| Project/Area Number |
05671182
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Keio University |
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Principal Investigator |
KAWASE Takeshi Department of Neurosurgery, School of Medicine, Keio University ; Assistant Professor, 医学部, 講師 (40095592)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Noriyuki Department of Neurosurgery, School of Medicine.Keio University ; Assistant, 医学部, 助手 (80220268)
SHIMIZU Katsuyoshi Department of Neurosurgery, School of Medicine.Keio University ; Assistant, 医学部, 助手 (80216085)
MITANI Shinji Department of Neurosurgery, School of Medicine.Keio University ; Assistant, 医学部, 助手 (90200047)
HORIGUCHI Takashi Department of Neurosurgery, School of Medicine, Keio University ; Assistant, 医学部, 助手 (70245520)
OGINO Masahiro Department of Neurosurgery, School of Medicine, Keio University ; Assistant, 医学部, 助手 (80224137)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Hypothermia / Cerebral Ischemia / Immediate Early Genes / Delayd Meurnal Death / Cerebral Blood Flow / Adenosine / Cisternal Irrigation / Cerebral Metabolism / Cerebral Irrlgation / brain hypotermia / perfusion model / cat |
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| Research Abstract |
Methods Transient forebrain ischemia was produced in Mongolian gerbils (1), and Wistar rats(2), which were respectively divided into normthermic and hypothermic groups. Delayd neuronal death was evaluated in the hippocampal region. Stress protein and c-Fos/c-Jun products were analyzed by immunohistochemistry and Western blotting. Carbon-fourteen leucine autoradiography was also performed (1). Extracellular concentrations of dopamine (DA), adenosine (ADO) and their metabolites in the striatum were measuredusing in vivo microdialysis (2). Cisternal irrigation of cold artificial cerebrospinal fluid (ACSF) were performed in the cat. Cerebral temperature, cerebral blood flow (CBF), blood gas analysis (BGA) and systemic blood pressure (SBP) were measured (3).
Results (1) Hypothermia reducedstress response after ischekmia and produced early recovery of protein synthesis, which can enhance immediate early gene expression. Increasing c-Jun synthesis could be one of the protective mechanisms and/or survival markers effected by mild hypothermia against delayd neuronal death. (2) Intraischemic hypothermia reduced the release of DA and inhibited the uptake of ADO.Postischemic hypothermia reducedthe metabolism and elevated the concentration of ADO.(3) Cold ACSF irrigation provides 2-3ﾟC of focal hypothermia but had no effect on SBP or BGA.Transient decrease of CBF was observedin few cases.
Conclusion Focal hypothermia provided by cisternak irrigation of cold ACSF could be a beneficial treatment for cerebralischemia, especially in combination with supportive modalities mentioned above.
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