Inhavition mechanism of oral cancer invasion
Grant-in-Aid for Scientific Research (C).
|Research Institution||Osaka university|
SHIRASUNA Kanemitsu Osaka University Faculty of Dentistry, 1st Department of Oral and Mxillofacial Surgery, Associate Professor, 歯学部, 助教授 (30093420)
|Project Fiscal Year
1992 – 1993
Completed(Fiscal Year 1993)
|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1993 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 1992 : ¥1,000,000 (Direct Cost : ¥1,000,000)
|Keywords||Salivary gland tumor / Invaion / Extracellular matrix / Protease / Protease inhibitor / Matrix degradation / Motility / Integrin / 唾液腺腫瘍 / 癌浸潤 / 細胞外基質 / プロテアーゼ / プロテアーゼインヒビター / 細胞外基質分解 / 細胞遊走 / インテグリン / 口腔癌 / 癌浸潤機構 / 細胞外基質分解酵素 / モティリティ因子|
In order to study invasion mechanism of oral cancer, I mainly used ACCS line derived from adenoid cystic carcinoma (ADCC).
The findings obtained from the present projedt are :
1. ACCS cells produce uPA, MMP-2 and MMP-9, and can degrade condiderable amount of ECM elaborated by normal mesenchymal cells, through mainly uPA-plasmin cascade.
ACCS cells vigorously produce extracellular matorix (ECM), including fibronectin, laminin, and type IV collagen. the ACCS-ECM is resistant to degradation by its own proteolytic enzymes because of the presence of abundant PAI-1 in the ECM.
ACCS-ECM promotes haptotactic motility of ACCS cells.
Chemotactic response of ACCS cells to laminin and type cancer cells suggesting relation to a special proclivity of ADCC cells to invade nerve and endothelial sheaths.
EGF causes enhancement of cell migration and uPA production.
Dezamethasone causes inhibition of cell migration and uPA production but enhancement of PAI-1
ECM-induced migration is inhibited by monoclonal antibodies against integrins.
A factor purified from conditioned medium with human fibroblasts, which is heaprin-unbinded protein, enhances chemotactic migration of cancer cells.
Another one from fibroblasts enhances protease production by cancer cells.
Research Output (7results)