|Budget Amount *help
¥6,600,000 (Direct Cost : ¥6,600,000)
Fiscal Year 1994 : ¥2,600,000 (Direct Cost : ¥2,600,000)
Fiscal Year 1993 : ¥4,000,000 (Direct Cost : ¥4,000,000)
In this study, we raised 4 kinds of monoclonal antibodies against human renin, which recognized 4 different epitopes, respectively. However, all 4 andtibodies recognized both the active and inactive forms of renin, i.e., total renin, and none of them were specific to the active form only, or to the inactive form only. Hence, we established an immuno-radiometric assay (IRMA) system, which can determine the plasma levels of total renin (active+inactive form) using two monoclonal antibodies (clone numbers 12-12 and 69-30), Of the 4 antibodies raised, the only one (69-30) was shown to react with the renin within the formalin-fixed, paraffin-enbedded pathology specimens.
Using this particular antibody, we performed immunohistochemical studies on various renal lesions, such as Wilms tumors, congenital mesoblastic nephromas (CMN), clear cell sarcomas of kidney (CCSK), and multicystic dysplastic kidneys (MCDK), which revealed the primiary reninism in Wilms tumor and MCDK,and secondary reninism in CMN and CCSK,respectively.
Plasma renin levels (PRL) were determined by the IRMA system in normal children, and it was shown that PRL was high in early infancy, and was gradually decreasing with age. It alsw demonstrated that PRLs in Wilms tumor patients well reflected the extent and the volume of the tumor, indicating that PRL can be utilized as a tumor marker.