| Project/Area Number |
05557021
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IWAMOTO Aikichi University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10133076)
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| Co-Investigator(Kenkyū-buntansha) |
SHUIMIZU Yohko University of Tokyo, Faculty of Medicine, As sociate Professor, 医学部(医), 助教授 (00142357)
YOSHIKURA Hiroshi University of Tokyo, Faculty of Medicine, Professor, 医学部(医), 教授 (60012754)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥9,500,000 (Direct Cost: ¥9,500,000)
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| Keywords | hepatitis C virus / HCV / neutralizing antibodies |
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| Research Abstract |
We found that HPB-Ma cells are suitable for culturing HCV in vitro. Efficiency of HCV replication are different among subclones of HPB-Ma cells and we established HPB-Ma clone 10 which supports HCV replication very efficiently. We measured the infectivity in vitro of the samples whose Chimpanzee infective titer had been titrated previously and found the correlation between the infectivity of hepatitis C virus in vitro and in vivo. When we innoculated a patient's plasma which contained 6HCV quasispecies, the same two were recovered from the plasma of the infected chimpanzee and culture in vitro. The result confirmed further the correlation between the infectivity in vitro and in vivo.
We established the method to measure the efficiency of adsorption of HCV to the cells and found the correlation between the adsorption eficiency and the infectivity. HCV in the sample with a low adsorption eficiency was present as immune complex with antibodies.
We made a chimeric env gene between HCV and Moloney murine leukemia virus, however, the product stayd in the rough endplasmic reticulum and was not expressed on the cell surface.
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