Molecular mechanisms of the bacterial aggregation ; (Interaction of the bacterial cell surface polysaccharide responsible for the aggregation)
Grant-in-Aid for Scientific Research (C).
|Allocation Type||Single-year Grants|
|Research Institution||The University of Tokyo|
TAGO Yoshitaka The University of Tokyo, Institute of Molecular and Cellular Biosciences, Instructor, 分子細胞生物学研究所, 助手 (70111573)
|Project Period (FY)
1992 – 1993
Completed(Fiscal Year 1993)
|Budget Amount *help
¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1993 : ¥700,000 (Direct Cost : ¥700,000)
|Keywords||Bacterial aggregation / Conformation of polysaccharide / Ionic strength / Molecular weight of polysaccharide / Interaction of polysaccharide / Antiparalell / Double strands / Cell surface polysaccharide|
The cell surface polysaccharide (MPS) responsible for cell aggregation in Pseudomonas sp.no.12 was linked to a protein(Anchor protein : AP) in o-glycosidic manner. The protein(AP) was hydrohobic and associated to peputidoglycan. MPS molecules were interacted each other. This interaction related to ionic strength of the circumstance and resulted to induce cell aggregation. This research was about conformational change of MPS regarding molecular weight of MPS and ionic strength and the role of branched sugar(GlcNAc). The following results were obtained.
(1) MPS molecules formed antiparallel double strands conformation.
(2) The MPS molecules formed the double strands conformation could not interact other MPS molecules.
(3) The MPS liberated the branched GlcNAc could not form the conformation.
(4) N-deacetylated MPS could not form the double strands conformation.
(5) The specificity of the formation of double strands conformation was very high and no polysaccharide could interfere the formation.
(6) The strength of the interaction of the MPS was proportional to the concentration of MPS.
(7) The strength of the interaction of MPS molecules was proprtonal to the MW3/2 of MPS.
(8) High concentration of MPS induced to complete the formation of double strands under low ionic strength, and high ionic strength condition was needed to form the conformation in the case of low concentration of MPS.
(9) High molecular weight of MPS could form the conformation under low ionic strength, and under low concentration of MPS.
Research Output (9results)