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魚類のサイトカイン遺伝子のクローニングと構造解析

Research Project

Project/Area Number 04F04210
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field General fisheries
Research InstitutionUniversity of Miyazaki

Principal Investigator

酒井 正博  宮崎大学, 農学部, 教授

Co-Investigator(Kenkyū-buntansha) RAM SAVAN  宮崎大学, 農学部, 外国人特別研究員
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsサイトカイン / フグ / ゼブラフィッシュ / 自然免疫応答 / インターフェロン / TNF / インターロイキン10
Research Abstract

1 魚類からインターロイキン6,17,22,26の遺伝子を初めてクローニングに成功した。
2 魚類では従来1種類しかないとされていた腫瘍壊死因子(TNF)の遺伝子がほ乳類と同じように染色体上で複数並んでいることを発見した。
3 魚類ではほ乳類と異なり、インターフェロンガンマの遺伝子が染色体上で2つタンデムに並んでいることを見いだした。
4 インターロイキン2のファミリーとしてほ乳類で知られているインターロイキン2,15,21以外に魚類では、新しいタイプの遺伝子が存在することを発見した。
5 これらのサイトカイン遺伝子を用いて、魚類の免疫賦活剤の効果判定が可能なことを示した。

Report

(2 results)
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (12 results)

All 2006 2005 2004

All Journal Article (12 results)

  • [Journal Article] Genomics of fish cytokines2006

    • Author(s)
      Savan R., Sakai, M.
    • Journal Title

      Comparative Physiology and Biochemistry (D) 1(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] An unexpected discovery of two interferon gamma like genes along with interleukin(IL)-22 and IL-26 from teleost2006

    • Author(s)
      Igawa, D., Sakai, M., Savan, R.
    • Journal Title

      Molecular Immunology 43(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification, cloning and characterization of interleukin-17 and its family from zebrafish2006

    • Author(s)
      Gunimaladevi, I., Savan, R.Sakai, M.
    • Journal Title

      Fish and Shellfish Immunology 20(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of expressed genes on carp (Cyprinus carpio) head kidney cells after in vitro treatment with immunostimulants2005

    • Author(s)
      Sakai, M., Kono, T., Savan, R.
    • Journal Title

      Developmental Biology 121

      Pages: 45-52

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A novel tumor necrosis factor (TNF) gene present along with TNF alpha in tandem on the same chromosome in teleost2005

    • Author(s)
      Savan.R., Kono, T., Igawa, D., Sakai, M.
    • Journal Title

      Immunogenetics 57

      Pages: 140-150

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Characterization and expression analysis of an interleukin 6 homologe in the Japanese pufferfish, Fugu rubripes2005

    • Author(s)
      Bird, S., Zou, J., Savan, R., Kono, T., Sakai, M., Woo, J., Secombes, C.J.
    • Journal Title

      Development and Comparative Immunology 29

      Pages: 775-789

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Diversification in MHC class II invariant chain like proteins among fishes2004

    • Author(s)
      Sakai, M., Savan, R., Kuragazaki, H., Aman, A.
    • Journal Title

      Journal of Applied Ichthyology 20

      Pages: 252-257

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Presence of multiple isoforms of TNF alpha in carp (Cyprinus carpic L.) : its genomic and expression analysis2004

    • Author(s)
      Savan, R., Sakai, M.
    • Journal Title

      Fish & Shellfish Immunology 17

      Pages: 87-94

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Non-specific cytotoxic cell receptor (NCCRP) 1 type gene in tilapia (Oreochromis niloticus) : its cloning and analysis2004

    • Author(s)
      Ishimoto, Y., Savan, R., Endo, M., Sakai, M.
    • Journal Title

      Fish & Shellfish Immunology 16

      Pages: 163-172

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of a new C type lectin from common carp Cyprinus carpio.2004

    • Author(s)
      Savan, R., Endo, M., Sakai, M.
    • Journal Title

      Molecular Immunology 41

      Pages: 531-539

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A sensitive and rapid detection of white spot virus by loop-mediated isothermal amplification (LAMP) method2004

    • Author(s)
      Kono, T., Savan, R., Sakai, M., Itami, T.
    • Journal Title

      Journal of Virological Methods 115

      Pages: 59-65

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A sensitive and rapid detection of eswardsiellosis by loop-mediated isothermal amplification (LAMP) method.2004

    • Author(s)
      Savan, R., Igarashi, A., Matsuoka, S., Sakai, M
    • Journal Title

      Applied and Environmental Microbiology 70

      Pages: 621-624

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2024-03-26  

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