Roles of the protein kinases on platelet functions
Grant-in-Aid for Scientific Research (C).
|Research Institution||University of Tokyo|
HASHIMOTO Yoshiaki Medicine, Internal Medicine, Assistant Prof., 医学部(病), 助手 (40172879)
KINOSHITA Makoto Medicine, Internal Medicine, Assistant Fellow, 医学部(病), 医員 (70186295)
徳永 一之 東京大学, 医学部(病), 医員
TOKUNAGA Kazuki Medicine, Internal Medicine, Assistant Fellow
|Project Fiscal Year
1992 – 1993
Completed(Fiscal Year 1993)
|Budget Amount *help
¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1993 : ¥1,000,000 (Direct Cost : ¥1,000,000)
Fiscal Year 1992 : ¥1,200,000 (Direct Cost : ¥1,200,000)
|Keywords||platelet aggregation / ATP release / myosin light chain kinase / protein kinase C / wortmannin / 血小板凝集 / ATP放出 / ミオシン軽鎖燐酸化酵素 / C-キナーゼ|
We examined the roles of the protein kinases in platelet aggregation and release.
1.Platelets : Platelet rich plasma and washed platelets were prepared from bloods of healthy volunteers.
2.Measurement of platelet responses : Platelet aggregation and ATP release were monitored using a CAF-100 Ca^<2+> analyzer. ATP release was measured by the luciferin-luciferase reaction.
1.The roles of myosin light chain kinase.
The specific inhibitor of myosin light chain kinase wortmannin inhibited ADP-induced platelet aggregation with no effect on their shape change.
2.The roles of protein kinase C.
The specific inhibitor of protein kinase C PKC-I did not affect much U46619-induced platelet aggregation and ATP release. However the inhibition of platelet aggregation with GRGDS prevented ATP release in the presence of PKC-I.On the other hand, ATP release was not seen in the presence of PKC-I in washed platelets.
1.It was suggested that myosin light chain kinase activation is a prerequisite for ADP-induced platelet aggregation, but not for changes in their shape.
2.It was suggested that there are protein-kinase C-dependent and -independent mechanisms for ATP release by human platelets and that activation of the latter mechanism may depend on aggregation and plasma factors.
Research Output (12results)