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Exploitation of new methods for efficent production of transgenic animals

Research Project

Project/Area Number05660323
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionThe University of Tokyo

Principal Investigator

TOJO Hideaki  The University of Tokyo, Faculty of Agricclture, Associate Professor, 農学部, 助教授 (20041668)

Project Period (FY) 1993 – 1994
Project Status Completed(Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1994 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1993 : ¥1,400,000 (Direct Cost : ¥1,400,000)
KeywordsTransgenic / Bovine embryos / PCR / Farm animals / 制限酵素
Research Abstract

Transgenic technology has provided powerful tools for the examination of the regulation of gene expression in cellular and physiological funtions. Transgenic farm animals havd been generated for improved products or productivity of domestic animals and resistance to animal diseases. (1) The most common method used for gene teansfere in farm animals is microinjection of DNA into pronuclei of zygotes. However, overall efficiency of transgenic farm animals were extreamely lower than that of miceowing to low integration of foreing DNA into host DNA.The present study was designed to improve the efficiency of generation of transgenic farm animals. (2) To improve the integration rate of for eign DNA into host DNA,the restriction enzyme with DNA was microinjected into the pronuclei of zygotes to induce the breaks in host genome DNA which increase the opportunity of integration of foreign DNA inothost DNA.In 8 concentrations from 10^<-4> to 10IU of EcoRI,The 10^<-1.5> and 10^<-2> IU were most effective to appropriate break in host genome DNA.To establish the method for selecting the transgenic embryos before they are transffered to the recipient, DpnI and Bal 31 enzymes were used for digestion of DNA fragement that not integrated into the host genome in the embryos. After this treatment, integrated gene was amplified by PCR.The results showed that this method was useful for selection of transgenic embryos. (3) As a basic experiment for methodological improvement of production rate of transgenic farm animals, the in vitro maturation, fertilization and culture of bovien oocytes cololected from the ovarian follicles of carcus were sutdied. It was found that individula differences in frozen semen remarkably influenced on the successful efficiency of in vitro fertilization.

Report

(3results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report

Research Products

(31results)

All Other

All Publications

  • [Publications] H. Tojo, T. Tanaka, S. Matsuzawa, etal: "Production and characterization of transgenic mice expressing a hGH gene..." Journal of Reproduction and Development. 39. 145-155 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K. Katsube, H. Tojo, Y. Sakaguchi, et al: "Human fetal-adult globin gene switching in transgenic mice;persisitent......" Biochem. Biophysi. Res. Commun.194. 146-152 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H. Nagawawa, M. Hasegawa, et al: "Normal and neoplastic growth of mammary glands and circulating levels of......" Zoological Science. 10. 967-970 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S. Tanaka, H. Tojo, C. Tachi: "Possible involvement of amiloride-sensitive Na+/H+ exchanger and/or....21GC04:Journal of Reproduction and Development" 39. 97-107 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A. Ikeda. S. Matsuyama, Nishihara: "Changes in endogenous growth hormone secretion and onset of puberty in...." Endocrine Journal. 41. 525-529 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S. Tanak, K. Kasai, H. Tojo, T. Sawasak: "Melanocytes fail to survive in hair bulbs of the shiba goat (Capra hicus)..." Pigment Cell Research. 7. 152-257 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 家畜繁殖学会編: "新繁殖学辞典" 文永堂出版,東京, 609 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 大沢利昭・小山次郎他編: "免疫学辞典" 東京化学同人, 616 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tojo, et al.: "Production and characterization of transgenic mice expressing a hGH fusion gene driven by the promoter of mouse whey acidic protein (mWAP) putatively specific to mammary gland" J.Reprod.Develop.39. 145-155 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Katsube, et al.: "Human fetal-adult globin gene switching in transgenic mice : persistent expression of the Gr-globin gene in the Japanese HPFH." Biochem.Biophysi.Res.Commun.194. 246-152 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Nagasawa, et al.: "Normal and neoplastic grwoth of mammary glands and circulating levels of prolactin and growth hormone in mouse whey acidic protein promotor/human growth hormone (mWAP/hGH) transgenic mice." Zool.Sci.10. 963-970 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Tanaka, et al.: "Possible involvement of amiloride sensitive Na^+/H^+ exchanger and/or Na^+ transport systems in hatching trophoblast spreading in mouse blastocysts cultured in vitro." J.Reprod.Develop.39. 97-107 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Ikeda, et al.: "Changes in endogenous growth hormone secretion and onset of puberty in transgenic rats expressing human growth hormone gene." Endocrine J.41 (5). 525-529 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Tanaka, et al.: "Melanocytes fail to survive in hair bulbs of the Shiba goat (Capra hircus) with the dominat black-eyed white phenotype." Pigment Cell Res.7. 152-157 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Society of Animal Reproduciton, ed., New Dictionary of Reproduction. Buneidou Publishing Inc, Tokyou, 609 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Oosawa ed., Dictionary of Immunology. Kagaku-dojinn Publishing Inc, 616 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tojo,S.Tanaka,S.Matsuzawa,et al: "Production and characterization of transgenic mice expressing a hGH fusion gene driven by the promoter.." Journal Reproduction and Development. 39. 145-155 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Katsube,H.Tojo,Y.Sakaguchi,et al: "Human fetal-adult globin gene switching in transgenic mice;persistent expression of the Gr-globin gene...." Biochem.Biophysi.Res.Commun.194. 146-152 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Nagazawa,M.Hasegawa,K.Yamamoto et al: "Normal and neoplastic growth of mammary glands and circulating levels of prolactin and growth hormone.." Zoological science. 10. 967-970 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Tanaka,H.Tojo,C.Tachi: "Possible involvement of amiloride-sensitive Na+/H+exchanger and/or Na+ transpoter systems in hatching.." Journal Reproduction and Development. 39. 97-107 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Ikeda,S.Matsuyama,M.Nishihara,H.Tojo: "Changes in endogenous growth hormone secretion and onset of puberty in transgenic rats expressing human.." Endocrine Journal. 41. 525-529 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Tanaka,K.Kasai,H.Tojo,T.Sawasaki,: "Melanocytes fail to survive in hair bulbs of the Shiba goat(Capra hircus)with the dominant black-eyed.." Pigment Cell Research. 7. 152-257 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 家畜繁殖学会編: "新繁殖学辞典" 文永堂出版,東京, 609 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大沢利昭・小山次郎他編: "免疫学辞典" 東京化学同人, 616 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Nagasawa,K.Yamamoto,N.Nagumo,H.Tojo: "Normal and neoplastic growth of mammary glands and circulating levels of prolactin and growth hormone" Zoological Sciences. 10. 963-970 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Tojo,S.Tanaka,A.Matsuzawa,C.Tachi: "Production and characterization of transgenic mice expressing a hGH fusion gene driven by the promoter" Journal of Reproduction and Development. 39. 145-155 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Sakaguchi,T.Tojo,Y.Fukumaki: "Human fetal-adult globin gene switching in tramsgenic mice;persistent expression of the Gr-globin gene" Biochem.Biophysi.Res.Commun.194. 246-252 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Tanaka,H.Tojo,S.Taci: "Possible involuvement of amirolide-sensitive Na/H exchanger and/or Na^+ transport systems in hatching" Journal of Reproduction and Development. 39. 97-107 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 田中慎・東條英昭・松沢照雄: "ヒト成長ホルモン(hGH)遺伝子導入マウスの下垂体" 実験動物. 42. 475-479 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 東條英昭: "トランスジェニック動物の生産-家畜への遺伝子導入-" 日本畜産学会報. 64. 34-40 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 奥田研爾・大沢利昭他: "免疫学辞典" 東京化学同人, 616 (1993)

    • Related Report
      1993 Annual Research Report

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Published : 1993-04-01   Modified : 2016-04-21  

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