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卵丘細胞から分泌されるプロジェステロンの減数分裂再開能に関する研究

Research Project

Project/Area Number 04J05263
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Applied animal science
Research InstitutionHiroshima University
Research Fellow 山下 泰尚  広島大学, 大学院生物圈科学研究科, 特別研究員(DC1)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Keywords減数分裂再開 / 卵丘細胞 / 膨潤 / 性腺刺激ホルモン / EGF-like growth factor / Metalloproteinase / 排卵 / 体外成熟培養 / プロジェステロン / EGF様因子 / プロスタグランディン / p38MAPK / cholesterol生合成経路 / MAS / GVBD
Research Abstract

近年,性腺刺激ホルモンの刺激によりEGF-like growth factor(Amphiregulin; AREG, Epiregulin; EREG)が順粒層細胞および卵丘細胞において発現し,EGF受容体を介してparacrine的あるいはautocrine的に卵丘細胞を刺激し,卵丘細胞の膨潤を誘起させることが報告された.AREG, EREGの分泌には,proteaseによる修飾が必要であるが,排卵過程において,AREGおよびEREGを切断・分泌させるproteaseに関する報告はこれまで全く無い.近年,マウスは胚性幹細胞においてADAM17IがAREGおよびEREGを細胞膜表面から切断させることが報告された.我々は,マウス卵丘細胞のマイクロアレイ解析からADAM17が排卵過程に発現していることを見出したことから,ブタ卵丘細胞卵子複合体(COCs)の体外培養系を用いて,卵丘細胞における詳細な役割を検討した.ブタCOCsをFSH+LH添加培地で培養し,ADAM17発現量と活性値を検出した結果,ADAM17発現は,培養10時間後から有意に増加し,40時間まで維持していた.活性値も同様の傾向を示した.FSH+LH添加培地にADAM17阻害剤(TAPI-2)を添加して培養すると,FSH, LHにより誘起される高い活性は,TAPI-2により完全に抑制され,AREGおよびEREGにより活性化されるERK1/2,卵丘細胞の膨潤が低下された.さらに,ラット順位層細胞(GC)に4dam]7siKNAを導入すると,Adam17siRNAは,FSHにより誘導されるERK1/2のリン酸化を抑制したこと,活性型AREGの添加によりその抑制は回復したことから,ADAM17の発現が,EGF-like growth factorを活性型へと変化させ,EGF受容体を刺激する結果,卵丘細胞の膨潤が誘起されることが明らかとなった.

Report

(3 results)
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report

Research Products

(11 results)

All 2006 2005 2004

All Journal Article (11 results)

  • [Journal Article] Effect of semen collection in extender solution on the characteristics of goat spermatozoa.2006

    • Author(s)
      Yamashiro H
    • Journal Title

      Journal of Reproduction and Development 52・3

      Pages: 397-406

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhanced freezability of goat spermatozoa collected into tubes containing extender supplemented with bovine serum albumin (BSA).2006

    • Author(s)
      Yamashiro H
    • Journal Title

      Journal of Reproduction and Development 52・3

      Pages: 407-414

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of Semen Collection in Extender Solution on Characteristic of Goat Spermatozona.2006

    • Author(s)
      Yamashiro H
    • Journal Title

      Journal of Reproduction and Development 52・3(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle.2006

    • Author(s)
      Wang HF
    • Journal Title

      Reproductive Biology and Endocrinology 3・4(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Enhanced Freezability of Goat Spermatozoa Collected in Tubes Containing Extender Supplemented with Bovine Serum Albmin (BSA).2006

    • Author(s)
      Yamashiro H
    • Journal Title

      Journal of Reproduction and Development 52・3(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Transcripts encoding the enzymes that convert acetyl-CoA to cholesterol are induced in cumulus cells and are essential for progesterone biosynthesis and meiotic resumption of porcine oocvtes.2005

    • Author(s)
      Shimada M
    • Journal Title

      Japanese Journal of Reproductive Endocrinology 10

      Pages: 15-20

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Gonadotropin-induced delta 14-reductase and delta 7-reductase gene expression in cumulus cells during meiotic resumption of porcine oocytes2004

    • Author(s)
      Yamashita Y
    • Journal Title

      Endocrinology 146

      Pages: 186-194

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes2004

    • Author(s)
      Shimada M
    • Journal Title

      J Mol Endocrinol 33

      Pages: 209-225

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Down-regulated expression of A disintegrin and metalloproteinase with thrombospondin-like repeats-1 by progesterone receptor antagonist is associated with impaired expansion of porcine2004

    • Author(s)
      Shimada M
    • Journal Title

      Endocrinology 145

      Pages: 4603-4614

    • Related Report
      2004 Annual Research Report
  • [Journal Article] FSH-induced activation of PI3-kinase-PKB pathway is essential for LH receptor formation in cumulus cells during meiotic resumption of porcine oocytes2004

    • Author(s)
      Shimada M
    • Journal Title

      Jpn J Reprod Endocrinol 9

      Pages: 49-54

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of adding luteinizing hormone to a medium containing follicle stimulating hormone on progesterone-induced differentiation of cumulus cells during meiotic resumption of porcine oocytes.2004

    • Author(s)
      Shimada M
    • Journal Title

      Anim Sci J 75

      Pages: 515-524

    • Related Report
      2004 Annual Research Report

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Published: 2004-03-31   Modified: 2016-04-21  

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