Development of the Screening System for Adrenal Hypertension
Grant-in-Aid for Scientific Research (C).
|Research Institution||Hokkaido University|
CHIBA Hitoshi Hokkaido university Medical Hospital, Lecturer, 医学部・附属病院, 講師 (70197622)
|Project Fiscal Year
1992 – 1993
Completed(Fiscal Year 1993)
|Budget Amount *help
¥1,900,000 (Direct Cost : ¥1,900,000)
Fiscal Year 1993 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1992 : ¥1,000,000 (Direct Cost : ¥1,000,000)
|Keywords||Primary aldosteronism / Glucocorticoid-remediable aldosteronsim / 18-hydroxycortisol / Mass-screening / Pheochromocytoma / Vanillyl mandelic acid / 原発性アルドステロン症 / 糖質コルチコイド反応性アルドステロン症 / マススクリーニング / 褐色細胞腫 / vanillyl mandelic acid / vanillylmanderic acid / 18‐hydroxycortisol / 化学発光 / イムノアッセイ|
A) Reseacrh of the screening system for primary aldosteronism and glucocorticoid-remediable aldosteronism
1) antomated enzyme immunoassay, using polyclonal antibody-coated tube and peroxidase-labeled 18-hydroxycortisol, was established. Following is the results obtained by the assay :
a) We confirmed that dry urin spot on the filter paper can be assayd by this method.
b) We confirmed that spot-time urine is useful for the diagnosis of primary aldosteronism.
c) We have succeeded in screening an affected member in a pedigree of glucocorticoid-remediable aldosteronism.
2) automated chemiluminescence immunoassay, using monoclonal antibody-coated magnet support and acridinum ester-labeled 18-hydroxycortisol, was established. Following is the results obtained by the assay :
a) Assay time was shortened from 3 h to 15 min, and senseitivity was improved 10-fold.
b) This system including the monoclonal antibodies has been submitted for patent.
B) Research of the screening system for pheochromocytoma
The conjugate of vanillylmandelic acid and bovine serum albumin was immunized in mouse, and monoclonal antibodies with high specificity and affinity have been established.
Chemiluminescence immunoassay is being prepared.
Research Output (20results)