| Project/Area Number |
04671100
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
FUKUI Kazuhiro Okayama University, Dental School, Professor, 歯学部, 教授 (70034171)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Hiroyuki Okayama University, Dental School, Associate Professor, 歯学部, 助教授 (80168947)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Streptococcous cricetus / glucosyltransferase / cloning GTF gene / water-soluble glucan synthesis / mutans streptococci / dextransucrase / glucosyltransferase-S / α-1,6 glucan synthase / GTF-S gene |
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| Research Abstract |
Streptococcus cricetus has four kinds of glucosyltransferase (GTFs), i.e.GTF-I,GTF-S,GTF-SI and GTF-T.These enzymes co-operatively synthesize adhesive water-insoluble glucan from sucrose, which contributes firm adherence of growing organisms to smooth dental surfaces.
In this study, the genes encoding glucosyltransferase reponsible for water-soluble glucan synthesis (GTF-S and GTF-T) of S.cricetus HS-6 were cloned. THe sequence of GTF-S genes from S.cricetus and S.downei were compered and show a high degree of homology. There is a 73.9% homology of nucleotides in the genes, and 77.4% homology of amino acids in the deduced protein sequences. In comparison of GTF-T genes with S.cricetus and S.sobrinus, there is 79.7% homology in the genes, and 83.5% homology in the deduced protein sequences.
The availability of recombinant E.coli carrying the these genes will greatly facilitate purification of the enzymes for biochemical studies.
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