatty acid binding proteins" Anatomical Record. 234. 215-222 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小野 輝夫: "トピックスFABP" 検査と技術. 20. 1118-1119 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小野 輝夫: "実験生化学講座第4巻脂肪酸結合タンパク質" 東京化学同人, 5 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小野 輝夫: "基礎科学講座脂肪酸結合蛋白" 広川書店, 3 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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