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Neuron circuit analysis on the basis of neuroactive substances and receptors

Research Project

Project/Area Number 05454658
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIZUNO Noboru  Kyoto Univ., Fac.of Med., Prof., 医学研究所, 教授 (10025596)

Co-Investigator(Kenkyū-buntansha) NOMURA Sakashi  Kyoto Unoiv., Coll.Med.Technol., Prof., 医療技術短期大学部, 教授 (60034188)
TOKUNO Hironobu  Kyoto Univ., School of Med., Res.Assoc., 医学研究科, 助手 (40212071)
TAKADA Masahiko  Kyoto Univ., School of Med., Lecturer, 医学研究科, 講師 (00236233)
KANEKO Takeshi  Kyoto Univ., School of Med., Assoc.Prof., 医学研究科, 助教授 (90177519)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywordscerebral cortex / striatum / cerebellum / pain / endogenous paincontrol system / opioid receptor / metabotropic glutamate receptor / substance P receptor / グルタミン酸合成酵素 / K物質受容体 / 排尿中枢 / 顎運動 / 迷走神経核群 / NMDA受容体 / P物質 / セロトニン / ドパミン / GABA / 網膜
Research Abstract

1.An antibody against substance P receptor (SPR) was raised, and distribution of SPR in the central nervous system (CNS) of the rat was examined immunohistochemically. The vast majority of cerebral cortical neurons with SPR were GABAergic non-pyramidal neurons. Striatal neurons with SPR were segregated into somatostatinergic and cholinergic apiny (intrinsic) neurons.
2.Distributions of mRNAs for metabotropic glutamate receptors, mGluR2-mGluR7 in the rat CNS were examined by in situ hybridization. Antibodies against mGluR2, and mGluR4 - mGluR8 were raised. Presynaotic localization of m GluRs were immunohistochemically observed light and electron microscopically in the cerebellar cortex (mGluR2 and mGluR4) , layr I of the spinal cord (mGluR7) and olfactory tubercle (mGluR8).
3.Two kinds of antibodies against mu-opioid receptor (MOR) were raised, and distribution of MOR in the rat CNS was examined immunohistochemically. Presynaptic localization of MOR was observed light and electron microscopically in the medial terminal nucleus of the accessory optic tract, nucleus of the solitary tract, and layr I of the spinal cord. In layr I of the spinal cord, some axon terminalswith substance P contained MOR.
4.Analysis was performed on the local circuitry in the cerebral cortex, striatum and cerebellar cortex, the nociceptive system, the endogeous pain-control system, and masticatory system.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] 金子 武嗣: "Morphological and chemical characteristis of substance P receptor-immunoreactive neurons in the rat neocortey" Neuroscience. 60. 191-211 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大石 仁: "Immunohistochemical localization of metabotropic glutamate receptors, mGluR2 and mGluR3, in rat cerebellar cortex" Neuron. 13. 55-66 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 金子 武嗣: "Glutamate-synthesizing enzymas in GABAergic neurons of the neocortex: a double immunofluorescence study in the rat" Neuroscience. 61. 839-849 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大石 仁: "Distributions of the mRNAs for L-2-amino-4-phasphonobutyrate-sensitive metabotropic glutamate receptors, mGluR4 and mGluR7, in the rat brain" J. Comp. Neurol. 360. 555-570 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 金子 武嗣: "Glutaminase-positive and glutaminase-negative pyramidelcells in layenVI of the primary motor and somotosensory cortices: a combined analysis by intracellular staining and immunocy to chemistry in the rat" J. Neurosci. 15. 8362-8377 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野村 嶬: "Localization of μ-opioid receptor-like immunoreactivity in the central components of the vegus nerve: a light and electron microscope study in the rat" Neuroscience. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneko, Takeshi: "Morphological and chemical characteristics of substance P receptor-immunoreactive neurons in the rat neocortex" Neuroscience. 60. 191-211 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohishi, Hitoshi: "Immunohistochemical localization of metabotropic glutamate receptors, mGluR2 and mGluR3, in rat cerebellar cortex" Neuron. 13. 55-66 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneko, Takeshi: "Glutamate-synthesizing enzymes in GABAergic neurons of the neocortex : a double immunofluorescence study in the rat" Neuroscience. 61. 839-849 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohishi, Hitoshi: "Distributions of the mRNAs for L-2-amino-4-phosphonobutyrate-sensotive metabotropic glutamate receptors, mGluR4 and mGluR7, in the rat brain" J.Comp.Neurol.360. 555-570 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneko, Takeshi: "Glutaminase-positive and glutaminase-negative pyamidal cells in layr VI of the primary motor and somatosensory cortices : a combined analysis by intracellular staining and immunocyto-chemistry in the rat" J.Neurosci.15. 8362-8377 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nomura, Sakashi: "Localization of mu-opioid receptor-like immunoreactivity in the central components of the vagus nerve : a light and lectron microscope study in the rat" Neuroscience. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 李雲慶: "Premptor neurons for trigeminal motor nucleus neurons innervating the jaw-closing and jaw-opening muscles: Differential distribution in the lowor brainstem of the rat" J. Comp. Neurol.356. 563-579 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 丁玉強: "Direct prajectiors from the dorsolateral pantine togmentum to pudendal motoneurons innervating the external urethral sphinctor muscle in the rat." J. Comp. Neurol.357. 318-380 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大石仁: "Distribution of the mRNAs for L-2-amino-4-phosphonobutyrate-sensitive metabotropic glutamate receptors, mGluR4 and mGluR7, in the rat brain" J. Comp. Neurol.360. 555-570 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 金子武嗣: "Glutaminase-positive and glutaminase-negative pyramidal cells in layer VI of the primary metor and somatosensory cartice cortices: A combined by intracellular staining and immnocytochemistry in the rat" J.Neurosci.15. 8362-8377 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 丁玉強: "Localization of the neuromedin K. receptor (NK3) in the central nervous system of the rat" J. Comp. Neurol.364. 290-310 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野村嶬: "Localization of U-opioid receptor-like immunoreactivity in the central components of the vagus nerve: A light and electron microscope stady in the rat" Neuroscience. 印刷中. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大石 仁: "Immunohistochemical localization of metabotropic glutamate receptors,mGluR2 and mGluR3,in rat cerebellar Cortex" Neuron. 13. 55-66 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 金子 武嗣: "Glutamtae-synthesizing enzymes in GABAergic neurons of the neocortex:a double immunvfluorescence study in the rat" Neuroscience. 61. 839-849 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中谷 嘉文: "Immunolistochemical localization of substance P receptor in the ceniral nervous system of the adult rat" J.Cvmp.Neurol.347. 249-274 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 赤沢 千尋: "Differential expression of five N-methyl-D-aspartate rercptor subunt mRNAs in the cerebellum of develvping and adult rats" J.Comp.Neurol.347. 150-160 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 金子 武嗣: "Immunocytochemical localization of μ-opioid receptor in the rat caudatte-putamen" Neurosci.Lett.184. 149-152 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大石 仁: "Distribution of the mRNAs for L-2-anino-4-Phosphonobutyrate-sensitive metabotropic glutamate receptors,mGluR4 and mGluR7,in the out brain" J.Comp.Neurol.(印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大石 仁: "Distribution of the messenger RNA for a metal-otropic glutamate receptor,mGluR2,in the central nervous system of the rat" Neuroscience. 53. 1009-1018 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 李 雲慶: "The sites of origin of dopaminergic afferent fibers to the lateral halenular nucleus in the rat" J.Comp.Neurol.333. 118-133 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 大石 仁: "Distribution of the mRNA for a metal-otropic glutamate receptor(mGluR3)in the rat brain.An in situ hybridization study" J.Comp.Neurol.335. 252-266 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 重本 隆一: "Immunohistochemical localization of a metal-otropic glutamate receptor,mGluR5,in the rat brain" Neurosci.Lett.163. 53-57 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 金子 武嗣: "Morphological and Chemical Characteristics of substance P receptor-immunoreactive neurons in the rat neocortex" Neuroscience. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 赤沢 千尋: "Expression of mRNAs of L-AP4 sensitive metal-otropic glutamute receptors(mGluR4,mGluR6,mGluR7)in the rat retina" Neurosci Lett.(印刷中). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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