Grant-in-Aid for Co-operative Research (A).
Morphological basic dentistry
|Research Institution||Showa University|
YOSHIKI Shusaku Showa University, School of Dentistry, Professor, 歯学部, 教授 (30085740)
小澤 英浩 新潟大学, 歯学部, 教授 (60018413)
久米川 正好 明海大学, 歯学部, 教授 (40049367)
須田 立雄 昭和大学, 歯学部, 教授 (90014034)
鈴木 不二男 大阪大学, 歯学部, 教授 (40028717)
佐々木 哲 東京医科歯科大学, 歯学部, 教授 (80013803)
SUDA Tatsuo Showa Univ., School of Dentistry, Professor
KUMEGAWA Masayoshi Meikai Univ., School of Dentistry, Professor
SASAKI Satoshi Tokyo Medical and Dental Univ., School of Dentistry, Professor
SUZUKI Fujio Osaka Univ., School of Dentistry, Professor
OZAWA Hidehiro Niigata Univ., School of dentistry, Professor
|Project Fiscal Year
1993 – 1994
Completed(Fiscal Year 1994)
|Budget Amount *help
¥11,500,000 (Direct Cost : ¥11,500,000)
Fiscal Year 1994 : ¥5,300,000 (Direct Cost : ¥5,300,000)
Fiscal Year 1993 : ¥6,200,000 (Direct Cost : ¥6,200,000)
|Keywords||bone formation / osteoblast / chondrocyte / osteoclast / osteocyte / BMP / in situ hybridization / cell culture / 骨芽細胞 / 軟骨細胞 / 破骨細胞 / 骨細胞 / 培養細胞 / In sltu hrbridization / レーザー顕微鏡 / In situ hybridization|
The mechanism involved in bone formation has been investigated using morphological, biochemical and molecular biological techniques, and the following results are obtained.
1.The important roles of BMP-2 in the differentiation process of osteoblasts has been demonstrated using various kinds of cell lines (Yoshiki S., et al.).
2.The age-related decreases in mRNA expression for bone matrix proteins in osteoblasts were revealed by in situ hybridization(Yoshiki S., et al.).
3.Several genes for BMPs and BMP receptors were cloned. The changes in mRNA expression for Hox genes were analyzed during the process of ectopic bone formation induced by BMP(Sasaki S., et al.).
4.BMP-2 inhibited myogenic differentiation in myoblasts, and converted their differentiation pathway into osteoblast cell lineage (Suda T., et al.).
5.The tissue distribution of ChM-1 was revealed by immunohistochemistry and in situ hybridization. The important roles of ChM-2 was demonstrated in the process of bone formation and bone resorption(Suzuki F., et al.).
6.The new technique for isolation of osteocytes was developed (Kumegawa M., et al.).
7.Interaction among osteoblasts, osteocytes and osteoclasts were investigated using ultrastructural histochemistry and radar microscope(Ozawa H., et al).