|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1995 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1994 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1993 : ¥1,000,000 (Direct Cost : ¥1,000,000)
Na, K-ATPase, also called the sarcolemmal Na^+-pump, is a ubiquitous transmembrane protein of mammalian cells that maintains electrochemical Na^+ and K^+ gradients across the plasma membrane and affects excitability, contractility, and cell volume regulation of various kinds of cells. The Na, K-ATPase protein comprises two subunits : a large catalytic alpha-subunit and a smaller glycosylated beta-subunit of unknown function. At least three alpha-subunit isoforms, alpha1, alpha2, and alpha3, have been characterized in rats and humans. Two beta-subunit isoforms, beta1 and beta2, have been identified in rats.
In the present research project, we investigated the effects of sodium ion and various hormones on Na, K-ATPase alpha1 and beta1 mRNA expression in the cardiovascular system. Na, K-ATPase mRNA expression was analyzed by Northern blotting and Na^+-pump protein content was analyzed by enzyme-linked immunosorbent assay (ELISA). We have revealed that sodium ion, thyroid hormone, and angiotensin II stimulate Na, K-ATPase alpha1-and beta1-isoform mRNA and protein expression in rat vascular smooth muscle cells, mesangial cells, and cardiac myocytes and regulate contractility, excitability, and volume regulation of these cells.
(i)ラット培養血管平滑筋細胞にangiotensin IIを添加すると、NaポンプmRNAおよびタンパクの発現が増加した.また、Naポンプ遺伝子の5^′上流領域に、angiotensin IIの作用部位が存在する可能性が示唆された(Am J Physiol 267:H1295-H1302,1994).