| Project/Area Number |
04558025
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KAWABATA Shun-ichiro KYUSHU UNIVERSITY, FACULTY OF SCIENCE, RESEARCH ASSOCIATE, 理学部, 助手 (90183037)
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| Co-Investigator(Kenkyū-buntansha) |
NISHINO Norikazu KYUSHU INSTITUTE OF TECHNOLOGY, FACULTY OF ENGINEERING, PROFESSOR, 工学部, 教授 (40145165)
IWANAGA Sadaaki KYUSHU UNIVERSITY, FACULTY OF SCIENCE, PROFESSOR, 理学部, 教授 (90029942)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1993: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | Processing protease / Metalloendopeptidase / Synthetic substrate / Fluorogenic substrate / Metalloprotease / プロペプチド酵素 / プロセシング / メタルプロテアーゼ / 蛍光性ペプチド基質 / 合成阻害剤 |
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| Research Abstract |
Ten fluorogenic synthetic substrates of Abz (2-aminobenzoyl)-peptide-Dna(2,4-dinitro-anilinoethylamide) were newly synthesized and tested to find specific substrates for rabbit liver microsomal metalloendopeptidase (MEP) as shown in Table. Among these substrates, Abz-ARVRRANS-Dna or Abz-ARVTRANS-Dna was found to be the most sensitive substrates for MEP.The assay method using these peptide substrates enabled us to detect MEP activity in an aliquot (20-50 mul) of the crude extract.
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