|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1993 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1992 : ¥1,200,000 (Direct Cost : ¥1,200,000)
1. Hydrophobic Interaction around beta-IononeRing of Chromophores
5,6-Dihydro-, 7,8-dihydro-, alpha-, and 3,4-dehydroretinals were synthesized Pigment formation of the retinal analogs with aporetinochrome resulted in apparent completion with loose hydrophobic interaction around the beta-ionone ring.
2. Opsin Shifts of Retinal Analogs without beta-Ionone Ring
The opsin shift of the structurely linear analog, 3,7-dodecapentaenal showed 1400 cm^<-1> smaller value of the neative one, 2400 cm^<-1>.
3. The Conformation of Retinochrome Chromophore in 6S-Bond
With 6S-cis locked retinal analog, the pigment were formed as in native retinochrome formation, while 6S-trans locked analog gave no pigment.
4. Regiospecific Photoisomerization of Retinal Analogs
Photoisomerization of 3,7-DDP with aporetinochrome regiospecifically gave 11-cis typed isomer, whereas 3,7-DDT did not show the high selectivity, 43%.
5. CD Spectra of 6S-Cis Locked Retinal Analogs
The retinochrome analogs with 8,18-methano- and ethanoretinal gave intense and negative beta-band compared with positive alpha-band.
11-Methyl-, 13-demethyl-11-methyl-, and 9, 13-didemethyl- 11-methylretinals were synthesized. The conformation of all-trans 11-methylretinal were determined to be 10,12-di-S-cis. Photoisomerization of these analogs with aporetinochrome furnished corresponding 11-cis isomers in more than 90% regioselectivity.