HIDA Jin-ichi Kinki University, assistant, 医学部, 助手 (20238306)
YASUTOMI Masayuki Kinki University, professor, 医学部, 教授 (60028438)
TANAKA Akira Kinki University, assistant professor, 医学部, 講師 (60179736)
|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1995 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1994 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1993 : ¥700,000 (Direct Cost : ¥700,000)
In immunohisochemical study, we have revealed that 35% of colorectal cancer is laminin staining positivity in basement membrane (BM). And we have found that basement membrane producing cancer (BMPC) have been higher frequency of liver metastasis than colorectal cancer without BM (non-BMPC). As for recurrence rate of liver metastasis, the former is 67%, the latter is only 8%. We have examined risk of liver metastasis using BM analysis for laminin, and indicated correlation between BMPC and liver metastasis of colorectal cancer (sensitivity : 75.6%, specificity : 85.7%, accuracy : 83.9%, negative-value : 94.2%) according to that, we could predict risk of liver metastasis.
Furthermore, it was seen two types BMPC in immunohistochemical study. One is BM present around each cancer nest (type A), and other was BM around several cancer nests (type B). A significant differences were found between type A and type B in rate of liver metastasis (85.7% and 41.2%, respectively). As compared primary c
ulture cells of colorectal cancer, we found that the BM was produced by cancer cells themselves. Next, we have examined correlation between ability of producing BM and their laminin receptor (VLA-6). The expression of VLA-6 was seen in cytoplasms of cancer cells both BMPC and non-BMPC.Therefore it might be seen that ability of producing BM was independent of laminin receptor.
We performed hepatic arterial infusion therapy using mitomycin-C (MMC) and 5-fluorouracil (5-FU) in postoperative patients to protect liver metastasis. The recurrence rate of liver metastasis in hepatic arterial infusion group and in control group were 5.3% and 14.2%, respectively. In BMPC, recurrence rate of liver metastasis in hepatic arterial infusion group and in control group was 16.7% and 66.7%, respectively.
In the future, for elucidated as the machanisms of the liver metastasis of colorectal cancer, we make model rat of natural liver metastasis. And we will perform immunochemical intraarterial therapy with interleukin 2 for prevention of liver metastasis. Less