|Budget Amount *help
¥1,800,000 (Direct Cost : ¥1,800,000)
Fiscal Year 1994 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1993 : ¥1,300,000 (Direct Cost : ¥1,300,000)
The objective of this study is to evaluate the effective and safe preservation period of the irradiated blood products and to establish a blood usage guideline after irradiation in order to effectively utilize the irradiated blood.
Fresh whole blood and concentrated red cells stored in MAP solution (RC-MAP) were used for the study. Fresh whole blood was irradiated with dose of 5 and 15 Gy. Lymphocyte activity (PHA and Con-A stimulation) and influences on red cells were assayd. RC-MAP was irradiated with dose of 15 and 30 Gy at 1,7 and 14 days after blood collection. Electrolytes (Na, K,CL) and plasma hemoglobin concentration were assayd in RC-MAP irradiation.
Fresh whole blood with 15 Gy irradiation can be used within 21 days with a caution of the level of potassium. Irradiation of 5 Gy dose may be enough to inactivate lymphocyte function for a whole blood preserved 7 days. After irradiation to RC-MAP,plasma potassium levels were increased. Effective preservation period of RC-MAP should be shortened for RC-MAP with a longer preservation period before irradiation and a higher dose irradiation. Irradiation of 15 Gy for 1 day, 7 days, and 14 days preservation RC-MAP should be used within 14 days, 7 days, and 3 days after irradiation, respectively, due to the elevated levels of potassium.
Effective preservation period of blood products after irradiation for the prevention of GVHD should be decided depending upon the type of blood product, the dose of radiation and the preservation period before irradiation. After the establishment of a blood usage guideline after irradiation, effective utilization of irradiated blood products can be achieved.