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Bio-organic Chemistry on Egg Diapause of Silkworm

Research Project

Project/Area Number 05453164
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionMie University

Principal Investigator

IMAI Kunio  Mie Univ.Fac.Bioresources Associate Professor, 生物資源学部, 助教授 (80109313)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Okitsugu  Nagoya Univ.Fac.Agriculture Professor, 農学部, 教授 (50023411)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsDiapause hormone / Silkworm / Diapause hormone derivatives / Egg diapause / Lipophilic peptides / SGNP / Chemical synthesis / 蚕 / 食道下神経節 / 休眠誘導活性 / 親脂性ペプチド / 休眠誘導活性物質
Research Abstract

SEARCH FOR NATURAL SGNPS AND NEW DIAPAUSE INDUCING SUBSTANCES
Natural alpha-, beta- and gamma-SGNP were searched for in the extract of the suboesophageal ganglions of silkworm. alpha- and gamma-SGNP were isolated and identified, and beta-SGNP was speculated to exist.
To investigate the new diapause inducing substances, the heads of male silk moths were collected. They were extrated with water and several organic solvents. Most of the extracts showed the diapause inducing activity, and the result suggested that the organ contains many kinds of active substances. They were purified by repeated chromatography and one of the active substances (VAP peptide) was isolated. It had unique structure, physicochemical feature and biological activity.
PEPTIDE SYNTHESIS AND BIOLOGICAL ACTIVITY OF SYNTHETIC PEPTIDES
Diapause hormone (DH) , SGNPs and a fragment analog of VAP peptide were chemically synthesized and the synthetic peptides were used for preparation of antibodies and for the preparation of derivatives.
A procedure to derivatize the N terminus of DH was developed, as the biological activity was reduced when free DH molecule was simply treated with the derivatizing reagents. Acylated derivatives, fluorescent and pigmented derivative and photoaffinity labeled derivative were successfully prepared.
The C terminal pentapeptides and their analogs were synthesized and their biological activities were compared to each other. The result suggested 1) amide structure at C terminus is important, 2) the tripeptide amide is the smallest unit for biological function, 3)modification of the unit enhances the biological acitivity.
The results of the present research indicated the possiblility to clarify the mechanism for diapause induction and to design and to create the artificial diapause inducing substances.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Y.Sato,K.Imai,H.Saito,M.Ikeda,O.Yamashita,et al.: "Precursor Polyprotein for Multiple Neuropeptides Secreted from Suboesop hageal Ganglion of the Silkworm,Bombyx mori : Characterization of cDNA for Diapause Hormone Precursor and Identification of New Peptides." Proc.Natl.Acad.Sci.USA,. 90. 3251-3255 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Ideda,H.Saito,K.Imai,Y.Sato,O.Yamashita,et al.: "Induction of Embryonic Diapause and Stimulation of Ovary Trehalase Activity in the Silkworm,Bombyx mori,by Synthetic Diapause Hormone." J.Insect.Physiol.39. 889-895 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Saito,R.Takeda,Y.Hayashi,K.Imai,O.Yamashita,et al.: "The Core and Complimentary Sequence Responsible for Biological Activity of the Diapause Hormone of the Silkworm,Bombyx mori." Peptide. 15. 1173-1178 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Ikeda,Y.Sato,H.Saito,K.Imai,O.Yamashita et al.: "Molecular Characterization of Ovary Trehalase of the Silkworm,Bombyx mori and lts Transcriptional Activation by Diapause Hormone" Biochim.Biophys.Acta. 1281. 366-374 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Shiomi,M.Ikeda,Y,Sato,K.Imai,O.Yamashita,et al.: "Induction of Non-diapause Eggs by Anti-diapause Hormone Rabbit Serum Injected into the Diapause Type of the Silkworm,Bombyx mori." J.Insect Physiol.39. 693-699 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Imai,K.Sugiura,T.Komiya,O.Yamashita: "Isolation and Partial Structure of a Unique Lipophilic Peptide,VAP peptide,from Heads of Male Silkworm Moths" Biosci.Biotech.Biochem.60. 355-357 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Sato, M.Oguchi, N.Menjyo, K.Imai, H.Saito, M.Ikeda, M.Isobe and O.Yamashita: "Precursor Polyprotein for Multiple Neuropeptides Secreted from Suboesophageal Ganglion of the Silkworm, Bombyx mori : Characterization of cDNA for Diapause Hormone Precursor and Identification of New Peptides." Proc.Natl.Acad.Sci.USA. 90. 3251-3255 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Ikeda, Z.-H.Su, H.Saito, K.Imai, Y.Sato, M.Isobe and O.Yamashita: "Induction of Embryonic Diapause and Stimulation of Ovary Trehalase Activity in the Silkworm, Bombyx mori, by Synthetic Diapause Hormone." J.Insect.Physiol.39. 889-895 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Saito, Y.Takeuchi, R.Takeda, Y.Hayashi, K.Watanabe, M.Shin, K.Imai, M.Isobe, and O.Yamashita: "The Core and Complimentary Sequence Responsible for Biological Activity of the Diapause Hormone of the Silkworm, Bombyx mori." Peptide. 15. 1173-1178 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Z-H.Su, M.Ikeda, Y.Sato, H.Saito, K.Imai, M.Isobe and O.Yamashita: "Molecular Characterization of Ovary Trehalase of the Silkworm, Bombyx mori and Its Transcriptional Activation by Diapause Hormone" Biochim.Biophys.Acta. 1281. 366-374 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Shiomi, Y.Ishida, M.Ikeda, Y,Sato, H.Saito, K.Imai, M.Isobe and O.Yamashita: "Induction of Non-diapause Eggs by Anti-diapause Hormone Rabbit Serum Injected into the Diapause Type of the Silkworm, Bombyx mori." J.Insect Physiol.39. 693-699 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Imai, K.Sugiura, T.Komiya, and O.Yamashita: "Isolation and Partial Structure of a Unique Lipophilic Peptide, VAP peptide, from Heads of Male Silkworm Moths" Biosci.Biotech.Biochem.60. 355-357 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Imai,Sugiura,Komiya,Yamashita: "Isolation and Partial Structure of a Unique Lipophilic Peptide, VAP Peptide,from Heads of Male Silkworm Moths" Biosci.Biotech.Biochem.60. 355-357 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Saito ら: "The core and complimentarysequence responsible for biologicalactivity of the diapausehor-mone of the silkworm,Bombyx mori" Peptide. 15. 1173-1178 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Ikeda ら: "Induction of non-dispauseeggs by anti-diapause hormone rabittserum injected into the dispause type of the silkworm,Bombyx mori" J.Insect Physiol. 39. 889-895 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Z-H Su ら: "Molecular characterization of ovary trehalase of the silkworm,Bombyx mori and its transcriptional activation by dispause hormone" Biochim.Biophys.Acta. 1218. 366-374 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Suwan ら: "Silkworm,diapause hormone,structure-activity relationships indispensable role of C-terminal amide" Insect Biohem.Molec.Biol.24. 1001-1007 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Sato ら: "Precursor polyprotein for multiple neuropeptides secreted from the suboesophageal ganglion of the silkworm Bombyx mori:Characterization of the cDNA encoding the diapause hormone precursor and identification of additional peptides" Proc.Natl.Acad.Sci.USA. 90. 3251-3255 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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