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Development of Efficient Recycling Utilization Method of Phosphorus Resources by Using Acid Phosphatase-Secreting Function of Plant Roots

Research Project

Project/Area Number 05453155
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TADANO Toshiaki  Hokkaido Univ., Fac.of Agriculture, Pro., 農学部, 教授 (40001440)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsAcid phosphatase / Secretion / lupin / rhizosphere / Organic phosphate compounds / Inorganic difficultly soluble phosphate compounds / Recycling use of phosphorus / Phosphirus resources / 分泌性酸性フォスファターゼ / 酸性フォスファターゼの分泌部位 / 分泌性酸性フォスファターゼの合成部位 / 燐欠乏 / 分泌性酸性フォスファターゼの種間差異 / 低燐耐性 / ルーピン / 分泌性酸性フォスファターゼの性質 / 燐資源の効率的循環利用法 / 土壌の有機態燐の分解・利用
Research Abstract

(1) Acid phosphatase activities in the rhizosphere and bulk soils of sixteen plants grown in the agricultural fields and wild lands were investigated. The activity in the rhizosphere was 1.1-26.8 times higher than that in bulk soils. (2) In the soil culture experiments, it was assumed that acid phosphatase secreted from roots of lupin, tomato and azuki been, hydrolized organic phosphate compounds and released inorganic phosphate, thus increased absorption of phophorus by these plants. (3) Acid phosphatase secreted from lupin roots hydrolyzed organic phosphate compounds containing in the maize leaves and city sludges and released inorganic phosphate. (4) Phosphorus absorption of sugar beet and tomato, which are low in low phosphorus soil tolerance, grown in the soil culture condition was increased and the growth was improved by adding crude secreted acid phosphatase solution, obtained from lupin, to the rhizosphere of these plants. (5) It was clarified that acid phosphatase was secreted … More from all parts of root system in lupin. The enzyme was assumed to be synthesized mainly in the epidermis cells and secreted to the rhizosphere efficiently. the synthesis and secretion were enhanced when the plants sufferred from phosphorus deficiency lightly. (6) Characteristics of acid phosphatase secreted from roots of lupin and tomato were not quite different, but amount of the enzyme secreted was far lager in lupin than in tomato. (7) The acid phosphatase secreted from lupin roots was distributed within 2.5 mm from the root surface. Depletion of organic phosphate compounds in the rhizosphere was well corresponded to the distribution pattern of acid phosphatase in the rhizosphere. (8) It was shown that the distribution pattern of organic acids such as citric acid, malic acid and oxalic acid was similar to that of acid phosphatase. These organic acids released phosphate from difficultly soluble inorganic phosphate compounds contained in soils. (9) From these experimental results, it was concluded that acid phosphatase-secreting function of plant roots is able to be used for the development of efficient recycling utilization of phosphorus resources, of which deposited amount in the world is limited. Less

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] T.Tadano, K.Komatsu:"Utilizasion of Orgamic Phosphorus in Soil by Plant Roots." 15th World Congress of Soil Sclence.,Int.Soc.Soil Sct.Vol.9. 521-522 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Tadano:"Diagnosis and Nutrient Management for Ctops.Int.Seminar Proceedings on Soil Science and Agricultural Technology in the Philippines." Soil Research and Deveiopment Center,Quezon,Philippines.89-95 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Ozawa, M.Osaki, H.Matsui.M.Honma, T.Tadano:"Purifilcation and Properties of Acid Phosphatase Secreted from Lupin Roots under Phosphorus-Deficiency Conditions." Soil Sci.Plant Nutr.,. 41. 461-469 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Li.and T.Tadano: "Comparison of Characteristics of Acid Phosphatases Secreted from Roots of Lupin and Tomato." Soil Sci.Plant Nutr.42. 753-763 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Li.M.Osaki, M.Homma and T.Tadano:"Purification and Characterization of Phytase Induced in Tomato Roots under Phosphorus-Deficient Conditions." Soil Sci.Plant Nutr.,. 43. 179-190 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Li, T.Shinano and T.Tadano: "Distribution of Exudates of Lupin Roots in the Rhizosphere under Phosphorus-Deficient Conditions." Soil Sci.Plant Nutr.,. 43. 237-245 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Tadano, K.Komatsu: "Utilizasion of Orgamic Phosphorus in Soil by Plant Roots." 15th World Congress of Soil Science., Int.Soc.Soil Sci. vol.9. 521-522 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Tadano: "Diagnosis and Nutrient Management for Crops." Proceedings on Soil Science and Agricultural Technology in the Philippines. Soil Research and Development Center, Quezon, Philippines.89-95 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Ozawa, M.Osaki, H.Matsui, M.Honma, T.Tadano: "Purification and Properties of Acid/Phosphatase Secreted from Lupin Roots/under Phosphorus-Deficiency Conditions." Soil Sci.Plant Nutr.41 (3). 461-469 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Li.and T.Tadano: "Comparison of Characteristics of Acid Phosphatases Secreted from Roots of Lupin and Tomato." Soil Sci.Plant Nutr.42 (4). 753-763

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Li.M.Osaki, M.Homma and T.Tadano: "Purification and Characterization/of Phytase Induced in Tomato Roots under Phosphorus-Deficient Conditions." Soil Sci.Plant Nutr.43 (1). 179-190

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Li, T.Shinano and T.Tadano: "Distribution of Exudates of Lupin Roots in the Rhizosphere under Phosphorus-Deficient Conditions." Soil Sci.Plant Nutr.43 (1). 237-245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tadano,T: "Secretion of acid phosphatase by the roots of crop plants under phosphorusdeficient conditions and some properties of the enzyme secreted by lupin roots." Plant and Soil. 155/156. 95-98 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shinano,T: "^<14>C-Allocation of ^<14>C-compounds introduced to a leaf to carbon and nitrogen components in rice and soybean during ripening." Soil Science and Plant Nutrition. 40. 199-209 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shinano,T: "Comparison of root growth and nitrogen absorbing ability between Gramineae and Leguminosae during the vegetative stage." Soil Science and Plant Nutrition. 40. 485-495 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Osaki,M.: "^<14>C-Behavior of ^<14>C-[U]-sucrose,^<14>C-[U]-asparagin,and ^<14>C-[U]-serine introduced to the flag leaf of rice and sorghum plants during ripening." Soil Science and Plant Nutrition. 40. 637-646 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 但野 利秋: "低pH条件下における植物と土壌の相互作用(共著)" 博友社, 236 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tadano,T.: "Secretion of acid phosphatase by the roots of crop plants under phosphorus deficient conditions and some properties of the enzyme secreted by lupin roots." Plant Nutrition from Genetic Engineering to Field Practice. 99-102 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 但野利秋: "低PH条件下における植物と土壌の相互作用(共著)" 博友社(印刷中), (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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