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Characteristics of vasopressin receptors in the central nervous system and their regulation mechanism by neuro-humoral systems

Research Project

Project/Area Number05454154
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionFukuoka University

Principal Investigator

KAMIYA Hiro-o  Fukuoka Univ., Dept.of Pharmacol.Prof., 薬学部, 教授 (90028366)

Co-Investigator(Kenkyū-buntansha) HONDA Kenji  Fukuoka Univ., Dept.of Pharmacol.Assist.Prof., 薬学部, 助手 (60140761)
SAITO Ryo  Fukuoka Univ., Dept.of Pharmacol.Assist.Prof., 薬学部, 助手 (80122696)
TAKANO Yukio  Fukuoka Univ., Dept.of Pharmacol.Assist.Prof., 薬学部, 助手 (50113246)
Project Period (FY) 1993 – 1994
Project Status Completed(Fiscal Year 1994)
Budget Amount *help
¥6,300,000 (Direct Cost : ¥6,300,000)
Fiscal Year 1994 : ¥1,200,000 (Direct Cost : ¥1,200,000)
Fiscal Year 1993 : ¥5,100,000 (Direct Cost : ¥5,100,000)
KeywordsVasopressin / Vasopressin receptor / Adrenal steroids / Tachykinin peptides / Aldosterone / Antidiuretic action / pituitary / 受容体拮抗薬 / バソプレシン受容体(V1+V2)
Research Abstract

1. Vasopressin (AVP) receptor subtypes in rat brain :
Recently, the non-peptide compounds OPC-21268 and OPC-31260 were developed as selective antagonists of the AVP V_1 and V_2 receptors, respectively. Therefore, to determine the subtype (s) of the AVP receptor in the brain, we examined the inhibitions of specific binding of [^3H] -AVP by the V_1 antagonist OPC-21268 and V_2 antagonist OPC-31260. [^3H]-AVP binding to rat hippocampal membranes was strongly inhibited by the V_1 antagonist OPC-21268.
In this study, we tested for AVP V_1a-and V_2-receptor mRNAs in the hippocampus of adult rats by a reverse transcription-polymerase chain reaction (RT-PCR) method. PCR product of the V_2 receptor mRNA was detected in the hippocampus, but that of V_1a receptor mRNA was not. We do not know why V_1a receptor mRNA was not detected in the hippocampus in this study. Possible explanations are that the sequence of the AVP receptor mRNA in the brain may not completely consistent with that of V_1a receptor mRNA in peripheral tissues.
2. The effect of adrenal steroids on AVP receptor in rat hipocampus :
The AVP receptor in the hippocampus is regulated by mineral corticoid, such as aldosterone and corticosterone.
3. Central water regulation : tachykinins-AVP system :
The tachykinin NK-3 receptor agonist, senktide, has an antidiuretic effect by stimulating AVP secretion from the pituitary gland through the NK-3 receptor in the paraventricular nucleus of hypothalamus.

Report

(3results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report

Research Products

(40results)

All Other

All Publications

  • [Publications] Matsumoto,H.:"Optimum cross-linking spacer length of dimeric neurokinin B analogs for interaction with NK-1 tachykinin receptors." Bull.Chem.Soc.Japan,. 66. 1-9 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Honda,K.:"Pharmacological profiles of muscarinic receptors in the longitudinal smooth muscle of guinea pig ileum." Japan.J.Pharmacol.62. 43-47 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saigo,A.:"Central administration of senktide,a tachykinin NK-3 agonist,has an antidiuretic action by stimulating AVP release in water-loaded rats." Neurosci.Lett.159. 187-190 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimohigashi,Y.:"Receptor-mediated specific biological activity of a β-amyloid protein fragment for NK-1 substance P receptors." Biochem.Biophys.Res.Comm.193. 624-630 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saito,R.:"Vasopressin V1 receptor in rat hippocampus is regulated by adrenocortical systems." Brain Res.646. 170-174 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Honda,K.:"The mechanism of muscarinic agonist-stimulated inositol phosphate formation in permeabilized ileal smooth muscle." Jpn.J.Pharmacol.65. 275-280 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hirasawa,A.:"Evedence for the existence of vasopressin V2 receptor mRNA in rat hippocampus." Biochem.Biophys.Res.Comm.205. 1702-1706 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimohigashi,Y.:"Discriminative affinity labeling of tachykinin NK-1 and NK-3 receptors." Peptide Chemistry. 1993. 337-340 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimohigashi,Y.:"Specific interaction of β-amyloid fragments with NK-1 substance P receptors." Peptide Chemistry. 1993. 341-344 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Honda,K.:"Changes in 3H-quinuclidinyl benzilate binding and protein synthersis in the striatum following chronic administrations of muscarinic agonist." Jpn.J.Pharmacol.67. 83-86 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saigo,A,:"Role of NK-1 receptor in central cardiovascular regulation in rats:studies on a novel non-peptide antagonist,CP-96,345,of substance P NK-1 receptor." Reg.Peptides. 46. 293-296 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takano,Y.:"The mechanism of central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the hypothalamus in rats." Reg.Peptides. 46. 360-363 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 神谷大雄: "レセプター -基礎と臨床- (編集:井村裕夫,岡哲雄,芳賀達也,岸本英爾)" 朝倉書店, (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 高野行夫: "わかりやすい薬物受容体の基礎(高柳-成編) タキキニン受容体" 薬業時報社, (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saigo, A.: "Role of NK-1 receptor in central cardiovascular regulation in rats : studies on a novel non-peptide antagonist, CP-96,345, of substance P NK-1 receptor." Reg.Peptides. 46. 293-296 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takano, Y.: "The mechanism of central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the hypothalamus in rats." Reg.Peptides. 46. 360-363 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Matsumoto, H.: "Optimum cross-linking spacer length of dimeric neurokinin B analogs for interaction with NK-1 tachkinin receptors." Bull.Chem.Soc.Japan. 66. 1-9 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Honda, K.: "Pharmacological profiles of muscarinic receptors in the longitudinal smooth muscle of guinea pig ileum." Japan, J.Pharmacol.62. 43-47 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saigo, A.: "Central administration of senktide, a tachykinin NK-3 agonist, has an antidiuretic action by stimulating AVP release in water-loaded rats." Neurosci.Lett.159. 187-190 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimohigashi, Y.: "Receptor-mediated specific biological activity of a beta-amyloid protein fragment for NK-1 substance P receptors." Biochem.Biophys.Res.Comm.193. 624-630 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saito, R.: "Vasopressin V_1 receptor in rat hippocampus is regulated by adrenocortical systems." Brain Res.646. 170-174 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Honda, K.: "The mechanism of muscarinic agonist-stimulated inositol phosphate formation in permeabilized ileal smooth muscle." Jpn.J.Pharmacol.65. 275-280 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hirasawa, A.: "Evedence for the existence of vasopressin V_2 receptor mRNA in rat hippocampus." Biochem.Biophys.Res.Comm.205. 1702-1706 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimohigashi, Y.: "Discriminative affinity labeling of tachykinin NK-1 and NK-3 receptors." Peptide Chemistry. 1993. 337-340 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimohigashi, Y.: "Specific interaction of b-amyloid fragments with NK-1 substance P receptors." Peptide Chemistry. 1993. 341-344 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Honda, K.: "Changes in ^3H-quinuclidinyl benzilate binding and protein synthesis in the striatum following chronic administrations of muscarinic agonist." Jpn.J.Pharmacol.67. 83-86 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Honda,K.: "Changes in ^3H-quinuclidinyl benzilate binding and protein synthesis in the striatum following chronic administrations of muscarinic agonist." Japan.J.Pharmacol.67. 83-86 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hirasawa,A.: "Evidence for the existence of vasopressine V_2 receptor mRNA in rat hippocampus." Biochem.Biophys.Res.Comm.205. 1702-1706 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Saito,R.: "Vasopressin V_1 receptor in rat hippocampus is regulated by adrenocortical systems." Brain Res.646. 170-174 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Honda,K.: "The mechanism of muscarinic agonist-stimulated inositol phosphate formation in permeabilized ileal smooth muscle." Japan.J.Pharmacol.65. 275-280 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 高野行夫: "循環器調節に対するタキキニン・ペプチドの関与" 循環制御. 16. 283-286 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimohigashi,Y.: "Specific interaction of β-amyloid fragments with NK-1 substance Preceptors." Peptide Chemistry. 1993. 341-344 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimohigashi,Y.: "Discriminative affinity labeling of tachykinin NK-1 and NK-3 receptors." Peptide Chemistry. 1993. 337-340 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsumoto,H.: "Optimum cross-linking spacer length of dimeric neurokinin B analogs for interaction with NK-1 tachykinin receptors." Bull.Chem.Soc.Japan. 66. 1-9 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Honda,K.: "Phamacological profiles of muscarninic receptors in the longitudinal smooth muscle of guinea pig ileum." Japan.J.Pharmacol.62. 43-47 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Saigo,A.: "Central administration of senktide,a tachykinin NK-3 agonist,has an antidiuretic action by stimulating AVP release in water-loaded rats." Neurosci.Lett.159. 187-190 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimohigashi,Y.: "Receptor-mediated specific biological activity of a beta-amyloid protein fragment for NK-1 substance P receptors." Biochem.Biophys.Res.Comm.193. 624-630 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Saigo,A.: "Role of NK-1 receptor in central cardiovascular regulation in rats:studies on a novel non-peptide antagonist,CP-96,345,of substance P NK-1 receptor." Reg.Peptides. 46. 293-296 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takano,Y.: "The mechanism of central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the hypothalamus in rats." Reg.Peptides. 46. 360-363 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高野,行夫: "非ペプチド性サブスタンスP拮抗薬" Clinical Neuroscience. 11. 109 (1993)

    • Related Report
      1993 Annual Research Report

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Published : 1993-04-01   Modified : 2016-04-21  

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