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糖鎖及び糖鎖複合体の解析・分子設計及び再構成

Research Project

Project/Area Number 05274102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo

Principal Investigator

小川 智也  東京大学, 大学院農学生命科学研究科, 教授 (30087572)

Co-Investigator(Kenkyū-buntansha) 遠藤 正彦  弘前大学, 医学部, 教授 (20004616)
隅田 泰生  大阪大学, 理学部, 講師 (70179282)
畑中 保丸  富山医科薬科大学, 和漢薬研, 助教授 (30111181)
木曽 真  岐阜大学, 農学部, 教授 (90092931)
稲垣 冬彦  東京臨床医学総合研究所, 生理活性物質研究部門, 部長 (70011757)
長谷川 明  岐阜大学, 農学部, 教授 (10026429)
梶本 哲也  理化学研究所, 国際フロンティア・糖情報工学, 副チームリーダー (80185777)
楠本 正一  大阪大学, 理学部, 教授 (30028253)
Project Period (FY) 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥135,400,000 (Direct Cost: ¥135,400,000)
Fiscal Year 1996: ¥37,300,000 (Direct Cost: ¥37,300,000)
Fiscal Year 1995: ¥36,000,000 (Direct Cost: ¥36,000,000)
Fiscal Year 1994: ¥33,100,000 (Direct Cost: ¥33,100,000)
Fiscal Year 1993: ¥29,000,000 (Direct Cost: ¥29,000,000)
KeywordsaFGF / ヘパリン / セレクチン / LTA / ビオチン化プローブ / ヒアルロニダーゼ / LIF / GAG / シアロ複合糖質 / リポタイコ酸 / 光アフィニティープローブ / ピューロマイシン / コンドロイチン硫酸 / ユビキチン / リピドA / リガンド / グアニジドガラクトース / セラミド / プロテオグリカン
Research Abstract

稲垣はaFGFとヘパリンの相互作用、ミッドカインによるヘパリン認識および、ヒアルロン産結合ドメイン1inkの立体構造決定に関し、NMR法により成果を得た。隅田は、グラム陽性細菌のLTA画分からIL-6誘導活性を示す成分を分離し、それらが高分子量の糖脂質であることを解明した。長谷川と木曽は、種種のガングリオシドの合成に成功した。畑中は、新規プローブの開発とラベルタンパク質の単離操作の効率化に成功した。遠藤は、天然型および非天然型のグリコサミノグリカンの、酵素的合成ルートを開拓した。小川はコンドロイチン硫酸の再構成実験を通して、その生合成系の解析を共同研究により追求し、成果を得た。またLIFを対象として、動的細胞発現系を利用して糖鎖機能の解析を行った。北原は、糖タンパク質輸送阻害剤の機能ドメインに関して合成化学的研究を行い、成果を得た。豊島は非天然型の糖脂質を調製して、生理活性相関に知見をえた。長谷は、脳特異的糖鎖を調製し、人工糖脂質としてプローブ化した。岡崎はセラミドシグナル系の解析を進め、そのリン酸化誘導体の機能解析に知見を得た。以上の研究実績は、糖鎖複合体の解析・分子設計および再構成の総合的観点から、国際的に先端を行くものである。

Report

(4 results)
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Takagaki,K.et al.: "Characterization and bioligocal significance of sialy alpha-2-3 galactosyl beta 1-4 xylosylbeta 1-(4-methvlumbelliferone)synthesized in cultured human skin fibroblasts." J.Biochem.119. 697-702 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hatanaka,H.,et al.: "Tertiary structure of Destrin and structural similarity between two actin-regulating protein families" Cell. 85. 1047-1055 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ehara,T.et al.: "STotal synthesis of VIM-2 ganglioside isolated from human chronic myelogenous leukemia cells." Carbohydr.Res.281. 237-252 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ozaki,Y.,et al.: "Preparation of neoglycolipids with a definite suger chain moiety from glycoproteins." Anal.Biochem.241. 151-155 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sawai,H.,et al.: "Ceramide-induced translocation of protein-kinase c-delta and c-epsilon to the cytosol-implication in apoptosis." J.Biol.Chem.272(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Uozumi,N.et al.: "A fluorescent assay-method for GDP-L-Fuc-N-Acetyl-beta-D-glucosaminide alpha-1-6Fucosyltransferase activity,Involving high-performance liquid-chromatography." J Biochem.120. 385-392 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kirsch,P.ら: "Synthesis of N-Acetylgluco saminyl Aspargine-Substitnted Puronycin Analogues" Bio. Med. chem.3. 1631-1636 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichikawa,S.ら: "Identification of the Receptor-recognition Surface of Boubyxin-II, an Insulinlike peptide of the Silkmoth Bombyx mori Critical Importance of B-chain Cerdral Part" J. Mol. Biol.253(5). 759-770 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hotta,K.ら: "First total・synthesis of a cholinergic reuron-shecific ganglioside GQ1bα" J.Carbohydr. chem.14. 491-506 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hatanaka,Y.ら: "A Carbene-Generating Biotihylated Lactosylceramide Analog as Novel Photoreactive Substrate for GM3 Synthase" Bio Med.Chem. Lett.5. 2859-2864 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suda,S.ら: "Cytokine-Inducing Glyco lipids in the Lipoteichoic Acid Fracrion from Enterococcus hirae" FEMS Immun Med Microbiol.12. 97-112 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Saitoh,H.ら: "Enzymic reconstitution of glycosaminoglycan oligosaccharide chain using the transglycosylation reaction of bovine testicular hyaluronidase" J.Biol.Chem.270. 3741-3747

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Hatanaka et al.: "Tertiary structuve of erabutoxin b in aqueous solution as elucidated by two-dimensional nuclear magnetic resonance" J.Mol.Biol.240. 115-166 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Nagata et al.: "Solution structurve of the epidermal growth factor like domain of heregulin-α.a ligand for p180^<2rbB-4>" The EMBO J.13. 3517-3523 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] C.-T.Yuen K.et al.: "Sulfated Blood Group Lewis^a - A superior oligosaccharide ligand for human E-selectin" J.Biol.Chem.269. 1595-1598 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H-K,Ishida et al.: "Total synthesis of ganglioside GQlb" Carbohydr.Res.260. c1-c6 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Hatanaka et al.: "A Novel Family of Aromatic Diazirines for Photoaffinity Labeling" J.Org.Chem.59. 383-387 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Hatanaka et al.: "Synthesis of Nitro-Substituted Aryl Diazirines.An Entry to Chromogenic Carbene Precursors for Photoaffinity Labeling" Chem.Pharm.Bull.42. 826-831 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fumitaka Goto et al.: "Recent aspects of glycoconjugate synthesis:A synthetic approach to the linkage region of proteoglycans" Pure & Appl.Chem.65. 793-801 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yuji Matsuzaki et al.: "Stereocontrolled synthesis of GD_2" Carbohydr,Res.242. C1-C6 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akira Hasegawa et al.: "Total synthesis of ganglioside GMl and GDla" J.Carbohydr.Chem.12. 354-361 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasuo Suda et al.: "The synthesis and in uitro and in vivo stability of 5-fluorouracil prodrugs which possess serum albumin binding potency" Biol.Pharm.Bull.16. 876-878 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akira Takeda et al.: "Contribution of carbohydrate antigen sialyl lewis A and sialyl lewis X to adhesion of human cancer cells to vascular endothelium." Cancer Res.53. 354-361 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshikazu Takaoka et al.: "Inhibition of N-acetylglucosaminyl transferase enzymes:chemical-enzymatic synthesis of new five-membered acitamido azasugars" J.Org.Chem.58. 4809-4812 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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