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Development of a newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic field

Research Project

Project/Area Number 04559006
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIYAKOSHI Junji  Kyoto University, Faculty of Medicine, Lecturer, 医学部, 講師 (70121572)

Co-Investigator(Kenkyū-buntansha) TAKEBE Hiraku  Kyoto University, Faculty of Medicine, Professor, 医学部, 教授 (10028318)
TSUKADA Toshihiko  Kyoto University, Faculty of Medicine, Research Associate, 医学部, 助手 (10207334)
HIRAOKA Masahiro  Kyoto University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (70173218)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 1993: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥8,400,000 (Direct Cost: ¥8,400,000)
KeywordsExtremely Low Frequency Magnetic Field / Cultured Mammalian Cells / Gene Expression / beta-galactosidase / Signal Transduction / Cyclic AMP / Protein Kinase C / プロテインカイネースC / 磁石 / 放射線感受性 / 情報伝達
Research Abstract

To examine the biological effects of extremely low frequency magnetic field (ELFMF), we have designed and manufactured a new equipment for long-term and high density exposure of cells to ELFMF.The ELFMF exposure system consists of a generator of magnets with a bult-in CO_2 incubator, an alternating current power supply, a gas compressor and thermocontroller for the incubator, and a cooling unit for the magnets. The maximum magnet flux density on the exposure area of the incubator is 500 mT (tesla) at a current of 556 Arms at 50 Hz. The effect of ELFMF on gene expression was investigated using PCVG cells which were transfected with the beta-galactosidase (beta-gal.) gene expression plasmid in the parental PC12 cells. The activity of beta-gal. was very low without stimulation. The treatment with forskolin (2muM) enhanced the beta-gal. activity. The activity was further enhanced by the combined exposure with forskolin and 400 mT ELFMF.The stimulation by forskolin and TPA (25ng/ml) induced 2.5-fold increase in the beta-gal. activity. The activity was further enhanced under exposure of ELFMF.From these results, the exposure of ELFMF may stimulate the intracellular signal transduction resulting in the beta-gal. expression in PCVG cells.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Junji Miyakoshi: "A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic field." Journal of Radiation Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Junji Miyakoshi, Shuji Ohtsu, Junko Tatsumi-Miyajima, and Hiraku Takebe: "A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic field." Journal of Radiation Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Junji Miyakoshi: "A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic field." Journal of Radiation Research. (in press).

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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