IMMUNOHISTOCHEMICAL EXAMINATION OF THE INNER EAR
Project/Area Number |
04671031
|
Research Category |
Grant-in-Aid for General Scientific Research (C)
|
Allocation Type | Single-year Grants |
Research Field |
Otorhinolaryngology
|
Research Institution | NIIGATA UNIVERSITY |
Principal Investigator |
IGARASHI Shuichic NIIGATA UNIVERSITY SCHOOL OF MEDICINE DEPARTMENT OF OTOLARYNGOLOGY ASSOCIATED PROFESSOR, 医学部, 助教授 (90103499)
|
Co-Investigator(Kenkyū-buntansha) |
SEKI Satoshi NIIGATA UNIVERSITY SCHOOL OF MEDICINE DEPARTMENT OF OTOLARYNGOLOGY MEDICAL STAFF, 医学部・附属病院, 医員
長場 章 新潟大学, 医学部附属病院, 医員
|
Project Period (FY) |
1992 – 1993
|
Project Status |
Completed (Fiscal Year 1993)
|
Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
Keywords | inner ear / immunohistochemistry / decalcified specimen / neuropeptide / Minamata disease / calcium-binding protein |
Research Abstract |
1) Immunohistochemical investigation of neuropeptide using decalcified specimen Several neuropeptides including substance P(SP) are regarded as central nervous system neurotransmitters or neuromodulators, and thair localization in the inner ear has been investigated immunohistochemically. But most such studies have been performed by the immunofluorescence method using frozen sections. We investigated the localization of certain neuropeptides in decalcified inner ear tissue immunohistichemically and the results were then compared with the results obtained by using frozen sections to determine whether immunohistochemical studies of decalcified specimens are possible, and we concluded that they are possible. 2) Mercury deposition and its relationship to inner ear function in methylmercury-poisoned rats. Mercury deposition and its relationship to the inner ear function in methylmercury-poisoned rats were examined. Mercury deposits were found in parts of the vestibular nerves, cochlear nerves,
… More
spiral ganglion and stria vascularis. In the vestibule, slight mercury deposition was observed in the acoustic maculae and in the cochlea, only one instance of mercury deposit in the organ of Corti was detected. The effect of mercury on inner ear function was also assessed on the basis of immunohistochemical localization of substance P, which may be a neurotransmitter in the inner ear, however, no pathological changes were detected. These findings suggest that methylmercury deposition has little effect on the inner ear. 3)Distribution of calcium binding proteins in the ear. Distribution of three types of calcium-binding protein (spot-35 protein (S-35), calbindin (CaB) and palvalbumin (PaV) were examined immunohistochemically.Immunoreactivity of S-35 and CaB was found in the outer hair cells and in vestibular sensory cells situated at the top of the ampulla and in some cells in the macula. These results indicate that S-35 and CaB play important roles in the special kinds of chemoreceptor cells. Less
|
Report
(3 results)
Research Products
(11 results)