Grant-in-Aid for international Scientific Research
|Allocation Type||Single-year Grants|
|Research Institution||Kyoto Institute of Technology|
TANAKA Nobuo Kyoto Institute of Technology, 繊維学部, 教授 (60127165)
BARNHART Elizabeth Centers for Disease Control, 防疫センター(米国), 研究員
PATTERSON Donald g. Centers for Disease Control, 防疫センター(米国), 室長
SVEC F. チェコ国立科学アカデミー, 高分子化学研究所, 室長
FRECHET J.M. コーネル大学, 化学教室(米国), 教授
YOKOYAMA Kazunari National Institute of Agro-environmental Sciences, 農業環境技術研究所, 研究員
HOSOYA Ken Kyoto Institute of Technology, 繊維学部, 助手 (00209248)
FRECHET Jean m.j. Cornell University
SVEC Frantisek Czech Academy of Science
|Project Period (FY)
Completed(Fiscal Year 1993)
|Budget Amount *help
¥3,000,000 (Direct Cost : ¥3,000,000)
Fiscal Year 1993 : ¥3,000,000 (Direct Cost : ¥3,000,000)
|Keywords||Polymer-based separation media / Isolation of DNA related compounds / High performance liquid chromatography|
In our joint research project "Development of new separation media for isolation of DNAs", under the Monbusho International Scientific Research Program (No. 05044054), the following four subjects have been investigated.
(1) Development of high performance polymer-based separation media. To improve relatively low column efficiency and poor mechanical strength of the column packed with size poly-disperse polymer beads prepared by traditional suspension method, size mono-disperse beads were prepared by two-step swelling and polymerization method. These size mono-disperse beads showed excellent performance and the pore structure was found to be controllable utilizing both of porogenic solvent and seed polymer.
(2) Development of hydrophilic polymer-based packing material. The two-step swelling and polymerization method could afford size-monodisperse poly(glycerol monomethacrylate-co-glycerol dimethacrylate) beads. These beads were enough hydrophilic to prevent retention with polypeptide, while retained hydrophobic small molecules. These characteristics were suitable for purification of hydrophilic large molecules from small molecules.
(3) Development of new rod type separation media. Polymer-based continuous rod type separation media could be prepared in inert PEEK columns. These rod columns involved huge flow-through pores and micropores which was suitable characteristics for isolation of biomolecules. Silica-based rod columns could be also prepared and utilized in reversed-phase mode.
(4) Development of concentration method and treatment of organohalides. Restricted-access separation media were found to be utilized in isolation of toxic poly-chlorinated biphenyl from human serum. These separation media could also concentrate organohalides such as dioxins from aqueous environment which could be decomposed by uv irradiation or sunlight.