Study on hepatitis C virus infection in Indonesia
Grant-in-Aid for international Scientific Research
|Allocation Type||Single-year Grants|
|Research Institution||Kobe University|
HOTTA Haku Kobe University School of Medicine, 医学部, 助教授 (40116249)
KATAYAMA Yuko Kobe University School of Medicine, 医学部, 助手 (10224461)
HOMMA Morio Kobe University School of Medicine, 医学部, 教授 (10004566)
|Project Period (FY)
Completed(Fiscal Year 1993)
|Budget Amount *help
¥4,500,000 (Direct Cost : ¥4,500,000)
Fiscal Year 1993 : ¥4,500,000 (Direct Cost : ¥4,500,000)
|Keywords||Hepatitis C virus / Subtype / Chronic liver disease / Anti-HCV antibody / Indonesia|
1. Epidemiological study on hepatitis C virus (HCV) in Indonesia.
Anti-HCV was detected in 49%(27/55) of patients with chronic hepatitis, 63%(38/60) with liver cirrhosis and 41%(12/29) with hepatoma by using second generation ELISA. Thus, HCV is a major etiologic agent of chronic liver diseases in Indonesia.
Prevalence of anti-HCV among doctors in a university hospital was 3.4% (5/149), which was almost the same as that of the normal adult population in Indonesia. On the other hand, anti-HCV was detected in 15%(3/20) of nurses and 5.9%(5/85) of midwives in the same hospital. These results suggest that the latter two are high-risk groups for HCV infection.
2. Virological study on HCV in Indonesia.
(1)Identification of novel subtypes of HCV.
The core, El and NS5 regions of the HCV genome were amplified from anti-HCV-positive sera by RT-PCR. Based on the sequences of the amplified fragments and the reported subtypes, phylogenetic analysis was performed to determine subtypes of the Indonesian HCV. The results demonstrate the presence of novel subtypes of HCV ; HCV-1d, which was found to prevail in Indonesia, and HCV-3c with much less prevalence.
(2)Prevalence of each subtype among HCV in Indonesia.
45%(9/20) of HCV isolates from chronic hepatitis patients were HCV-1b, and 20%(4/20) were HCV-1d. Among those from liver cirrhosis patients, 34% (11/32) were HCV-1b and another 34% was HCV-1d. Prevalence of HCV-1d appeared to be higher in patients with liver cirrhosis than chronic hepatitis, suggesting that HCV-1d may be more virulent than other subtypes.
(3)Analysis of antigenicity of the core protein of HCV.
An HCV variant (Td-6) was obtained, the core protein of which might antigenically differ from ordinary HCV isolates.
Research Products (6results)