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Novel Carrot Embryo-forming System.

Research Project

Project/Area Number 05640723
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

MASUDA Hiroshi  Obihiro Univ.of Agric.and Vet.Med., Dep.Bioresource Chem., Associate Professor, 畜産学部, 助教授 (00003111)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsCarrot / Concanavalin A / 2,4-Dichlorophenoxyacetic acid / Somatic Embryogenesis / Methyl-Jasmonate / コンカナバリンA / 2,4-ジクロロフェノキシ酢酸 / ニンジン胚軸 / コルチチン
Research Abstract

We have developed a novel embryo-forming system in which carrot somatic embryogenesis takes place in a one-stage process from epidermal cells in the absence of 2,4-D,provided hypocotyl explants are incubated in a liquid MS medium containing 2,4-D for 12-24 hours. In addition, in this system, somatic embryos develop directly via formation of cluster cells by repeating division of organized epidermal cells with adhesion to explants, without dedifferentiation to callus and suspension cultured cells. Somatic embryogenesis occurred directly from carrot epidermal cells was observed using scanning electron microscopy.
Epidermal cells peeled off from parent tissues were present as compressed cells and then expanded to become tubelar cells. Subsequently, tubular cells were regularly and horizontally divided to from linear cluster cells and then these cluster cells divided vertically to become enlargment. Swelling structure were formed by irregular and oblique division at places on linear cluster cells and then continued to divide to form small proembryos. Some cells which comprised surface of swelling structure and proembryos expanded to every direction to become covered on surface of structure. These expanding cells made probably put a layr of surface of structure and furthermore piled up cell layr to form from swelling to globular-stage embryo via small proembryo. These expanding cells were formed on surface of heart-stage embryo.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Hiroshi Masuda,Shin-Ichi Oohashi,Yoshihiko Tokuji and Yuke Mizue: "Direct embryofurmadion from epiderural cells of carrot bypocstyls." Jounal plant pluysiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Masuda, S.Oohashi, Y.Tokuji and Y.Mizue: "Direct embryo formation from epidermal cells of carrot hypocotyls" Journal Plant Physiology. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroshi Masuda,Shin-Ichi Oohashi,Yoshihiko Tokuji and Yuka Mizue: "Direct ernbryo formation from epidermal cells of Carrot bypocstyls." Jonrnal Plant Plysiology. (in press).

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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