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Principle of Stress Response

Research Project

Project/Area Number05268102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionTokyo Metropolitan Organization for Medical Research

Principal Investigator

YAHARA Ichiro  The Tokyo Metropolitan Institute of Medical Science, Vice-Director, 副所長 (60109957)

Co-Investigator(Kenkyū-buntansha) SEKIMIZU Kazuhisa  Kyushu Univ., Fac.Pharmaceutical Sci., Professor, 薬学部, 教授 (90126095)
NAKAMURA Yoshikazu  Univ.Tokyo, Inst.Med.Res., Associate Professor, 医科学研究所, 助教授 (40114590)
SHINAGAWA Hideo  Osaka Univ., Res.Inst.Microbial Diseases., Professor, 微生物病研究所, 教授 (40029799)
ITO Koreaki  Kyoto Univ., Inst.Virus Res., Professor, ウイルス研究所, 教授 (90027334)
ISHIHAMA Akira  Nat.Inst.Genetics, Dept.Mol.Genetics, Professor, 分子遺伝, 教授 (80019869)
Project Period (FY) 1993 – 1995
Project Status Completed(Fiscal Year 1996)
Budget Amount *help
¥278,000,000 (Direct Cost : ¥278,000,000)
Fiscal Year 1995 : ¥94,000,000 (Direct Cost : ¥94,000,000)
Fiscal Year 1994 : ¥94,000,000 (Direct Cost : ¥94,000,000)
Fiscal Year 1993 : ¥90,000,000 (Direct Cost : ¥90,000,000)
Keywordsstress response / stress protein / heat shock protein / molecular chaperone / oxidative stress / SOS response / transcription factor / proteasome / 細胞骨格 / 転写制御 / 蛋白質の分泌 / タンパク質の分泌
Research Abstract

1) Molecular chaperone function of HSP90, a major stress protein, has been clarified..
2) Molecular structure of RuvC,an endonuclease induced by the SOS response which recognizes intermediate structures of DNA recombination has been determined by X-ray crystallography. Function of RuvC was also anlyzed.
3) Subunit proteins of 26S and 20S proteasomes have been identified. Their cDNA's have been isolated and their functions have been extensively analyzed.
4) sigma32, a stress-specific transcription factor, has been shown to be quantitatively regulated by stimulation of translation and its stabilization.
5.A new protease, FtsH,has been discovered and shown to belong to the AAA family. In addition, FtsH has been shown to degradate sigma32.
6.Intermediate structures of substrate proteins recognized by chaperonin have been investigated.
7.Relaxation mechanism of supercoil structure of plasmid DNA upon heat shock has been investigated.
8.It was found that intranuclear ongogenic product Myb interacts with the heat shock factor HSF3.
9.Signal transduction mechanism involved in the activation of NF-kB upon stress response has been elucidated.
10.Processing and cell surface expression of Hsp65 in macrophage has been investigated upon paracyte infection.

Report

(4results)
  • 1996 Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • 1993 Annual Research Report

Research Products

(37results)

All Other

All Publications

  • [Publications] Yonehara,M.et al.: "Heat-induced chaperone activity of HSP90." J.Biol.Chem.271. 2641-2645 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura,Y.et al.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Science. 268. 1362-1365 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Miyata,Y.And Yahara,I.: "Interaction between casein kinase II and the 90-kDa stress protein,HSP90." Biochemistry. 34. 8123-8129 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Higashitani,N.et al.: "SOS induction in Escherichia coli by single-stranded DNA of mutant filamentous phage:Monitoring by cleavage of LexA repressor." J.Bacteriol.177. 3610-3612 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimoike,T.et al.: "Product of a new gene,syd,functionally interacts with SecY when overproduced in Escherichia coli." J.Biol.Chem.270. 5519-5526 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasukawa,T.et al.: "Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin." J.Biol.Chem.270. 25328-25331 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinagawa, H.: "Processing the Holliday junction in homologous recombination." TIBS. 21. 107-111 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatanaka, H.: "Tertiary structure of destrin and structural similarity between two actin-regulating protein families." Cell.85. 1047-1055 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, Y.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Science. 268. 1362-1365 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomoyasu, T.: "Escherichia coli FtsH is a membrane-bound, ATP-dependent protease which degrades the heat-shock transcription factor sigma^<32>." EMBO J.14. 2551-2560 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, Y.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Biochemistry. 34. 8123-8129 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jeon, Y.H.: "Solution structure of the C-terminal domain of RNA polymerase alpha subunit responsible for the contact with trans-acting transcription factors and cis-acting UP element of promoter." Seience. 270. 1945-1997 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kihara, A.: "FtsH is required for proteolytic elimination of uncomplexed forms of Sec Y,an essential protein translocase subunit." Proc.Natl.Acad.Sci.USA. 92. 4532-4536 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Saito, A.: "Identification of four acidic aminoacids that constitute the catalytic center of the RuvC Holliday junction resolvase." Proc.Natl.Acad.Sci.USA. 92. 7470-7474 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kominami, K.: "Nin1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae." EMBO J.14. 3105-3115 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotoh, Y.: "Involvment of the MAP kinase cascade in Xenopus mesoderm induction." EMBO J.14. 2491-2498 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimoike, T.: "Product of a new gene, syd, functionally interacts with SecY when overproduced in Esherichia coli." J.Biol.Chem.270. 5519-5526 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogata, K.: "Solution structures of the c-Myb DNA-binding domain : comparison between the free and DNA-complexrd forms." Nature Structural Biology. 2. 309-320 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue, A.: "70kDa heat shok cognate protein interacts directly with the N-terminal region of the retinoblastoma gene product pRb-Identification of a novel region of pRb-mediating protein interaction." J.Biol.Chem.270. 22571-22576 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yonehara, M. et al.: "Heat-induced chaperone activity of HSP90." J. Biol. Chem.271. 2641-2645 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimura, Y. et al.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Science. 268. 1362-1365 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Miyata, Y. And Yahara, I.: "Interaction between casein kinase II and the 90-kDa stress protein, HSP90." Biochemistry. 34. 8123-8129 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Higashitani, N. et al.: "SOS induction in Escherichia coli by single-stranded DNA of mutant filamentous phage : Monitoring by cleavage of LexA repressor." J. Bacteriol.177. 3610-3612 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimoike, T. et al.: "Product of a new gene, syd, functionally interacts with SecY when overproduced in Escherichia coli." J. Biol. Chem.270. 5519-5526 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasukawa, T. et al.: "Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin." J. Biol. Chem.270. 25328-25331 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Minami,Y.et al.: "The carboxy-terminal region of mammalian HSP90 is required for its dimerization and function in vivo." Mol.Cell.Biol.14. 1459-1464 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kanemori,M.et al.: "Effects of reduced levels of GroE chaperons on protein metabolism:Enhanced synthesis of heat shock proteins during steady-state growth of Escherichia coil." J.Bacteriol.176. 4235-4242 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Higashitani,A.et al.: "Multiple DNA conformational changes induced by an initiator protein precede the nicking reaction in a rolling cirlce replication origin." J.Mol.Biol.237. 388-400 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ito,K.et al.: "Regulation of lysyl-tRNA synthetase espression by histone-like protein H-NS of Escherichia coli." J.Bacteriol. 176. 73-83 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ariyoshi,M.et al.: "Atomic structure of the RuvC resolvase:A Holliday junction-specific endonuclease from E.coli." Cell. 78. 1063-1072 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ogata,Y.et al.: "Identification of DNA topoisomerases involved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli." Mol.Gen.Genet.244. 451-455 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Iida,K.: "Isolation of a yeast essential gene,COF1,that encodes a homologue of mammalian cofilin,a low-Mr actin-binding and depolymerizing protein." Gene. 124. 115-120 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanikawa,J.: "Recognition of specific DNA sequences by the c-myb proto-oncogene product:role of three repeat units in the DNA-binding domain." Proc.Natl.Acad.Sci.USA. 90. 9320-9324 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akiyama,Y.: "Folding and assembly of bacterial alkaline phosphatase in vitro and in vivo" J.Biol.Chem.268. 8146-8150 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shiba,T.: "Escherichia coli RuvA and RuvB proteins involved in recombination repair:Physical properties and interactions with DNA." Mol.Gen.Genet.237. 395-399 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yuzawa,H.: "Heat induction of sigma-32 synthsis mediated by mRNA secondary structure:a primary step of the heat shock responsein Escherichia coli." Nucleic Acid Res.21. 5449-5455 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamagishi,M.: "Regulation of the Escherichia coli rmf gene encoding ribosome modulation factor (RMF):Growth phase-and growth rate-dependent control." EMBO J.12. 625-630 (1993)

    • Related Report
      1993 Annual Research Report

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Published : 1993-04-01   Modified : 2016-04-21  

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