HIRAGA Shoju Osaka University Medical School, Assistant, 医学部, 助手 (40243232)
ARITA Norio Kinki University Medical School, Assistant, 医学部, 助手 (80159508)
HAYAKAWA Toru Osaka University Medical School, Professor, 医学部, 教授 (20135700)
|Budget Amount *help
¥2,000,000 (Direct Cost : ¥2,000,000)
Fiscal Year 1993 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 1992 : ¥900,000 (Direct Cost : ¥900,000)
We have previously demonstrated that human malignant glioma cells, T98G, secrete a factor taht stimulates the motility of the producer cells (J Neurosurg 73 : 881-888, 1990). The glioma-derived motility factor (GMF) has been purified to homogeneity from the serum-free conditioned medium of T98G by gelatin affinity chromatography and heparin affinity-, DEAE anion exchange-, hydroxyapatite-, gel permiation- and sulfopropyl high performance liquid chromatography. The GMF consisted of two molecular species, GMF-I and GMF-II, whose molecular mass were 145 kDa and 165 kDa, respectively. The physicochemical characteristics of these two GMFs are similar with respect to amino acid composition. GMF-I and GMF-II both stimulated the migration of T98G cells in a concentration-dependent manner, and the activity of GMF-I was 5 to 10 times as strong as that of GMF-II.Checcurboard analysis demonstrated that the GMFs had not only a chemotactic effect but also a chemokinetic effect on T98G cells, both of which showed high invasiveness in an invitro invation assay with reconstituted basement membrane, Matrigel, migrated to the GMFs with great intensity, while A172 and 9L glioma cells and normal glial cells, all of which weakly infiltrated the Matrigel barrier, migrated to the GMFs with much less intensity. These results indicate that migratory response of glioma cells to the GMFs correlates well with invasiveness, suggesting an important role of the GMFs in the process of gloma cell invasion.