Grant-in-Aid for Scientific Research on Priority Areas.
|Research Institution||KYUSHU UNIVERSITY|
NISHIMOTO Takeharu Kyushu University Graduate School of Medical Science, Professor, 大学院・医学系研究科, 教授 (10037426)
OKAZAKI Tsuneko School of Science, Nagoya University, Professor, 大学院・理学系研究科, 教授 (10022584)
登田 隆 京都大学, 理学部, 講師 (50197894)
柳田 充弘 京都大学, 大学院理学研究科, 教授 (80025428)
関口 睦夫 九州大学, 生体防御医学研究所, 教授 (00037342)
IKAWA Yoji Tokyo Medical and Dental University Medical Research Division, Professor, 大学院・医学系研究科, 教授 (40085618)
KATSURA Isao DNA Research Center, National Institute of Genetics, Professor, 遺伝情報研究センター, 教授 (00107690)
IKEDA Joei School of Medical Science Tokai University, Professor, 総合医学研究所, 教授 (50266467)
TOUE Akio Graduate School of Science University of Tokyo, Professor (90029249)
|Project Fiscal Year
1993 – 1995
Completed(Fiscal Year 1996)
|Budget Amount *help
¥91,900,000 (Direct Cost : ¥91,900,000)
Fiscal Year 1996 : ¥3,000,000 (Direct Cost : ¥3,000,000)
Fiscal Year 1995 : ¥23,000,000 (Direct Cost : ¥23,000,000)
Fiscal Year 1994 : ¥34,600,000 (Direct Cost : ¥34,600,000)
Fiscal Year 1993 : ¥31,300,000 (Direct Cost : ¥31,300,000)
|Keywords||G0 / G1 / DNA replication / Mitosis / Map Kinase / Cycline / cdc2 / Ubiquitin / G protein / DNA複製 / M期 / MAPキナーゼ / サイクリン / ユビキチン / Gタンパク / コビキチン / 細胞周期 / 染色体分配 / G2 / M / ライセンス因子 / プロテアソーム|
MAP kinase cascade in the signal transduction pathway : Map kinase has been identified as a kinase which induce phosphorylation to activate maturation prompting factor (MAF) consisting of cdc2 and cyclin B.Drs.Nishida (Kyoto U.) and Sakata (Kyushu U.) clarified that Mos is a key regulator in the MAP kinase cascade.
Identification of Licensing factor for DNA replication in S phase : In eukaryote, it is very important that the total genomic DNA is replicated only once in the S phase. To do that it has been assumed that cells possess a factor to license only the signal round of DNA replication in S phase. In this research program, Dr.Takisawa identified the protein factor which allow a single round of DNA replication. It is turned out to be a mammalian homologue of S.cereviae Mcm families.
Cyclins in the cell cycle : Cyclins comprise either MPF or SPF with cdc2 or cdks that are so called cell cycle engines. In this context, it is a very important question why cyclins are essential for MPF a
nd SPF functions. Drs Kobayashi (Kyushu U.) and Kishimoto (Tokyou Engineering U.) investigated the interaction between cdc2 and cyclin B during G2/M transition.
Ubiqutin system in the cell cycle : Degradation of proteins depend on either ubiqutin or nonubiqutin cycle.in the cell cycle, the ubiqutin dependent protein degradation is important for the progression of cell cycle. It plays a impartant role for the regulation of cell cycle progression either. Dr.Tanaka (Tokushima U.) has identified proteins comprising the ubiqutin dependent proteasome and cloned the cDNA encoding these proteins.
G protesin in the cell cycle : A small G protein functions as a biological switch. Thus far, many G proteins have been found to be involved in the signal transduction and the protein secretion. RCC1 which was cloned by the group of Dr.Nishimoto (Kyushu U.) functions as a GTP/GDP exchanging factor for a small nuclear GTPase Ran.
Since loss of RCC1 induces premature initiation of mitosis, RCC1 is assumed to be involved in the cell cycle regulation Less