Studies on the molecular mechanism of heat shock protein 47.
Grant-in-Aid for Overseas Scientific Survey.
|Allocation Type||Single-year Grants|
|Research Institution||Kyoto University|
NAKAI Akira Kyoto University, Chest Disease Research Institute, Instractor, 胸部疾患研究所, 助手 (60252516)
EL-THAHER Talal Imperial College, Dept. of Chemistry, Research F
MILLER Andrew Imperial College, Dept. of Chemistry, Lecturerer
HIRAYOSHI Kazunori Kyoto University, Chest Disease Research Institute, 胸部疾患研究所, 助手 (80199108)
NAGATA Kazuhiro Kyoto University, Chest Disease Research Institute, 胸部疾患研究所, 教授 (50127114)
|Project Period (FY)
Completed(Fiscal Year 1993)
|Keywords||Heat Shock Protein / Stress Protein / Molecular Chaperone / Structual Analysis / Collagen / Molecular Biology / Intracellnlar transport / Intermolecular Association|
Heat shock protein 47 (HSP47) can bind to collagen and its expression is correlated with that of collagen in many cell lines and tissues. We think that HSP47 has a significant role for procollagen to be secreted correctly. HSP47 exist in the ER and associates transciently with a newly synthesized procollagen. Proteins that facilitate the folding and assembly of polypeptides are called as molecular chaperone. We think HSP47 acts as a molecular chaperone by a substrate-specific manner. Interestingly, the binding of HSP47 with collagen is regulated by a physiological change of pH. In this project, our aims are to reveal the molecular structure of HSP47 and to identify the binding site in collagen.
The progresses of collaboration in this year are listed.
1) The researchers in Japan made the expression vector of HSP47 and sent it to researchers in U.K. They established the purification protocols of HSP47 and now they can get a highly purified HSP47.
2) The researchers in U.K. started the structural analysis of HSP47 by CD spectrum.
3) The crystal structures of some serpin family members are known. Because HSP47 belongs to the serpin family, its structure can be speculated by computer analysis.
Research Products (17results)