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Postsynaptic Mechanism of Long-Term Potentiation

Research Project

Project/Area Number 04670706
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionNagoya City University

Principal Investigator

SUZUKI Tatsuo  Nagoya City University. Medical School Associate Professor, 医学部, 助教授 (80162965)

Co-Investigator(Kenkyū-buntansha) TANAKA Ryo  Nagoya City University. Medical School Professor, 医学部, 教授 (90094383)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsCalmodulin kinaseII / Protein kinase C / autophosphorylation / long-term potentiation / calpain / calcium / tramslocation / カルモジュリンキナーゼII / Cキナーゼ
Research Abstract

1. A specific substrate for protein kinase C, distinct from contaminating myelin basic protein, was found in the postsynaptic density fraction prepared form rat cerebrum.
2. The cubstrate (250 kDa) of calmodulin kinase II associated with postsynaptic density was found to be related to P _<400> protein, and that of 200 kDa related to myosin.
3. We prepared an antibody that stecifically reacts with calmodulin kinase II autophosphorylated at Thr286/287 site. The antibody is a powerful tool to detect an active form of calmodulin kinase II both in vivo and in vitro.
4. Immunohistochemical analysis was done on rat brains using sntibody specifically reacting autophosphrylated CaMKII.Neurons in all subregions of hippocampus were stained in the rat in which kindling was established. Especially the somas and dendrites of CA1 region were strongly immunoreactive. The results suggest that activation of aclmodulin kinase II is involved in the development and maintenance of kindling.
5. We found elevation of Ca^<2+>-independent protein kinase C activity in the supermatant fraction and, concomitantly, limited proteolysis of protein linase C in the particulate fraction of rat hippocampi in which lang-term potentiation was induced. The experiment suggested that proteolytic conversion of protein kinase C may contribute to the maintenance of long0term patentiation. We also suggest that calpain is redponsible for the production of Ca_<2+>-indepadent form of protein kinase C.
6. We found that calmodulin kinase II, both alpha and beta/beta', artificially translocate into postsynaptic density after decapitation. The experiment suggested that the concentration of calmodulin kinase II in the PSD in vivo is not high as has been believed previously.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Suzuki,T.: "Antibody reacting specifically to the Thr^<286>-autophosphorylated α-subunit of the Ca^<2+>/calmodulin dependent protein kinase II." Proc.Natl.Acad.Sci.U.S.A.89. 109-113 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki,T.: "The Presence of 17K.Mr.Protein,a major specific substrate for kinase C,found in the Triton-insoluble fraction of synaptosome prepared from rat brain." Neurochem.Res. 17. 1091-1097 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Abe-Dohmae,S.: "Ca^<2+>-binding proteins in rat synaptic fractions surveyed by ^<45>Ca^<2+> overlay method." Brain Res.598. 225-232 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki,T.: "Calpain may produce Ca^<2+>-independent form of kinase C in long-term potentiation." Biochem.Biophys.Res.Commun.189. 1515-1520 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.SUZUKI: "Characterization of protein kinase C activities in postsynaptic density fractions prepared from cerebral cortex,hippocampus and cerebellum." Brain Res.619. 69-75 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Zhou,X.-R.: "Amygdala kindling activates the phosphorylation of Ca^<2+>/calmodulin-dependent protein kinase II in rat hippocampus." Neurosci.Lett.(in Press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inamura,K.: "Phosphorylation state of calcium calmodulin kinase II(CaM-K II)in ischemia and recirculation in the rat brain." J.of Autonomic Nervous System. (in Press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 鈴木龍雄: "シナプス後肥厚部における長期増強発現のメカニズム" 薬物・精神・行動. 12. 135-140 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 鈴木龍雄: "記憶,学習とタンパク質リン酸化反応" 生体の科学. 44. 710-719 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki,T.: "Protein kinases involved in the expression of long-term potentiation." Int.J.Biochem.(in Press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki,T.: "P_<400> Protein is one of the major substrates for Ca^<2+>/calmodulin-dependent protein kinase II in the postsynaptic density-enriched fractions isolated from rat cerebral cortex" Neurochem.Int.20. 61-67 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Suzuki, K.Okumura-Noji, A.Ogura, Y.kudo, and R.Tanaka: "Antibody reacting stecifically to the Thr-286-autophosphorylated alpha-subunit of the Ca2_2/caimodulin-dependent protien kinase II." Proc. Natl. Acad. Sci. U.S.A.89. 109-113 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Suzuki, Abe-Dohmae, S., and R.Tanaka: "The presence of 17k Mr protein, a major stecific substrate for kinase C, found in the Triton-insoluble fraction of sysnptosome prepared from rat brain." Neurochem. Res.17. 1091-1097 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Abe-dohmae, T.Suzuki, and R.Tanaka: "Ca2_+-binding proteins in rat synaptic fractions surveyed by 45Ca2_+ overlay method." Brin Res.598. 225-232 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Suzuki, K.Okumura-Noji, A.Ogura, R.Tanaka, K.Nakaumura, and Y.Kodo: "Calpain may produce Ca2_+-independent form of kinase C in long-term potentiation" Biochem. Biophys. Tes. Commun.189. 1515-1520 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Suzuki, Abe-Dohmae, and R.Tanaka: "P400 protein is one of the major substrates for Ca2_+/calmodukin-dependent protein kinase II in the postsynaptic density-enriched fractions isolated from rat cerebral cortex." Neuchem. Int.20. 61-67 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Suzuki, K.Okumura-Noji, R.Tanaka, A.Ogura, k. Nakamura, Y.Kudo, and T, Tada: "Characterization of protein kinase C activities in postsynaptic density fractions prepared from cerebral cortex, hippocampus and cerebellum." Brain Res.619. 69-75 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Zhou, X-R., Suzuki, T., Shimizu, H., and Nishino, H.: "amygdala kindling activates the phosphorylation of Ca^<2+>/calmodulin-dependent protein kinase II in rat hippocampus." Neurosci. Lett.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Suzuki, K.Okumura-Noji, R.Tanaka, and T.Tada: "Rapid translocation of alpha-subunit if cytosolic Ca2+/calmodulin-dependent protein kinase II to particulate fraction after decapitation. (submitted)." (submtted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inamura, K., Sakamoto, S., katsumata, T., Ichizeki, H., Suzuki, T., Katayama, Y.and Terashi, A.: "Phosphorylation state of calcium calmodulin kinase II (CaM-KII) in ischemia and recirculation in the rat brain." J.of Autonomic nervous System.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki, T.: "Protein kinases involved in the expression of long-term potentiation." Int. J.Biochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary

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Published: 1992-04-01   Modified: 2016-04-21  

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