| Project/Area Number |
05041098
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Field Research |
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine (1994)
Kochi Medical School (1993) |
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Principal Investigator |
AGATSUMA Takeshi Obihiro University of Agriculture and Veterinary Medicine, 畜産学部, 助教授 (40117031)
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| Co-Investigator(Kenkyū-buntansha) |
ウォン Z. 湖北省, 住血吸虫防圧研究所, 所長
ブレーラー D. ジェームズ, クック大学・理学部, 講師
ロー C.T. 台湾国立陽明医学院, 医学部, 助教授
イトイ I. マレーシア国立医学研究所, 寄生虫部, 主任研究官
ウパタム S. マヒドール大学, 理学部, 教授
SUGIYAMA Hiromu National Institute of Health, 寄生動物部, 主任研究官 (00145822)
TAGUCHI Takahiro Kochi Medical School, 医学部, 助手 (80127943)
HIRAI Hirohisa Kyoto University, 霊長類研究所, 助手 (10128308)
KAWANAKA Masanori National Institute of Health, 寄生動物部, 室長 (50109964)
HABE Shigehisa Fukuoka University, 医学部, 講師 (70037430)
HIRATA Mizuki Kurume University, 医学部, 助教授 (70080629)
BLAIR David James Cook University
LO Chin-tson National Yang-Ming Medical College
ITHOI Init Institute for Medical Research
UPATHAM Suchart Mahidol University
WANG Zaihua Hubei Institute of Schistosomiasis Control
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 1994: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1993: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | Phylogeny / Schistosoma japonicum / S.mekongi / S.malaynsis / Mitochondrial DNA / PCR / C-bannding pattern / Chiasma / Karyotype |
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| Research Abstract |
Phylogeny among Asian Schistosoma flukes have not been known so far. In the present study, PCR technique was used to make up a molecular phylogeny on three Asian Schistosoma species, S.japonicum, S.mekongi, and S.malaynsis. A region of cytochrome C oxidase subunit I (COI) was amplified, and the fragment was cloned into TA-vector. The ABI sequencer/the Dye primer method were used. In total, 395 base pairs were sequenced, and analyzed with Paup (Maximum Parsimony Method) and Clustal V (The Neighbor-Joining Method). As outgroups, two African species, S.mansoni and S.haematobium were used. Both of the two methods constructed the same phylogenetic trees. That is, S.mekongi and S.malaynsis was clustered first, and joined with S.japonicum. The two African species were joined into a cluster, and bound with the Asian species.
C-banding pattern analysis of the chromosomes was also performed The pattern showed closer relationships between S.mekongi and S.malaynsis, supporting the molecular phylogenetic tree obtained in this study. Karyotypic analysis of the snail host species, Robertsiella gismani, Neotricula aperta, and Oncomelania nosophora were carried out. It was found that chromosome numbers were all 2n=34, but sex chromosomes seem to be of XY type and XO type for Robertsiella gismani and Neotricula aperta, respectively. In the case of Oncomelania nosophora, no sex chromosome differenciation occurred.
Infection experiments were also carried out. Three Japanese snails, Oncomelania nosophora, O.minima and Bythinella nipponica were used. It was found that only Oncomelania nosophora was susceptible to S.japonicum and S.malaynsis infection.
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