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Structure and function of gamma-polyglutamate gene in Bacillus subtilis(natto)

Research Project

Project/Area Number 04660123
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

HARA Toshio  Kyushu Univ., Fac.of Agr., Associate Prof., 農学部, 助教授 (50117222)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBacillus subtilis(natto) / gamma-Polyglutamate / Biopolymer / gamma-PGA synthetase / gamma-PGA depolymerase / gamma-ポリグルタミン酸 / gamma-ポリグルタミン合成酵素 / gamma-ポリグルタミン酸分解酵素 / 納豆菌プラスミド / γ-グルタミルトランスペプチダーゼ / 構造と機能解析 / 分子進化 / ゲノムライブラリー / DNA複製開始領域 / Bacillus licheniformis
Research Abstract

Natto, or non-salty fermented soybeans, is an aging product by the biological action of Bacillus subtilis(natto)grown on the steamed soybeans. Natto is covered with a viscous, sticky polymer that formed long, thin threads when touched. The main component of the viscous material is gamma-polyglutamate(gamma-PGA). The genetic character of high gamma-PGA productivity in B.subtilis(natto)was transferred to B.subtilis Marburg 168, which cannot produce gamma-PGA.Treatment of B.subtilis(natto)with acridine orange resulted designated pUH1 was detected in B.subtilis(natto)strain, but the stgderivatives were missing the 5.8-kb plasmid. The specific gene of natto plasmid pUH1, which is responsible for gamma-PGA production, has been cloned and the nucleotide sequence determined.
There are many kinds of natto-like non-salty fermented soybean foods in Monsoon Asia. These natto-like fermented foods are considered to have streamed from the same cultural roots, which may be termed as "Natto Triangle". We might probably consider the center of this area as the roots of "Biotechnology" of our region. The natto plasmids were detected from the essential organisms for fermentation isolated from these natto-like fermented soybeans the natto plasmids, and by constructing the genealogical tree of these plasmids.
Moreover, the unbalanced consumptions of natto in Japan would be discussed, including the origin of the Japanese.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] T.Hara,S.Ogata and S.Ueda: "Plasmid distribution in γ-polyglutamate-producing Bacillus strains isolated from “dan-douchi",a“natto"-like non-salty fermented soybeans food in China." Journal of General Applied Microbiology. 39. 75-82 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 原 敏夫: "納豆のルーツを求めて" 第一回納豆学術会議講演集. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara,N.Iwamoto and M.Orima: "Plasmid distribution in γ-polyglutamate-producing Bacillus strains isolated from “natto"-like non-salty fermented soybeans food,“kinema",in China." Journal of General Applied Microbiology. (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara,S.Nagatomo,S.Ogata and S.Ueda: "The DNA sequence of γ-glutamyltranspeptidase gene of Bacillus subtilis(natto)plasmid pUH1." Applied Microbiology and Biotechnology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara,K.Nakajima,H.Saito,A.Ishizaki,S.Ogata and S.Ueda: "Sequence analysis of replication origin of plasmid pLS11 of Bacillus subtilis." Bioscience,Biotechnology and Biochemistry. 56. 223-227 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara,S.Yasuda,S.Ogata,E.Soeda and S.Ueda: "Complete nucleotide sequence of Bacillus subtilis(natto)plasmid responsible for γ-polyglutamate synthesis." Journal of Faculty of Agriculture,Kyushu University. 36. 209-218 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara, N.Iwamoto and M.Orima: "Plasmid distribution in gamma-polyglutamate-producing Bacillus strains isolated from "natto"-like non-salty fermented soybeans food, "kinema", in China." J.Gen.Appl.Microbiol.(Submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshio Hara: "Roots of "natto"-like non-salty fermented soybeans" Proceeding of International Conference on "Natto". (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara, S.Ogata and S.Ueda: "Plasmid distribution in gamma-polyglutamate-producing Bacillus strains isolated from "dan-douchi", a "natto"-like non-salty fermented soybeans food in China" J.Gen.Appl.Microbiol.39. 75-82 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara, S.Nagatomo, S.Ogata and S.Ueda: "The DNA sequence of gamma-glutamyltranspeptidase gene of Bacillus subtilis(natto)plasmid pUH1." Appl.Microbiol.Biotechnol.37. 211-215 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara, K.Nakajima, H.Saito, A.Ishizaki, S.Ogata and S.Ueda: "Sequence analysis of replication origin of plasmid pLS11 of Bacillus subtilis." Biosci.Biotech.Biochem.56. 223-227 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara, S.Yasuda, S.Ogata, E.Soeda and S.Ueda: "Complete nucleotide sequence of Bacillus subtilis(natto)plasmid responsible for gamma-polyglutamate synthesis." J.Fac.Agr., Kyushu Univ.36. 209-218 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Hara.S.Ogata&S,Ueda: "Plasmid distribution in g-polyglutamate-producing Bacillus strains isolated from “dan-douchi",a“natto"-like non-salty fermented soybeans food in China." Journal of General Applied Microbiology. 39. 75-82 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Hara,K.Nakajima,H.Saito,A.Ishizaki,S.Ogata and S.Ueda: "Sequence analysis of replication origin of plasmid pLS11 of Bacillus subtilis IFO 3022." Bioscience,Biotechnology and Biochemistry. 56. 223-227 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Hara,S.Yasuda,S.Ogata,E.Soeda and S.Ueda: "Complete nucleotide sequcnce of Bacillus subtilis(natto)plasmid responsible for γ-polyglutamate synthesis." Journal of Faculty of Agriculture,Kyushu University. 36. 209-218 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Hara,S.Nagatomo,S.Ogata and S.Ueda: "DNA sequence of γ-glutamyltranspeptidase gene of Bacillus subtilis(natto)plasmid pUH1." Applied Microbiology and Biotechnology. 37. 211-215 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Hara,S.Ogata and S.Ueda: "Plasmid distribution in γ-polyglutamate-producing Bacillus strains isolated from “Dan-douchi",“Natto"-like non-salty fermented soybean food in Chin" Journal of General Applied Microbiology. 39. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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