|Budget Amount *help
¥4,700,000 (Direct Cost : ¥4,700,000)
Fiscal Year 1994 : ¥1,500,000 (Direct Cost : ¥1,500,000)
Fiscal Year 1993 : ¥3,200,000 (Direct Cost : ¥3,200,000)
We employed EBV-induced infectious mononucleosis (IM) as a model of activated T cell death to elucidate cellular requirements for induction of T cell death by activation with viral infection. Obtained results are as follows :
1)Both activated T cells in acute IM patients and memory T cells in normal persons express CD45RO and Fas antigen, which can mediate apoptosis. Unlike memory T cells, activated T cells in IM easily undergo apoptotic cell death after on a simple incubation in vitro. By immunizing mice with IM cells, we obtained a novel mouse monoclonal antibody, termed IMN3.1, which was marked to react with apoptosis-prone T cells. Molecular cloning of IMN3.1-identified antigen is in progress.
2)Seemingly supporting their susceptibility to apoptosis, activated T cells in IM lacked expression of Bcl-2, which have a preventive function against apoptotic cell death. Low or absent expression of Bcl-2 was observed on granulocytes and monocytes, both of which have shorter life-spans. The important finding was that anti-Fas antibody could accelerated apoptotic cell death in granulocytes and monocytes. These observations suggest that the Fas antigen/ligand system may play a key role in resolution of inflammatory and immune responses.
3)The mutation of the p53 oncogene is thought to lead to oncogenosis in human malignancies. Expression of p53 was not found in activated T cells in IM patients, although they were sucseptible to apoptosis. Ionizing irradiation could induce p53 expression on the whole population of peripheral blood lymphocytes, concomitant with marked apoptosis. However, we found a marked difference of lymphocyte subpopulations regarding p53 induction. Induction of p53 in CD4^+ T,CD8^+ T and B cells after irradiation was prominent. In contrast, neither TCR-gamma/delta^+ T cells nor NK cells showed identifiable levels of p53. The results suggest that radiation-induced lymphocytic apoptosis may be mediated by p53-dependent or-independent mechanisms.